Siriwodee CHOMDEJ, Woroluk SAOKEAW, Ki'r'risok BUDDHACHAT, Waranee PRADİT, Puntita SIENGDEE, Sittidet MAHASAWANGKUL, Supophen SRIPIBOON, Cholermchor'r SOMGIRD, Koroko'r NGANVONGPANIT, Siriwon ONGCHAI, Chotcho're THITARAM

Asya Filinde (Elephas maximus) Biglikanın Tanımlanması, Karakterizasyonu ve Ekspresyon Analizi

Bu çalışmanın amacı Asya filinde biglikan kodlayan sekans ve sonuçlanan amino asit sekansı ile farklı dokulardaki ekspresyonunu araştırmak ve yara iyileşmesindeki durumunu incelemektir. Elde edilen sonuçlar Asya fili biglikan kodlayan sekansın 1110 baz çifti (Ulaşım Numarası: JQ753329) olduğunu ve 369 amino asit kodladığını göstermiştir. Asya ve Afrika filleri arasında kodlama ve amino asit sekansları birbirleriyle sırasıyla %99 ve %98 oranında benzerlik gösterdi. Biglikan proteinde korunmuş domainin varlığı gözlemlendi. Ayrıca biglikan ekspresyonu 15 farklı dokuda tespit edilirken kıkırdak ve dalakta baskın olarak eksprese edildiği belirlendi. Yara iyileşmesindeki ekspresyon analizinde biglikan mRNA seviyesinin yaş, yara tipi ve devresi gibi birçok faktör tarafından etkilendiği tespit edildi.

Identification, Characterization and Expression Analysis of Biglycan in Asian Elephant (Elephas maximus)

The aims of this study were to investigate the coding sequence and the deduced amino acid sequence of Asian elephant's biglycan gene as well as its expression in different tissues and conditions using wound healing as a model. The results showed that Asian elephant biglycan coding sequence was 1,110 base pair (bp) long (accession number: JQ753329), encoding 369 amino acids. The coding and amino acid sequences between Asian and African elephants revealed 99% and 98% similarity, respectively. The conserved domains of biglycan protein were also observed. In addition, its expression was found in 15 tissues with a predominant expression in cartilage and spleen. For expression analysis in the wound healing process, it was found that the level of biglycan mRNA was influenced by many factors, including age, type of wound and stage of wound healing.

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