Effect of Quercetin on Cortisol and Oxytocin Levels, Oxytocin Receptor Gene Expression and Morphometry of Uterus in Rats Exposed to Bisphenol A

Bu çalışma birlikte ve ayrı ayrı kuersetin ve bisfenol A (BPA) uygulamalarının ratlarda plazma kortizol ve oksitosin seviyelerine, oksitosin reseptör gen ekspresyonuna ve uterus morfometrisine etkilerini araştırmak amacıyla yapılmıştır. Bir haftalık alıştırma dönemi sonrasında 20 dişi rat dört gruba ayrıldı. Kontrol grubuna intraperitoneal etanol-salin enjeksiyonu ile birlikte hidroksipropilmetilselüloz (HPMC) gavaj yoluyla uygulandı. İkinci gruba 4 hafta boyunca haftada iki kere olmak üzere etanol-salinde çözdürülmüş BPA 50 µg/vücut ağırlığı dozunda enjekte edildi. Üçüncü gruba 4 hafta boyunca haftada iki kere olmak üzere HPMC içinde çözdürülmüş edilmiş kuersetin 30 mg/vücut ağırlığı dozunda gavaj yoluyla verilirken dördüncü gruba kuersetin ile birlikte BPA verildi. Son enjeksiyon ve gavaj uygulamasından 2 gün sonra ratlar anestezi uygulanarak uterus dokuları ve kan örnekleri toplandı. Tek başına BPA uygulaması plazma MDA seviyesini artırırken total antioksidan kapasiteyi düşürdü (TCA). BPA uygulaması plazma oksitosin ve kortizol seviyelerinde ise konrol grubu ile karşılaştırıldığında bir değişime neden olmadı (P>0.05). Kuersetin ve BPA'nın birlikte uygulanması kontrol grubuyla karşılaştırıldığında MDA seviyesini düşürürken TCA, oksitosin ve kortizol seviyelerinde ise bir değişime neden olmadı (P>0.05). Kuersetin ve BPA'nın tek başlarına veya birlikte uygulanması oksitosin reseptör gen ekspresyonunun artmasına neden oldu (P

Bisfenol A Verilen Ratlarda Kuersetinin Kortizol ve Oksitosin Seviyeleri İle Oksitosin Reseptör Gen Ekspresyonu ve Uterus Morfometrisi Üzerine Etkileri

This study was carried out to evaluate effects of quercetin and bisphenol A (BPA), alone and in combination, on plasma cortisol and oxytocin levels, oxytocin receptor gene expression and morphometry of uterus in rat. After one week of acclimatization, twenty female rats were divided into four treatments as: control, received ethanol saline as injection intraperitoneally and gavaged hydroxypropylmethylcellulose (HPMC). The second group injected with 50 µg BPA per kg body weight dissolved in ethanol saline two times per week for 4 weeks. The third group gavaged 30 mg quercetin per kg body weight suspended in aqueous solution of HPMC two times per week for 4 weeks, while the fourth group was treated with quercetin along with BPA. Two days after final injection and gavage, rats were anesthetized and uterine and blood samples were collected. BPA alone increased plasma MDA level, decreased total antioxidant capacity (TCA) and had no effect (P>0.05) on oxytocin and cortisol levels in the plasma compared with control group. Quercetin along with BPA decreased MDA level, but had no effect (P>0.05) on TCA, oxytocin and cortisol levels compared to control. Quercetin and BPA, alone or in combination, resulted in increase (P<0.05) oxytocin receptor gene expression. Treatment with quercetin along with BPA increased (P<0.05) endometrium, but had no effect (P>0.05) on myometrium and epimetrium. It was indicated that quercetin may be a potential compound for reducing oxidative stress damages in uterus layer and increasing blood oxytocin level and its receptor in uterine, but cannot completely ameliorate the negative effects of BPA.

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