Complete Genome Sequence of a Novel Duck Parvovirus
Bu çalışmada, Fujian, Güneydoğu Çin'de Muscovy ördeklerinden türetilmiş yeni bir ördek parvovirus izolatlarını (NM100) sıralayıp tam genomlarını analiz ettik. Tam genom ve VP1 genine dayalı filogenetik analize göre, yeni ördek parvovirus suşu NM100'ün MDPV kümelerine ait olduğunu, oysa VP3 geni, NM100 suşunun GPV kümelerine ait olduğunu gösterdi. Benzerlik alanları analizi kullanılarak, iki olası genetik rekombinasyon olayları tespit edildi. Bu bulguların, doğada Muscovy ördek sürülerinde rekombinasyon yoluyla dolaşan yeni ördek parvovirusu olduğu değerlendirilmektedir ki, bunlar da su kuşu parvovirusunun moleküler özelliklerini ve evrimsel çeşitliliğini anlamamızı sağlar.
Çin'in Fujian Bölgesinde İzole Edilen Yeni Bir Ördek Parvovirus'unun Tüm Genom Sekansı
In the present study, we sequenced and analysed the complete genomes of a novel duck parvovirus (NM100) isolates derived from Muscovy ducks in Fujian, Southeast China. According to the phylogenetic analysis, based on the complete genome and VP1 gene showed that novel duck parvovirus strain NM100 belong to the MDPV clusters, whereas the VP3 gene showed that strain NM100 belong to the GPV clusters. Two putative genetic recombination events were detected using similarity plots analysis. These findings suggest that a novel duck parvovirus circulating in Muscovy duck flocks with recombination in nature, which enable us to understand the molecular characteristics and evolutionary diversity of waterfowl parvoviruses.
___
- Zádori Z, Stefancsik R, Rauch T, Kisary J: Analysis of the complete nucleotide sequences of goose and muscovy duck parvoviruses indicates common ancestral origin with adeno-associated virus 2. Virology, 212, 573, 1995.
- Shien J, Wang Y, Chen C, Shieh H, Hu C, Chang P: Identification of sequence changes in live attenuated goose parvovirus vaccine strains developed in Asia and Europe. Avian Pathol, 37, 499-505, 2008. DOI: 1080/03079450802356979
- Liu H, Wang H, Tian X, Zhang S, Zhou X, Qi K, Pan L: Complete genome sequence of goose parvovirus Y strain isolated from Muscovy ducks in China. Virus Genes, 48, 199-202, 2014. DOI: 10.1007/s11262-013- 4
- Wan C, Chen H, Fu Q, Fu G, Cheng L, Shi S, Huang Y, Hu K: Genomic characterization of goose parvovirus and Muscovy duck parvovirus co- infection in Fujian, China. Kafkas Univ Vet Fak Derg, 21, 923-928, 2015. DOI: 9775/kvfd.2015.13848
- Shao H, Lv Y, Ye J, Qian K, Jin W, Qin A: Isolation of a goose parvovirus from swan and its molecular characteristics. Acta Virol, 58,194-198, 2014.
- Chen H, Dou Y, Tang Y, Zhang Z, Zheng X, Niu X, Yang J, Yu X, Diao Y: Isolation and genomic characterization of a duck-origin GPV-related Parvovirus from Cherry Valley Ducklings in China. PLoS One, 10, e0140284, DOI: 10.1371/journal.pone.0140284
- Wan C, Huang Y, Shi S, Fu G, Cheng L, Chen H, Fu Q, Chen C, Hu K: Complete genome sequence of goose Parvovirus isolated from Anser cygnoides in China. Kafkas Univ Vet Fak Derg, 22, 155-158, 2016. DOI: 9775/kvfd.2015.14242
- Wan C, Chen H, Fu Q, Shi S, Fu G, Cheng L, Chen C, Huang Y, Hu K: Development of a restriction length polymorphism combined with direct PCR technique to differentiate goose and Muscovy duck parvoviruses. J Vet Med Sci, 78, 855-858, 2016. DOI: 10.1292/jvms.15-0326
- Wang J, Duan J, Meng X, Gong J, Jiang Z, Zhu G: Cloning of the genome of a goose parvovirus vaccine strain SYG61v and rescue of infectious virions from recombinant plasmid in embryonated goose eggs. J Virol Methods, 200, 41-46, 2014. DOI: 10.1016/j.jviromet.2014.02.014
- Chen S, Lin T, Hu Q, Li Y, Zhou W, Wu Z: Isolation and identification of a muscovy duckling parvovirus. Bing Du Xue Bao, 9, 228-235, 1993.
- Cheng Y, Hu Q, Li Y, Chen S: Selection of attenuated vaccine strain of muscovy duckling parvovirus and its biological characteristics. Chin J Vet Sci, 16, 118-121, 1996.
- Wang S, Cheng X, Chen S, Zhu X, Chen S, Lin F, Li Z: Genetic characterization of a potentially novel goose parvovirus circulating in Muscovy duck flocks in Fujian Province, China. J Vet Med Sci, 75, 1127- , 2013. DOI: 10.1292/jvms.12-0527
- Chen S, Hu Q, Cheng X, Li Y, Cheng Y, Lin T: Study on two-combinative attenuated vaccine of goose and muscovy duckling parvovirus. Chin J Vet Sci, 23, 226-228, 2003.
- Zhu Y, Zhou Z, Huang Y, Yu R, Dong S, Li Z, Zhang Y: Identification of a recombinant Muscovy Duck parvovirus (MDPV) in Shanghai, China. Vet Microbiol, 174, 560-564, 2014. DOI: 10.1016/j.vetmic.2014.10.032
- Zhao H, Xie Z, Xie L, Deng X, Xie Z, Luo S, Huang L, Huang J, ZengT: Molecular characterization of the full muscovy duck parvovirus, isolated in guangxi, china. Genome Announc, 2, e01249-14, 2014. DOI: 1128/genomeA.01249-14
- Martin DP, Biagini P, Lefeuvre P, Golden M, Roumagnac P, Varsani A: Recombination in eukaryotic single stranded DNA viruses. Viruses, , 1699-1738, 2011. DOI: 10.3390/v3091699
- Tyumentsev AI, Tikunova NV, Tikunov AY, Babkin IV: Recombination in the evolution of human bocavirus. Infect Genet Evol, 28, 11-14, 2014. DOI: 10.1016/j.meegid.2014.08.026
- Poonia B, Dunn PA, Lu H, Jarosinski KW, Schat KA: Isolation and molecular characterization of a new Muscovy duck parvovirus from Muscovy ducks in the USA. Avian Pathol, 35, 435-441, 2006. DOI: 1080/03079450601009563