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Expression of voltage-gated sodium channel in primary tumors and corresponding metastases in the copenhagen rat model of prostate cancer

Amaç: Artan miktardaki kanıt prostat kanser (PCa) hücrelerinin voltaj kapılı sodyum kanalları (VGSCs)’na sahipolduğunu ve bu kanalların hastalığın metastatik ilerlemesine katkıda bulunduğunu göstermektedir. Mevcut çalışmada, VGSC’lerin karsigonezde ve metastazdaki heterotopik ekspresyonunu araştırmak amaçlanmıştır.Güçlü metastatik sıçan Mat-LyLu PCa hücrelerinde in vit-ro olarak gözlemlenen VGSC ekspresyonunun, bu hücre- lerin sıçanlara subkuten enjekte edildiğinde primer tümör oluştuğunda ve hücreler akciğerlere metastaz yaptığındakorunup korunmadığı, değerlendirilmiştir. Yöntemler: PCa modeli Copenhagen ırkı erkek sıçanlarda güçlü metastatik Mat-LyLu hücrelerinin subkuten implantasyonu ile oluşturulmuştur. VGSC eksprsyonu bu modelde moleküler biyolojik ve immünokimyasal yöntemlerle araştırılmıştır. Bulgular: Ters transkripsiyon PCR ve immünohistokimya yöntemleri VGSC ekspresyonunun Mat-LyLu primer prostat tümörlerinde korunduğunu ve akciğer metatsazlarında da bu ekspresyonun devam ettiğini, mRNA ve protein düzeyinde göstermiştir. Sonuç: Sonuçlar, VGSC’lerin PCa’nin metastatik ilerlemesindeki baskın rolü ile uyumludur ve ‘kanserin ilerlemesinde VGSC’lerin rolü’ hipotezini tutarlı bir şekilde des- teklemektedir.

Prostat kanseri Copenhagen sıçan modelinin primer tümörleri ve metastazlarında voltaj kapılı sodyum kanal ekspresyonu

Objective: Increasing evidence indicates that prostatecancer (PCa) cells possess voltage-gated sodium chan-nels (VGSCs) and these channels contribute to the meta- static progression of the disease. In the present study, itwas aimed to investigate the heterotopic expression ofVGSCs in carcinogenesis and metastasis. It was evaluat-ed whether the expression of VGSC in strongly metastaticrat Mat-LyLu PCa cells observed in vitro is also conservedwhen these cells form primary tumors and metastasize tothe lungs upon their subcutaneous injection in rats.Methods: PCa model was induced in male Copenhagen rats by subcutaneous implantation of strongly metastatic Mat-LyLu cells. The expression of VGSCs was studied in the model utilizing molecular biological and immuno- chemical techniques. Results: Reverse transcription PCR and immunohisto- chemistry showed that the expression of VGSC is conserved in the primary prostate tumors of Mat-LyLu and not eliminated in the corresponding lung metastases at both the mRNA and protein level. Conclusion: The results are consistent with the predominant role of VGSC in the metastatic progression of PCa and consistently support the VGSC hypothesis of cancer progression.

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