The effect of blood withdrawal site on hematological values in mice
Bu çalışmada kan alma yerinin bazı hematolojik değişkenler üzerine etkilerinin araştırılması amaçlanmıştır. Bu amaçla toplam 21 Swiss albino dişi fare kullanılmıştır. Fareler üç gruba ayrılmışlar ve her grup farklı bir kan alma yeri için kullanılmıştır. Kan her hayvandan kalp, kuyruk veya retro-orbital pleksustan bir kez alınmıştır. PCV değerlerinin retro-orbital pleksusdan alınan kanda diğer iki yöntemle alınanlardakinden daha yüksek olduğu belirlenmiştir (P
Farelerde kan alma yerinin hematolojik değerler üzerine etkisi
In this study, the investigation of the effect of the blood withdrawal site on certain hematological values was aimed. For this purpose a total of 21 female Swiss albino mice were used. The mice were divided into three equal groups. Each group served for a different blood collection technique. Blood was withdrawn from the heart, the tail or from the retro-orbital plexus only for one time from each animal. The mean PCV value in blood gathered from retro-orbital plexus was higher than in blood collected from other two blood sampling sites (P
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- 1. Bellamy, G.J., Hinchliffe, R.F.: Venous and skin puncture blood counts compared. Clin. Lab. Hematol., 1988; 10: 329 - 334.
- 2. Berchelmann, M.L., Kalter, S.S., Britton, H.A.: Comparison of hematologic values from peripheral blood of the ear and venous blood of infant baboons (Papio cynocephalus). Lab. Anim. Sci., 1973; 23: 48 - 52.
- 3. Coles, E.H.: Veterinary clinical pathology. Fourth Ed. W.B. Saunders Company Philadelphia, 1986, 10 -72.
- 4. Diehl, K.H., Hull, R., Morton, D., Ptisfer, R., Rabemampianina, Y., Smith, D.,Vidal, J.M., van de Vorstenbosch C.: A good practice guide to the administration of substances and removal of blood, including routes and volumes. J. Appl.Toxicol., 2001; 21: 15-23.
- 5. Doeing, D.C., Borowicz, J.L., Crockett, E.T.: Gender dimorphism in differential peripheral blood leukocyte counts in mice using cardiac, tail, foot and saphenous vein puncture methods. BMC Clinical Pathology, 2003,3:1-6.
- 6. Dougherty, T.F., White. A.: An evaluation of alterations produced in lymphoid tissue by pituitary - adrenal cortical secretion. J. Lab. Med., 1947; 32: 584 - 605.
- 7. Gerstner, R., Gordon, A.S.: Effects of withdrawal of growth hormone and cortisone treatment on the blood picture of hypophysectomized rats. Am. J. Physiol.,1958; 194: 485 - 487.
- 8. Jain, N.C.: Examination of the Blood and Bone Marrow. In: Essentials of the Veterinary Hematology. Philadelphia, Lea & Febiger, 1986, 19-53.
- 9. Komiya, E.: Die zentralnervöse Regülation des Blutes. Georg Time Verlag, Stuttgart. 1955.
- 10. Nemzek, J.A., Bolgos, G.L., Williams, B.A., Remick, D.G.: Differences in normal values for murine white blood cell counts and other hematological parameters based on sampling site. Inflamm. Res., 2001; 50: 523 - 527.
- 11. Nichols, J., Miller Jr A.T.: A comparison of the total leukocyte count in the hearth blood and peripheral blood of the rat. Science, 1948; 108: 378 - 379.
- 12. Pfeil, R.: Effects of repeated blood samplings on locomotor activity, evasion and wheel - running activity in mice. Lab. Anim., 1988; 22: 46 - 50.
- 13. Piette, C., Piette, M.: Iode hormonal sérique, éosinophiles et basophiles sanguins. Path. - Biol., 1973; 21: 405 - 410.
- 14. Schenell, M.A., Hardy, C., Hawley, M., Proper, K.J., Wilson, J.M.: Effect of blood collection technique in mice on clinical pathology parameters. Human Gene Therapy, 2002; 13: 155 - 162.
- 15. Schermer, S.: Die Blutmorphologie der Laboratoriumstiere. 1. Auflage. Barth Verlag, Leipzig., 1954.
- 16. Scheufler, H.: Über den Einfluß des Ortes und der art der Blutentnahme auf das Blutbild der Hausmaus (Mus musculus domesticus, RUTTY 1772). Zscr Versuchstierk, 1967; 9: 152 - 157.
- 17. Weigent, D.A., Blalock, J.E.: Interactions between the neuroendocrine and immune system. Common hormones and receptors. Immunol. Rev., 1987; 100: 79 - 108.
- 18. Yang, Z.W., Yang, S.H., Chen, L., Qu, J., Zhu, J., Tang, Z.: Comparison of blood counts in venous, fingertip and arterial blood and their measurement variation. Clin. Lab. Haem., 2001; 23: 155 - 159.