Muharrem BALKAYA, Hümeyra ÇELER, Hüseyin VOYVODA, Cengiz ÜNSAL

Some hematological and biochemical characteristics of male and female Sprague-Dawley rats

Bu çalışma yaklaşık 20 yıldır Türkiye'de ye iki senedir de laboratuvar hayvanlarıünitemizde relatif olarak kapalı bir koloni şeklinde yetiştirilen erkek ve dişi Sprague-Dawley ratlarında bazıhematolojik ve serum biyokimyasal parametrelerin biyolojik normlarının belirlenmesi amacıyla gerçekleştirildi. Eritrosit, lökosit ve eozinofil sayımlarında standard yöntemler kullanıldı. Hematokrit, mikrohematokrit yöntemle saptandı. Diferensiyal kan tablosu, taze hazırlanmış ve Pappenheim'm panoptik yöntemine göre boyanmış olan iki frotiden toplam 500 hücrenin değerlendirilmesi ile yapıldı. Serum alkalen fosfataz aktivitesi, total protein, kalsiyum, fosfor, magnezyum ve kolesterol değerlerinin belirlenmesinde ticari killer kullanıldı. Ortalamaların karşılaştırılmasında bağımsız gruplar için t-Testi ve Man Whitney-U testi kullanıldı. Hematokrit değer ve relatif lenfosit oranıdışında, araştırılan diğer parametreler açısından erkek ve dişi ratlar arasında önemli bir fark bulunmadı. Hematokrit değe'r erkeklerde, relatif lenfosit oramise dişilerde daha yüksek bulundu (p

Erkek ve dişi Sprague-Dawley ratların bazı hematolojik ve biyokimyasal parametreleri

This study was carried out to determine the biological norms of some hematological and serum biochemical variables of the male and female Sprague-Dawley rats, which have been bred since ca. two decades in Türkiye, and also since two years in our Laboratory Animal Unit as a relatively closed stock colony. Erythrocyte, leukocyte and eosinophil counts were made by standard methods using Turk's solution in improved Neubauer hemocytometry. Packed cell volume (PCV) was determined with microhematocrit method, and leukocyte differentials were carried out on two slides for which a total of 500 leukocytes were considered (2x250). Serum biochemical variables were determined by commercial kits according to the proposal of the producer. In serum; total protein level, alkaline phosphatase activity and, calcium, phosphorus, magnesium and cholesterol levels were determined. The results were statistically analysed for which Man Whitney-U test and t-Test for independent groups were used to compare the means. That is because there was no difference in results of these two tests, t-Test results were considered.Except the packed cell volume (PCV) value and relative lymphocyte rate, there was no difference between male and female animals for all blood constituents studied. The males had a higher mean PCV value than the females (p

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