Köpek spermasının pellet ve payet yöntemine göre tris ve süt sulandırıcıları ile dondurulması

Bu çalışmada, sulandırıcı komponeııtleri ve dondurma tekniklerinin köpek sperma-tozoa morfolojisi üzerindeki etkisinin tespiti amaçlandı. Çalışmada, dört farklı köpek ırkından parmak maniplasyonu yardımıyla sperma alındı. Sulandırıcı olarak %4 gliserollü süt-glikoz sulandırıcısı ve %4 gliserollü Tris-sitrik asit-fnıktoz sulandırıcısı kullanıldı. Sperma alınıp split sample yöntemine göre iki kısma ayrıldıktan sonra süt ve Tris sulandırıcısı ile sulandırıldı ve ekilibrasyon sonrası iki eşit hacime ayrılarak her bir hacim pellet ve payet metoduna uygun olarak donduruldu. Alınan ejakulatlarda ortalama %87.25±3.79 motilite ve %6.20±3.31 akrozom, %3.80± 1.90 baş, %3.90±3.64 orta kısım, %8.90±6.12 kuyruk olmak üzere toplam %22.80±7.33 morfolo-jik bozukluk saptandı. Ekilibrasyon sonrası süt sulandırıcısında, %67.50±8.02 motilite ve %10.45±4.72 akrozom, %6.30±3.59 baş, %3.20±3.73 orta kısım, %8.85±3.06 kuyruk olmak üzere toplam %28.80±6.22 morfolojik bozukluk saptanırken, Tris sulandırıcısında %76.50±5.15 motilite ve %10.35±4.00 akrozom, %4.45±2.45 baş, %2.85±2.62 orta kısım, 9.15±5.11 kuyruk olmak üzere toplam %26.80±4.65 morfolojik bozukluk saptandı. Ekilibrasyon sonrası her iki sulandırıcıda saptanan morfolojik bozukluklar benzer olurken, Tris sulandırıcısmdaki motilite oranı istatistiksel açıdan yük^k bulundu (p

Freezing of dog semen according in pellets and paillettes with tris and milk extenders

In this study the effects of extender components and freezing tecniques on the morphology of dog semen were investigated. Semen was collected from 4 different dog breeds by digital manipulation. The extenders used were, 4% glycerol containing milk-glucose and 4% glycerol containing Tris-citric acid-fructose extenders. After collecting, semen was divided into two by split sample procedure and extended with milk and Tris extenders. These aliquots were divided into two after equilibration and each volume was frozen in pellets and straws. The mean spermatological values of the ejaculates were 87.25±3.79% motility and 6.20±3.31% acrosome defect, 3.80±1.90% head defect, 3.90±3.64% mid-piece defect, 8.90±6.12% tail defect and total 22.80±7.33% morphologic-defect rate. After equilibration 67.50±8.02% motility and 10.45±4.72% acrosome defects, 6.30±3.59% head defects, 3.20±3.73% mid-piece defects, 8.85±3.06% tail defects and total 28.80±6.22% total defect rate was determined in the milk extender. In Tris extender the above values were 76.50±5.15%, 10.35±4.00%, 4.45±2.45%, 2.85±2.62%, 9.15±5.11% and 26.80±4.65% respectively. Morphologic defect rates of both extenders were similar after equilibration but motility rates of the Tris extender was higher statistically (p

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