The effectiveness of oregano oil as a surface disinfestation agent in plant tissue culture

Bu çalışmada kekik yağının (Origanum vulgare L.), kalanşo (Kalanchoe bossfeldiana Poelln.), afrika menekşesi (Saintpaulia ionantha), paşakılıcı (Sansevieria trifasciata Prain.), gül (Rosa hybrida L. 'Savalav'), begonya (Begonia x erythrophylla 'Beefsteak'), türk kekiği (Origanum onites L.), ve yunan kekiği (Origanum hirtum L.) bitkilerinin yüzey dezenfeksiyonları üzerine etkisi değerlendirilmiştir. Sırasıyla 0.4, 0.5 ve 1 g $l^{-1}$ konsantrasyonundaki kekik yağı çözeltisi içerisinde gül, afrika menekşesi, ve kalanşo eksplantlarının yüzey sterilizasyonları yapılarak doku kültürü test tüplerine transfer edilmiştir. Kullanılan dozlardaki kekik yağı, kültürlerdeki kontaminasyonunun önlenmesi üzerine etkili olmuştur. Aynı zamanda 1,3, and 5 g $l^{-1}$ kekik yağı veya %10 çamaşır suyu (%0.525 NaOCl) ile eksplant yüzey sterilizasyonu yapılan paşakılıcı, türk ve yunan kekiklerinin kültürlerinde de bir bulaşma ortaya çıkmamıştır.

Bitki doku kiltüründe eksplant yüzey dezenefaktant maddesi olarak kekik yağının etkileri

The effect of oregano oil {Origanum vulgare L.) on surface sterilization of kalanchoe (Kalanchoe bossfeldiana Poelln.), african violet (Saintpaulia ionanthd), snake plant (Sansevieria trifasciata Prain.), miniature rose (Rosa hybrida L. 'Savalav'), begonia (Begonia x erythrophylla 'Beefsteak'), and seeds of Turkish oregano (Origanum onites L.), Greek oregano (Origanum hirtum L.) was evaluated. The oregano oil at 0.4, 0.5 and 1 g $l^{-1}$ was effective in eliminating contamination in miniature rose, African violet and kalanchoe, respectively. Also all the oregano oil concentrations tested (1, 3, and 5 g $l^{-1}$ ) or 10 % Clorox (0.525 % NaOCl) eliminated contamination in snake plant, and seeds of Turkish oregano, or Greek oregano.

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