Sıçanlarda, Subhipnotik Doz Kronik Desfluran Maruziyetinde, DNA Hasarının Araştırılması

Amaç: Bu çalışmada, subhipnotik dozda, kronik desfluran gazına maruz kalan sıçanlardaki DNA hasarının araştırılması amaçlandı. Materyal ve metod: Deneysel çalışma, Harran Üniversitesi Tıp Fakültesi Etik Kurulu izni sonrasında aynı fakültenin Farmakoloji Laboratuarında yapıldı. Çalışma için 200-230gr ağırlığında 18 adet dişi Wistar türü albino sıçan kullanıldı. Sıçanların çalışma öncesi ve sonrasındaki beslenme ve bakımları, Harran Üniversitesi Deney Hayvanları Laboratuarında yapıldı. Sıçanlar randomize 2 gruba ayrıldı (Grup I=Desfluran, Grup II= Kontrol). Anestezik gaz verme sistemi için, özel olarak tasarlanmış plastik kaplar kullanıldı. Grup I' de sıçanlara, Datex-Ohmeda Tec6 Plus vaporizatörü ile hipnoz oluşturmayacak dozlarda (%0.5-1) desfluran verildi. Grup II'de de aynı düzenek kullanıldı, ancak desfluran vaporizatörü kapalı tutuldu. Her iki grupta deney düzeneğine %50 oksijen-hava karışımı 5 litre /dakika taze gaz akışı hızında verildi. Ortamdaki desfluran ve oksijen konsantrasyonları bir anestezik gaz monitörü (Criticare ®) ile sürekli olarak ölçüldü. Çalışma gazlarına maruz kalma durumu 5 gün boyunca günde 1 saat olacak şekilde tekrarlandı. Deney dışındaki saatlerde sıçanlar kendi kafeslerinde oda şartlarında bakıldılar. Beşinci günün sonunda 50 mg/kg intraperitoneal pentothal ile anestezisi sağlanan sıçanlar, supin pozisyonunda ekstremitelerden masaya tespit edildi. Sıçanlardan intrakardiak girişimle kan örnekleri alındı. Alınan kanlar heparinli tüplere aktarılarak özel bir kap içerisinde, soğuk ortamda, en kısa sürede Harran Üniversitesi Tıp Fakültesi Biyokimya Anabilim Dalı Laboratuarına ulaştırıldı. Deney hayvanları kan alındıktan sonra sakrifiye edilip, üzerlerine kireç kaymağı dökülüp toprağa gömüldüler. Alınan kanlardaki mononükleer lökositlerinden Comet Assey yöntemi ile DNAhasarları ölçüldü. Ayrıca aynı kan örneklerinden, antioksidan kapasite ve oksidatif stress indeks değerleri analiz edildi. Bulgular: Deneysel çalışma sırasında hiçbir sıçanda genel anestezi durumu oluşmazken, tüm sıçanlar 5 gün boyunca normal aktivitelerini sürdürdüler. Kan örneklerinden çalışılan DNAhasarı, antioksidan kapasite ve oksidatif stres indeks değerleri, her iki grupta benzer bulundu.Sonuç: Kronik olarak desflurana maruz kalan sıçanlarda, kontrol grubuna göre, belirgin bir DNA hasarının oluşmadığı sonucuna varıldı.

Anahtar Kelimeler:

Desfluran, kronik maruziyet, DNAhasarı

Investigation of DNA Damage in Rats in Chronically Exposed to Sub-Hypnotic Dose Desflurane

Background: In this study the aim was to research the DNA damage in rats whose chronic desfluran gases exposed. Metods: This experimental study, after the Ethic Committe permission was made in Harran University Medical Faculty Farmacology Department. For the study 200-230 gr 18 female albino wistar rats were used. After and before the study care and the feeding of the rats were made in the Harran University experimental animal laboratory. Rats divided in two groups (Group I: Desfluran, Grup II: Control ). For the anaesthesia pump system we used special planned plastic plate. Datex Ohmeda Tec 6 plus vaporizator was used to Group I rats to whom hypnotic doses desflurane was given ( % 0,5-1 ). İn the second group same mechanism was used but the desflurane vaporizator was closed. Each group %50 O2 and desflurane gas mixture was given in the 5lt/minute speed. In the environment the desflurane and the O2 concentration were measured continously by the anaesthesia gases monitor ( criticare ). The condition of exposing to the gases, again 1 hour in 5 days. Outside of experiment, rats waited in room condition and in their cages. At the end of fifth day, 50mg/kg with penthotal sodium, general anaesthesia was made and their extremities were fixed in the supine position fixing to the table. Blood samples were taken from intracardiac undertaking. The blood samples were taken heparinized tubes and put in cold condition in the special plate. Then sent to the Harran University Medical Faculty Biochemical Department. The experimental rats sacrificed and then burried with lime. DNAdamage was measured in taken bloods mononuclear leukocytes with Comet Assay method. İn addition, in same blood samples were measured antioxidant capacity and oxidative stress. Results: During the study, general anaesthesia conditions in any rat were not seen and rats were continued their normal activity. DNAdamage, antioxidant capacity and oxidative stress were similar in both groups. Conclusions: It was concluded that not occur in a significant DNA damage, compared to the control group in rats exposed chronically desflurane.

Keywords:

Desflurane, chronic exposure, DNAdamage,

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