Hasan PINAR, Mehmet YAMAN, Hasan Cumhur SARISU, Aydın UZUN, Merve Arefe YİĞİT

Bazı Kiraz Anaçlarının Genetik Akrabalık İlişkilerinin RAPD Moleküler Markır Yöntemi İle Belirlenmesi

Bu çalışmada kiraza anaç olarak kullanılan ve/veya kullanılacak olan bazı genotiplerin RAPD moleküler markır yöntemi kullanılarak genetik akrabalıklarının belirlenmesi amaçlanmıştır. Yirmi farklı kiraz anacının (9 adeti kuş kirazı) genetik benzerliklerinin belirlemesinde 16 farklı RAPD primerinin kullanıldığı çalışmada, 16 primerden 92’si polimorfik olmak üzere 109 bant elde edilmiş ve ortalama polimorfizm oranı %83.73 olarak belirlenmiştir. Elde edilen bulgulara göre kiraz anacı genotipleri arasındaki genetik benzerlik 0.60-0.95 arasında değişmiş olup en düşük benzerlik % 60 ile SL64 genotipi ile diğer tipler arasında, en yüksek benzerlik oranı ise %95 ile Kuş Kirazı-3 ve Kuş Kirazı-4 genotiplerinden elde edilmiştir. Elde edilen dendograma göre üç ana grup oluşmuş olup, birinci grup Kuş Kirazı-2 (KK2), Kuş Kirazı-6 (KK6), Kuş Kirazı-7 (KK7) ve Kuş Kirazı-8 (KK8) yer almıştır. İkinci grupta Kuş Kirazı-3 (KK3), Kuş Kirazı-4 (KK4), Kuş Kirazı-5 (KK5), Kuş Kirazı-9 (KK9), Kuş Kirazı-10 (KK10), İdris ve diğer yabancı anaçlar yer almıştır. Üçüncü grupta sadece SL64 genotipi yer almıştır. Elde edilen bulgular RAPD moleküler markır tekniğinin kiraz anaçları arasındaki genetik ilişkilerin belirlenmesinde etkin bir şekilde kullanılabileceğini göstermektedir.

Anahtar Kelimeler:

Prunus avium L., Kiraz, Anaç, Genetik çeşitlilik, RAPD

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