Niloofar FAHIM KHOSHBAKHT, Javad FEIZY, Somayeh Sadat JANATI FAKOOR, Hamed Reza BEHESHTI

LC determination of natamycin in doogh with UV detection

Doogh içinde natamisin belirlenmesi için Yüksek Performans Sıvı Kromatografi yöntemi (HPLC-UV) ile basit ve hızlı bir yöntem, ilk olarak geliştirilmiştir. Doogh İran'a özgü yoğurt, su ve nane ile yapılan serinletici bir içecektir. Yöntem, 10 g Doogh örneğinin 75 mL metanol ve 25 mL saf su ile ekstraksiyonu esasına dayanmaktadır. 20 μL ekstrakt, filtre edildikten sonra HPLC'ye enjekte edilmiştir. Natamisin, Grace Smart RP C18 kolonunda (100 x 4.6 mm, 3 μm I.D.) belirlenmiştir ve mobil faz asetonitril:30 mM perklorik asittir (35:65). Süre 5 dakikadır. Akış hızı 0.5 mL/ dakikadır ve analiz 304 nm'de gerçekleştirilmiştir.

HPLC-UV ile Doogh'da natamisin belirlenmesi

A rapid and simple High-Performance Liquid Chromatographic method with UV detection (HPLC-UV) has been developed for the first time to determination of natamycin in Doogh. Doogh is a refreshing drink that is made with yogurt, water, and mint. The method is based on extraction of 10 g of Doogh with 75 mL of methanol and 25 mL of distilled water. An aliquot of 20 μl of Extract after filtration injected to HPLC. This compound was detected using a Grace Smart RP C18 column (100 x 4.6 mm, 3 μm I.D.) and the mobile phase used, was acetonitril: 30mM perchloric acid (35:65) in 5 min. The flow rate was maintained at 0.5 mL/min and the analytical signal was measured at 304 nm.

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