Mehmet Berk TORUN, HASAN CÜCE, Aydan CANBİLEN

Uzun süreyle oral çinko sülfat kullanılan yaşlı farelerde timusta meydana gelen histolojik değişikliklerin ışık mikroskobu ile araştırılması

Amaç: Normal diyetle beslenen farelere ilave oral çinko verilmesiyle timusta meydana gelecek morfolojik değişikliklerin araştırılması amaçlanmıştır. Yöntem: 18 aylık, yaşlı Balb/c farelerine (n=17) iki ay süreyle içme sularının içinde 10 mg/kg çinko sülfat verilmiş ve normal miktarda çinko içeren diyetle beslenen kontrol grubu ile (n=17) karşılaştırılmıştır. Timusların ağırlık ve hacimleri ölçüldükten sonra histolojik yapıları ışık mikroskobunda incelenmiştir. Bulgular: Sekiz hafta sonunda deney grubundaki farelerin timuslarında kontrol grubuna göre hacim ve ağırlık artışı tespit edilmiştir. Timuslardaki ağırlık artışı anlamlı değildir, timuslardaki hacim artışı anlamlıdır (P= 0.022). Timusun histolojik incelemesinde kontrol grubuna göre belirgin farklılıklar yoktur ve bağ doku oluşumu görülmemiştir. Sonuç: Oral çinko farelerde timus boyutunda belirgin, homojen bir büyüme oluşturmakla beraber deney ve kontrol grupları arasında belirgin bir histolojik farklılık ortaya çıkmamıştır.

Anahtar Kelimeler:

Çinko, Modeller, hayvan, Fare, Histoloji, Yaşlı, Mikroskopi, polarizasyon, Timus bezi

The light microscobic changes of the thymuses of the old mice using long time oral zinc sulphate

Objective: The aim of the study was to see the morphologic changes of thymuses of mice, by adding extra zinc to their normal diet. Methods: We supplied zinc sulphate (10 mg/kg) to the 18 months old Balb/c mice (n=17) in tap water in addition to their normal diet and compared them with a control group (n=17) which fed with normal diet. After the determination of weights and volumes, thymuses are examined histologically by light microscobe. Results: After eight weeks, the extra zinc supplemented mice thymuses increased by weight and volume than the control group. The increases in thymus volumes were significant (P=0,022) whereas the increases of thymus weights were not. The histological sections of thymuses had normal appearance without any connective tissue grow and showed no significant differences between the control group and the experimental group Conclusion: Oral zinc supplementation has possitive effects on increasing the thymus dimensions homogenously, but no significant histologic differences were present between the experimental and control groups.

Keywords:

Zinc, Models, Animal, Mice, Histology, Aged, Microscopy, Polarization, Thymus Gland,

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