Arctium minus (Hill.) Bernh. tohumlarında gibberellik asit uygulamasının çimlenme ve alfa-amilaz enzim aktivitesi üzerine etkisi

Arctium minus (Hill) Bernh. (Asteraceae) tohumlarına 10,100 ve 1000 ppm gibberellik asit (GA3) uygulaması yapılmış ve GA3'ün tohum çimlenmesi ve a - amilaz enzim aktivitesi üzerindeki etkileri incelenmiştir. Her üç konsantrasyonda çimlenme yüzdesi ve enzim aktivitesi kontrole göre daha fazla olmuş, fakat 10 ve 100 ppm'lik konsantrasyonlarda çimlenme oram % 100 iken, 1000 ppm'lik konsantrasyonda % 60 olmuştur. Yine a - amilaz enzim aktivitesi de düşük konsantrasyonlarda daha yüksek olarak saptanmıştır.

The effect of gibberellic acid alpha-amylase activity and germination of Arctium minus (Hill.) Bernh. seeds

Arctium minus (Hill) Bernh. (Asteraceae) seeds were treated with 10, 100, 1000 ppm gibberellik asit (GA3) and effect of GA3 on the germination and a -amylase activity has been investigated. Germination rates and a - amylase enzyme activity were higher than control in all three concentrations. But 1000 ppm GA3 reduced germination rate and a - amylase activity than others. However, all three concentrations promoted germination and induced a- amylase enzyme activity.

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1-Miyata, S. and Akazawa, T. "Biosynthesis of - amylase iri relation to Protein Glycosylation", Plant Physiol., 70: 147-153, (1982).
2-Bewley, J.D., and Black, M. "Seeds-physiology of development and germination" 367 pp. Plenum Press. New york, (1985).
3-Fincher, G.B. "Molecular and cellular biology associated with endosperm mobilization in germination cereal grains", Ann. Rew. of Plant Physiol. and Plant mol. Biol., 40:305-346, (1989).
4-Thomas, T.H. "Relationship between bolting-resistance and seed dormancy of different celery cultivars", Sci. Hortic, 90:311-316, (1978).
5-Drewes, F.E. and van Staden J. "Germination of Tagetes minuta L. II. The role of gibberellins". Physiol. Plant growth Regul. 9:285-291, (1990).
6-Drewes, F.E. and van Staden, J. "Reserve mobilization during germination of Tagetes munita L. " Ann. of Botany, 68:79-83, (1991):
7-Thomson, P.A. "Germination of species of Labiatae in response to Gibberellins",Physiol.Pplantarum, 22:575-586,(1969).
8-Karssen, CM., Zagorski, S., Kepczynski, J., and Groot, S.P.C. "Key role for endogenous Gibberellins in the control of seed germination" Ann. of Botany. 63:71-80, (1989).
9-Durrant, M.J., and Mash, S.J. "Sugar-beet seed steep treatments to improve germination under cold, wet conditions" Plant growth Regul., 10:45-55, (1991).
10.Sanwo, M.M., and De mason, D.A. "Gibberellic acid (GA3) induced enhancement of oc-amylase activity in the aleurone of Shrunken-2 maize kernels", Amer.J. of Botany, 8:987-996,(1994)
11-Mahajan, A. And Dua, S. "Effect of hormones on germination of lin seed (Linum usitatissimum L.)'',J. of Plant Sci. Research., 3: 121-128, (1987).
12-Sun, 2., and Henson, C.A. "A quantitative assesement of the importance of barley seed oc-amylase, b-amylase debranching enzyme and oc- glikozidase in starch degratation", Arch. Biochem. Biophys., 284:299-305, (1991).
13.Hilhorst, W.M. and Karssen, Ç.M. "Dual effect of light on the gibberellin and nitrate stimulated seed germination of Sisymbrium officinale L. And Arabidopsis thaliano L", Plant Physiol., 86:591-597, (1988)
14-Bewley, J.D. and Black, M. "Physiology and Biochemistry of seeds" vol. I. pp.3 Berlin Heidelberg, Mew york, (1983).
15-Rinderknecht, H., Wilding, P. And Haverbord, B.J. "A new method for the determination of oc-amylase", Experientia, 23:805,(1967).
16-Lowry, O.H; Rosenbrough; N.S., Farr, A.L. and Randall, R.J. "Protein measurement with folin-fenol reagent", J. Biol. Chern;, 193:365, (1951).
17-Koyama, K., Wada, A., Maki, Y., and Tanaka, A. "Changes in the protein composition of cytoplasmic ribosomes during the greening of etiolated barley leaves", Physiol. Plantarum, 96:85-90, (1996)
18-Yazgan, M. "The effect of salinity on the endogenous Gibberellins and cytokinins levels of wheat grains during Germination", E.Ü.J.of Sci.Faculty, series B.vol VIII NR.1 pp. 11-19,(1986)
19-Grubusic, D. Kanjevic, R., and Neskovic, M "The effect of some growth regulators on light-induced germination of seeds", Physi. Plantarum, 72:525-528, (1988).
20-Grubusic, D. Neskovic, M., and Kanjevic, R. "Changes in light sensitivity of Paulownia tomentosa and P. fortunei _seeds", Plant Sci., 39:13-16, (1985).
21-Dennis, F.G., Martin G.C., Gaskin, P. And MacMillan, J. "Gibberellins in mature Apple seeds - contaminants", Planta, 147:376-377,(1980)
22-Kovac, M., and Kregor, I. "Protein metabolizm in silver during germination",Plant Physiol. Biochem., 27(1). 35-41,(1989)
23-MacGregor, A.W. Marchylo B.A. and Kruger, J.E. "Multiple oc-amylase, components in germinated cereal grains determinated isoelectric focusing", Cereal Chem,65:326-333, (1988).
24-Iwuoha, C.I., and Aina J.O. "Effects of steeping condition and germination time on the oc-amylase activity, phenolics content and malting loss of Nigerian local reol and hybrid short Kauro Sorghum malts", Food Chem., 58(4) 289-295, (1997)