Levent OKTAR, Metin ALKAN, Mustafa ARSLAN, Ayşegül KÜÇÜK, Şaban Cem SEZEN, Yusuf ÜNAL, Barış MARDİN, Yiğit KILIÇ, Abdullah ÖZER

Ratlarda Levosimendan ve Nigella Sativa'nın Miyokardiyal İskemi Reperfüzyon Hasarına Etkilerinin Değerlendirilmesi

Giriş: İskemi-reperfüzyon hasarı, doku iskemisi tarafınca meydana gelen bir olaylar zinciridir. Hücre hasarını takiben reperfüzyon, aktif bir inflamatuar yanıta neden olur. Araştırmamızda, ratlarda Levosimendan ve Nigella Sativa'nın miyokardiyal iskemi-reperfüzyon hasarı üzerindeki koruyucu etkisini değerlendirmeyi amaçladık. Yöntem: Araştırmamıza yirmi dört adet Wistar albino rat dahil ettik. Ratlar rastgele dört deney grubuna ayrıldı. Grup K'daki (kontrol grubu) ratların koroner arterleri oklude veya reperfüze edilmedi. Sol ön inen koroner arter miyokardiyal İR yapmak için 30 dakika bağlandı ve daha sonra İR (İR), İRLevosimendan (24ug / kg) (İRL) ve İR-Nigella Sativa (0.2 mL / kg) (İRNS) gruplarında 2 saat reperfüze edildi . Bulgular: İnflamasyon bulguları İR grubunda C, İR-NS ve İR-L gruplarına göre anlamlı olarak yüksek bulundu (p=0.001, p=0.019, p=0.019, sırasıyla). K, İR-NS ve İR-L grupları ile karşılaştırıldığında, mikroskobik miyokardiyal disorganizasyon, İR grubunda anlamlı olarak daha yüksekti (p

Assessment of the Effects of Levosimendan and Nigella Sativa on Myocardial Ischemia Reperfusion Injury in Rats

Objective: Ischemia-reperfusion injury is a chain of events put in place bytissue ischemia. Reperfusion following the damage of cell causes an activeinflammatory response. In our research we tried to evaluate the protectiveeffect of Levosimendan and Nigella Sativa on myocardial ischemia-reperfusioninjury in rats.Methods: We included twenty-four Wistar albino rats in our research. The ratswere randomly divided into four experimental groups. The coronary arteriesof rats in Group C (control group) were not occluded or reperfused. Leftanterior descending coronary artery was ligated for 30 min to performmyocardial IR and then reperfused for 2 h in the IR (IR), IR-Levosimendan(24µg/kg) (IRL) and IR-Nigella Sativa (0.2 mL/kg) (IRNS) group.Results: Inflammation findings were significantly higher in the IR groupcompared with the C, IR-NS, and IR-L groups (p=0.001, p=0.019, p=0.019,respectively). Compared with the C, IR-NS, and IR-L groups, the microscopicmyocardial disorganization was significantly higher among the IR group(p

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