Didem PEKMEZCİ, Yücel MERAL, Güvenç GÖKALP, E. Emek ONUK, Duygu ÇAKIROĞLU

Yeni doğan buzağılarda çeşitli Hematolojik ve Biyokimyasal parametreler İle kolostral immun globulinler arasındaki ilişkinin belirlenmesi

Bu çalışmada yeni doğan buzağılarda kolostrum alımını takiben serum immunoglobulin seviyeleri ile serum γ-glutamyltransferase (GGT) seviyesi arasındaki ilişki araştırılmıştır. Bu amaçla 30 adet yeni doğan buzağıdan, doğumu takiben ve 24 saat sonra, kan örnekleri alınarak, kan sayımı yapılmış ayrıca total protein, serum GGT ve serum alkaline fosfataz (AP) ve Kuantitatif ELISA yöntemiyle total immunoglobulin G ve total immunoglobulin M oranları belirlenmiştir. Alkalen fosfataz (AP) ve Total protein düzeyleri açısından iki örnekleme arasında herhangi bir farklılık saptanmazken, buzağıların immunoglobulin M ve G düzeyleri ile serum GGT düzeylerinde ise 0. gün ve 1. günler arasında istatistiki olarak önemli bir farklılık gözlenmiştir.

Demonstration of relationship between variative Haematological and Biochemical parameters and immunoglobuline of colostrum in newborn calves

In this study, relationship between the γ-glutamyltransferase (GGT) and immunoglobuline levels of newborn calves was investigated. Blood samples was obtained from 30 calves following birth and 24 hours after birth. Total blood counts were performed. In addition to this, detections of total protein, serum GGT and serum alkaline phosphatase (AP) and also determination of total immunoglobuline G and total immunoglobuline M levels were performed by quantitative ELISA method. A significant difference could not be observed regarding to AP and total protein levels. However, significant differences were observed in 0 day and 1st day with regard to immunoglobuline M and G, and serum GGT levels. In conclusion, according to data of this study we could say that serum GGT level can be accepted as an indicator of immunoglobuline absorbtion in neonatal calves.

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1. Arguello A, Castro N, Capote J. Evaluation of a color method for testing immunoglobulin G concentration in goat colostrum. J Dairy Sci 2005; 88(5): 1752- 1754.
2. Abel Francisco SF, Quigley JD. Serum immunoglobulin concentrations after feeding maternal colostrum or maternal colostrum plus colostral supplement to dairy calves. Am J Vet Res 1993; 54(7): 1051-1054.
3. Zarrili A, Micera E, Lacarpia N et al. Evaluation of ewe colostrum quality by estimation of enzyme activity levels. Revue Med Vet 2003;154(8-9): 521- 523.
4. Courtney AK, Epperson WB, Wittig TA, et al. Defining failure of passive transfer in South Dakota beef calves. AES 113th Annual Report, 2000.
5. Hopkins FM, Dean DF, Greene W. Failure of passive transfer in calves: comparasion of field diagnosis methods. Modern Veterinary Practice 1984; 625-628.
6. Weaver DM, Tyler FW, Vanmetre DC, et al. Passive transfer of colostral immunoglobulins in calves. J Vet Intern Med 2000; 14: 569-577.
7. Basoglu A, Camkerten I, Sevinc M. Serum Immunoglobulin concentrations ın diarrheic calves and their measurement by single radial immunodiffusion. Israel Vet Med Assoc 1999; 54(1): 9-10.
8. Quigley JD. Using the colostrometer to measure colostrum quality. http://www.calfnotes.com/pdffiles /CN022.pdf 2001/15.10.2009.
9. Wesselink R, Stafford KJ, Mellor DJ, et al. Colostrum intake by dairy calves. NZ Vet J 1999; 47: 31-34.
10. John, P.W.M. Statistical design and analysis of experiments (Classics in Applied Mathematics No 22.) Publisher: Society for Industrial Mathematics, 1999.
11. Knowles TG, Edwards JE, Bazeley KJ, et al. Changes in the blood biochemical and haematological profile of neonatal calves with age. Veterinary Record 2000; 147: 593-598.
12. Zanker IA, Hammon HM, Blum JW. Dealyed feeding of first colostrums: are there prolonged effects on haematological, metabolic and endocrine parameters and on growth performance in calves? J Anim Physiol and Anim Nut 2001; 53-66.
13. Turgut K. Veteriner Klinik Laboratuar Teşhis. Bahçıvanlar Basım Sanayi, 2000.
14. Morin DE, Constable PD, Maunsell FC, et al. Factors associated with colostral specific gravity in dairy cows. J Dairy Sci 2001; 84: 937-943.
15. Bradley JA. Serum Immunoglobulin levels in suckled beef calves: Quantity or quality? Can Vet J 1985; 26(3): 118-119.
16. Pauli JV. Colostral transfer of gamma glutamyl transferase in lambs. New Zealand Veterinary Journal 1983; 31(9): 150-151.
17. Stafford KJ, Mellor DJ, Todd SE et al. The physical state and plasma biochemical profile of young calves on arrival at a slaughter plant. NZ Vet J, 2001; 49: 142-149.
18. Braun JP, Tainturier D, Laugier C, et al. Early variations of blood plasma gamma-glutamyl transferase in newborn calves - a test of colostrum intake. J Dairy Sci. 1982; 65: 2178-2181.
19. Bilal T. Yeni Doğanların İç Hastalıkları, İstanbul Üniversitesi Yayın No: 4657, Veteriner Fakültesi Yayın No:25, İstanbul: 2007: 132-433.
20. Perino LJ, Sutherland RL, Woolllen NE. Serum γ- glutamyltransferase activity and protein concentration at birth and after suckling in calves with adequate and inadequate passive transfer of immunoglobulin G. Am J Vet Res 1993; 54: 56-59.
21. Wilson LK, Tyler JW, Besser TE, et al. Prediction of serum G1 concentration in beef calves based on age and serum gamma glutamyl transferase activity. J Vet Intern Med 1999;13: 123-125.