İnkübasyon ve inkübasyondan sonraki bazı dönemlerde bıldırcın(Coturnix coturnix japonica) üropigi bezinin histokimyasal yapısı

Bu çalışma inkübasyonun 13., 15. ve 17. günleri ile inkübasyon sonrası 1 haftalık dönemde bıldırcın (Coturnix coturnix japonica) üropigi bezinin histokimyasal özelliklerinin belirlenmesi amacıyla yapıldı. İnkübasyonun 13. ve 15. günlerinde uygulanan Sudan Black B ve Best’s Carmin yöntemlerine karşı üropigi bezinin hiçbir bölgesinde reaksiyon gözlenmedi. Uygulanan Nile Blue Sulphate yöntemiyle inkübasyonun 13. gününde bezin tamamında hidrofobik lipidlerin baskın olduğu belirlendi. İnkübasyonun 17. gününde ise 15. güne göre merkezi boşluğu çevreleyen epitelde ve tomurcukların uçlarındaki hücrelerde fosfolipidlerin daha zayıf reaksiyon gösterdiği saptandı. 1 haftalık dönemde üropigi bezinin glikojen bölgesine bakan kısımlarında esterler ve trigliseridlerin, glikojen içeren bölgesinde bulunan hücrelerde ve tubuller arası bağ dokuda ise glikojenin varlığı saptandı. Üropigi bezinin merkezi lumeni çevreleyen hücrelerde fosfolipidlerin ve yağ bölgesindeki tubuluslarda serbest yağ asitlerinin bulunduğu belirlendi.

The histochemical structure of uropygial gland of the quail (Coturnix coturnix japonica) in some periods of incubation and after incubation

Present study was aimed to determine the histochemical properties of uropygial gland of quail (Coturnix coturnix japonica) during 13th, 15th and 17th days of incubation and periods a week after incubation. Nothing area of uropygial gland was showed positive reaction opposite to Sudan Black B and Best’s Carmin methods in 13th and 15th days of incubation. Hidrophobic lidips were found dominant in Nile Blue Sulphate method to 13th day of incubation. Fosfolipid showed weak reaction in epithelia and edge cells of tubules surrounded around of central lumen in17th day of incubation than 15th day of incubation. It was detected that esters and trigliserids in layers near in the glycogen region detected in glycogen intertubuler connective tissue and in cells existed in the glycogen region of uropygial glands during a week period. The existance of free fatty acids in tubules in the sebaceous region and phospholipids cells in the lining central cavity of uropygial glands were determined.

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