Elektromanyetik radyasyona maruz bırakılan kobayların böbrek dokusunda prooksidan-antioksidan düzeylerine yeşil çayın etkisi

Bu çalışmada cep telefonlarından kaynaklanan elektromanyetik radyasyonun kobay böbrek dokusunda meydana getirebileceği oksidatif hasarın tespit edilmesi, yeşil çay ekstraktının bu hasarı önleyici veya koruyucu etkisinin olup olmadığının ortaya çıkarılması amaçlanmıştır. Böbrek dokusunda malondialdehit (MDA), nitrat, nitrit ve glutatyon (GSH) düzeyleri ve süperoksit dismutaz (SOD) ve glutatyon peroksidaz (GSH-Px) enzim aktiviteleri ölçüldü. Çalışma için 600-800 gr ağırlığında 28 adet kobay seçilerek, her grupta 7 kobay olacak şekilde 4 gruba ayrıldı. Grup A (n= 7) : Kontrol grubu normal diyetle beslendi ve standart yetiştirme uygulandı. Grup B (n= 7) : Kobaylar 30 gün boyunca, günde 20 dakika konuşma, 23 saat 40 dakika da bekleme modunda çalışan cep telefonlarının yaydığı 900-MHz elektromanyetik alana maruz bırakıldı. Grup C (n= 7) : Bir ay süreyle hem elektromanyetik alana maruz bırakıldı ve hem de 100 mg/kg günlük doz olarak ağızdan yeşil çay ekstraktı uygulandı. Grup D (n=7) : Bir ay süresince 100 mg/kg günlük doz olarak ağızdan yeşil çay ekstraktı uygulandı. Sadece elektromanyetik radyasyon uygulanan B grubunun MDA, nitrat ve nitrit düzeylerinde kontrol grubuna göre istatistiki açıdan anlamlı artışlar (p≤0,001), GSH, SOD ve GSH-Px aktivitelerinde ise önemli azalmalar (p≤0,001) tespit edildi. Elektromanyetik radyasyona maruz kalınmasının kobay böbrek dokusunda oksidatif strese neden olduğu ve önemli bir antioksidan olan yeşil çay ekstraktı kullanımının böbrek dokusunda meydana gelen oksidatif stresi azaltıcı yönde etki gösterdiği gözlemlendi.

Effects of green tea on prooxidant-antioxidant levels of kidney tissue in guinea pigs exposed to the electromagnetic radiation

In this study, it was aimed to determine the probable oxidative destruction of electromagnetic radiation sourcing of cellular phones on the kidney tissue of guinea pigs and reveal whether green tea extract has a preventive and protective effect to this damage. Malondialdehyde (MDA), nitrate, nitrite, and glutathione (GSH) levels and superoxide dismutase (SOD), and glutathione peroxidase (GSHPx) enzyme activities were measured in the kidney tissues. For the study, 28 guinea pigs weighing 600–800 gr were chosen and divided to 4 groups each has 7 guinea pigs. Group A as the control group, it was fed with standart diet and kept under normal breeding conditions. Group B animals were exposed to 20 minutes talking for 30 days and were exposed to 23 hours and 40 minutes 900 MHz electromagnetic radiation spreading from cellular phone working of standby mode. Group C was both exposed to electromagnetic radiation and 100 mg/kg green tea extract was given orally per day for a month. Group D animals were treated orally green tea extract of 100 mg/kg/bw every day for a month. Comparing to control group, it was determined that only the levels of MDA, nitrate and nitrite were statistically increased (p≤0,001) and the levels of GSH, SOD, and GSH-Px were significantly decreased (p≤0,001) in Group-B exposed to electromagnetic radiation. It was observed that the exposure of guinea pigs to electromagnetic radiation causes oxidative stress on kidney tissues and, green tea extract as an important antioxidant has a decreasing effect to this oxidative stress in kidney tissues.

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