Kayseri ilinde satılan çiğ sütlerde Staphylococcus aureus ve Enterotoksinlerinin varlığı üzerine araştırmalar

Bu çalışmada, Kayseri ilinde satışa sunulan çiğ sütlerde bulunan Staphylococcal Enterotoksinlerin (SEs) ELISA testleri ile tespiti ve çiğ sütlerden izole edilen S.aureus izolatlarının sahip olduğu enterotoksin genlerinin m-PCR metodu ile belirlenmesi amaçlandı. Çalışmada, incelenen 100 adet çiğ süt numunesinin 60’ında (%60) Staphylococcus spp. üremesi tespit edildi. İncelenen 300 adet izolata yapılan katalaz ve koagülaz testi ile 42’sinin (%14) S. aureus olduğu belirlendi. Belirlenen 42 adet S. aureus izolatının m-PCR metodu ile incelenmesi sonucunda 31 izolatta (%73.8) enterotoksin genlerininin varlığı tespit edildi. Bu enterotoksinlerin sırasıyla 12’sinin (%38.7) sea, 2’sinin (%6.5) seb, 5’inin (%16.1) sec, 10’ unun (%32.3) sed ve 2’sinin (%6.5) hem sea hem de sed gen dağılımına sahip olduğu belirlendi. Belirlenen 42 adet izolata ait çiğ sütlerin ELISA tekniği ile incelenmesi sonucunda ise 28 örnekte (%66.6) SEs varlığı belirlendi. Uygulanan ELISA testleri sonucunda numunelerdeki SEs’lerin sırasıyla 10’unun (%35.7) SEA, 2’sinin (%7.1) SEB, 5’inin (%17.8) SEC, 9’unun (%32.2) SED ve 2’sinin (%7.1) hem SEA hem de SED olduğu tespit edildi. S. aureus izole edilmeyen çiğ sütlerde SEs varlığına rastlanmadı. Bu çalışmada, Kayseri ili ve merkez ilçelerde satışa sunulan çiğ sütlerde S. aureus ve enterotoksinlerinin varlığı ortaya konulmuş ve bu toksinlerin halk sağlığı açısından göz ardı edilmemesi gereken bir tehlike oluşturabileceği sonucuna varılmıştır.

Investigation of the presence of S. aureus and Staphylococcal enterotoxins in raw milk samples retailed in Kayseri

The aim of this study was to identify Staphylococcal enterotoxins (SEs) by ELISA in raw milk samples marketed in Kayseri, and to detect S. aures isolates having enterotoxin genes using mPCR method in raw milk samples. Sixty out of 100 raw milk samples (60%) were identified with Staphylococcus spp. 42 out of 300 isolates (14%) studied were identified as S. aures using catalase and coagulase tests. The presence of Staphylococcal enterotoxin genes was identified in 31 out of 42 S. aureus isolates (73.8%) by using mPCR method. It was determined that these enterotoxins contained 12 (38.7%) sea, 2 (6.5%) seb, 5 (16.1%) sec, 10 (32.3 %) sed ve 2 (6.5%) both sea and sed gene distribution, respectively. The presence of SEs was identified in 28 out of 42 raw milk samples (66.6%) by using ELISA technique. It was determined that these SEs had 10 (35.7%) SEA, 2 (7.1 %) SEB, 5 (17.8 %) SEC, 9 (32.2%) SED ve 2 (7.1%) both SEA and SED distribution, respectively. No SEs was identified in raw milk samples without S. aureus. In this study, the presence of S. aureus and their enterotoxins were effective in raw milk retailed in Kayseri and vicinity and it was concluded that these toxins should not be ignored for public health.

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