Ratlarda diyabet öncesi ve sonrası oksidan-antioksidan durum

Bu çalışmada, sağlıklı ve diyabetik ratlarda serbest radikaller ve antioksidan ilişkisinin araştırılması amaçlandı. Bunun için sağlıklı ve diyabet oluşturulmuş ratlarda Homosistein, Leptin, A, E, C vitamin düzeylerinin ve bazı lipid bileşenlerinin seviyeleri ile MDA belirlendi. Bu amaçla 10 haftalık, 20 adet sağlıklı ve 20 adet deneysel olarak diyabet oluşturulmuş ratda HCY, leptin, Vit A, E, C ve bazı lipid bileşenleriyle MDA çalışıldı. Diyabetik grupta, kontrol grubuna göre plazma MDA seviyesi artarken (p

Oksidan and antioksidant situation before and after diabets in rat

In this study, we aimed to investigate correlation between free radicals and antioxidants in healthy and diabetic rats. For this purpose, we assessed Homocystein, Leptin, Vitamin A, E, C levels and some lipid parameters levels with MDA in healthy and diabetes-induced rats. In this context, HCY, leptin, Vit A, E, C and some lipid parameters with MDA were evaluated in 10 weeks old 20 healthy and 20 rats with experimentally-induced diabetes. Plasma MDA levels were found to be significantly increase in diabetic group when compared to control group (p

___

  • 1. Vincent AM, Russell JW, Low P, Feldman EL. Oxidative Stress in the Pathogenesis of Diabetic Neuropathy. Endocrine Reviews 2004; 25: 612-628.
  • 2. Memisogullan R, Taysi S, Bakan E, Capoglu I. Antioxidant Status and Lipid Peroxidation in Type II Diabetes Melhtus. Cell Biochem Func 2003; 21: 291-296.
  • 3. Sacks DB. Diabetes mellitus. In: Burtis CA, Ashwood ER, (Editors). Tietz Texbook of Clinical Chemistry. Philadelphia: WB Saunders Co 1999; 766-776.
  • 4. Cherubini A, Ruggiero C, Polidori MC, Mecocci C. Potential markers of oxidative stress in stroke. Free Radical Biology & Medicine 2005; 39: 841-852.
  • 5. Memisogullari R, Bakan E. Levels of ceruloplasmin, transferrin, and lipid peroxidation in the serum of patients with Type 2 diabetes mellitus. Journal of Diabetes and Its Complications 2004; 18: 193-197.
  • 6. Carlson LA, Rosenhamer G. Reduction of mortality in the stokholm iskemia heart disease secondry prevention study by combined treatment with clofibrate and nicotinic acid. Acta Med Scand 1988; 223: 405-418.
  • 7. Sansoy S. Risk Faktörleri ve hiperlipidemi tedavisinde klavuz kurallar, Türkiye Klinikleri Journal of Cardiology Hiperlipidemi Özel Sayısı 2000; 13: 33-40.
  • 8. Challem J, Dolby V. Homocysteine: The Secret killer, the real cause of heard disease and stroke, Good Healty Pub 1997; 13-21.
  • 9. Jacobs NJ. Microbial oxidation of protoporhydrinogen, an intermediate in heme and chlorphyll biosynthesis. Van Denmark P.J. Arch Biochem Biophys 1980; 88: 250-255.
  • 10. Koditschek LK, Umbreit WW. Alpha-glycerophosphate oxidase in streptococcus faecium F 24: J Bacteriol 1969; 98: 1063-1068.
  • 11. Trinder P. Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen. Ann Clin Biochem 1969; 6: 24-27.
  • 12. Allain CC, Poon LS, Chan CSG, Richmond W. Enzymatic determination of total serum cholasterol. Clin Chem, 1974; 20: 470-475.
  • 13. Ehret W, Heil W, Schmitt Y, Topfer G, et al. Use of anticoagulants in diagnostic laboratory investigations and stability of blood, plasma and serum samples; WHO/DIL/LAB/ 1999; Rev.2/26pp.
  • 14. Mikl YA. Homoneoues assay for the selective measument of LDL cholesterol in serum. Enzymatic selective protection method. Clin Lab 1999; 45: 398-401.
  • 15. Sushil KJ, Mc Coy B, Wise R. Vitamin E and hpercoagulability of neonatal blood. Clin Chem Acta 1994; 225; 97-103.
  • 16. Comstock GW, Alberg AJ., Helzlouer KJ. Reported effects of long-term freezer storage on concentrations of retinol, β- carotene, and α- tocopherol in serum or plasma summarized. Clin Chem 1993; 39(6): 1075-1078.
  • 17. Sundrehagen E. Axis Biochemicals ASA. Enzymatic essay for homocysteine and a kit therefor. EP 623174/US5631127.
  • 18. Considine RV, Sinha MK, Heinman ML, et al. Serum immunoreactive leptin concentrations in normal weight and obese hummans. New England J Med 1996; 334: 292.
  • 19. Placer ZA, Cushmann LL, Johnson BC. Estimation of products of lipid peroxidation in biochemical systems. Anal Biochem 1966; 16, 359-364.
  • 20. Köseoğlu MH, Fadıloğlu M, Çelik Y, Güneri S. Koroner kalp hastalığında serum malondialdehid düzeylerinde oluşan değişiklikler, Dokuz Eylül Üniversitesi Tıp Fakültesi Dergisi 1999; 77-88.
  • 21. Nedrebo BG, Ericsson UB, Nygard O. Plasma total homocysteine levels in hyperthyroid and hyperthyroid patients. Metabolism 1998; 47: 89-93.
  • 22. Demirbas B, Özkaya M, Cakal E. Plasma homocystein levels in hyperthyroid patients. Endocr J 2004; 51: 121- 125.
  • 23. Hofman MA. Hyperhomocysteinemia and endothelial dysfunction in IDDM. Diabetes Care 1997; 20; 1880- 1886.
  • 24. Chico A, Perez A, Cordoba A, Arcelus R, Carreras G. Plasma homocysteine is related to albumin excretion rate in patients with diabetes mellitus, Diabetologia 1998; 41: 684-693.
  • 25. Boushey CJ, Beresford SA, Omenn GS, Motulsky AG. A quantitative assessment of plasma homocysteine as a risk factor for vascular disease. Probable benefits of increasing folic acid intakes. Jama 1995; 274: 1049-1057.
  • 26. Robillon JF, Canivet B, Candito M, et al. Type 1 diabetes mellitus and homocysteine. Diabetes Metab 1994; 20: 494-496.
  • 27. Wing RR, Koeske R, Epstein LH, et al. Long-term effects of modest weight loss in type 2 diabetic patients. Arch Intern Med 1987; 147: 1749-1753.
  • 28. Schwartz MW, Seeley RJ. Neuroendokrine responses to starvation and weight loss. N Engl J Med 1997; 336: 1802- 1811.
  • 29. Harwey J, Mc Kenna F, Herson PS, Spanswick D, Ashford LJ. Leptin activates ATP-sensitive potassium channels in rat insulin-secreting cell line, CRI-G1. J Physiol 1997; 504: 527-535.
  • 30. Serafinowicz A, Kukula TC, Shaibani T, Baczkowska JS, Sadowska A. Homocysteine and lipid peroxidation products: important atheriosclerosis risk factors in renal allograft recipients. Transplantation Proceedings 2000; 32: 1367-1368.
  • 31. Itateyama E, Chiba S, Skata T, Yoshimatsu H. Hypothalamic neuronal histamine in genetically obese animals: its implication of leptin action in the brain. Exp Biol (Maywood) 2003; 228 (10): 1132-1137.
  • 32. Mantzoros CS, Moschos S, Avramopoulos I, et al. Leptin concentrations in relation to body mass index and the tumor necrosis factor-system in humans. J Clin Endocrinol Metab 1997; 82: 3408-3413.
  • 33. McGregor GP, Desaga JF, Ehlenz K, et al. Radioimmunological measurement of leptin in plasma of obese and diabetic human subjects. Endocrinology 1996; 137: 1501-1504.
  • 34. Sinclair AJ. Free radical mechanisms and vascular complication of diabetes mellitus. Diabetes Rev 1939; 1 (2): 7-10
  • 35. Çömlekçi A, Biberoğlu S, Kozan O, et al. Correlation between serum lipoprotein(a) and angiographic coronary artery disease in non-insulin-dependent diabetes mellitus. J Intern Med 1997; 242: 449-454.
  • 36. Godin DV, Wohaieb SA, Garnett ME. Goumeniouk, AD. Antioxidant enzyme alterations in experimental and clinical diabetes, Molecular and Cellular Biochemistry 1998; 84: 223-231.
  • 37. Anderson R, Theron AJ, Ras GJ. Cysteine and dapsone of the increased extrasellular and intracellular generation of reactive oxidants by activated phagocytes from cigarette smokers. Am Rev Respir Dis 1987; 135(5): 1027-1032.
  • 38. Foody JM, Milberg JA., Pearce GL., Sprecher DL. Lipoprotein (a) associated with coronery artery disease in older women: age and gender analysis. Atherosclerosis 1999; 153: 445-451.
  • 39. Griesmacher A, Kindhauser M, Andert SE, Schreiner W., et al. Enhanced Serum levels of thiobarbituric acid - reactive substances in diabetes mellitus. Am J Med 1995; 98: 469-475.
  • 40. Giugliano D, Ceriello A. Oxidative stress and diabetic vascular complications. Diabetes care 1996; 19: 257-267.
  • 41. Nerup J, Mandrup-Poulsen T, Helwuist S, et al. On the pathonenesis of IDDM. Diabetologia 1994; 37: 82-89.
  • 42. McLennan SV, Heffernan S, Wright L, Rae C, et al. Changes in hepatic glutathione metabolism in diabetes, Diabetes 1991; 40, 344-348.
  • 43. Asayama K, Nakane T, Uchida N, Dobashi K, Nakazawa S. Serum antioxidant status in streptozotocin-induced diabetic rat. Horm Metab Res 1994; 26: 313-315.
  • 44. Diplock AT. Antioxidant nutrients and disease prevention: An overview. Am J Clin Nutr 1991; 53:1895-1935.
  • 45. Celik S, Akkaya H. Total Antioxidant Capacity, Catalase and Superoxide Dismutase on Rats Before and After Diabetes. Journal of Animal and Veterinary Advences 2009; 8(8): 1503-1508.
Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi-Cover
  • ISSN: 1308-9323
  • Yayın Aralığı: Yılda 3 Sayı
  • Yayıncı: Prof.Dr. Mesut AKSAKAL
Sayıdaki Diğer Makaleler

Latent asidotik stres

Yusuf GÜL

Koyun dalak doku arjinazının fotoinaktivasyonu ve bazı kinetik özellikleri

Necmi ÖZDEMİR, Fatih Mehmet KANDEMİR, Mehtap ÖZÇELİK

İnkübasyon ve inkübasyon sonrası dönemin bazı evrelerinde bıldırcınlarda (Coturnix coturnix japonica) dil bezlerinin histolojik gelişimi ve histokimyasal yapısı

Kenan ÇINAR, Nagehan ÇİMENOĞLU

Kayseri ilinde satılan çiğ sütlerde Staphylococcus aureus ve Enterotoksinlerinin varlığı üzerine araştırmalar

Nurhan ERTAŞ, Zafer GÖNÜLALAN

Yeni doğan buzağılarda çeşitli Hematolojik ve Biyokimyasal parametreler İle kolostral immun globulinler arasındaki ilişkinin belirlenmesi

Didem PEKMEZCİ, Yücel MERAL, Güvenç GÖKALP, E. Emek ONUK, Duygu ÇAKIROĞLU

Ratlarda diyabet öncesi ve sonrası oksidan-antioksidan durum

Sait ÇELİK, Hatice AKKAYA

Bir Atta akut levamizol zehirlenmesi

Onur BAŞBUĞ, Mustafa İSSİ, Yusuf GÜL

İnkübasyon ve inkübasyondan sonraki bazı dönemlerde bıldırcın(Coturnix coturnix japonica) üropigi bezinin histokimyasal yapısı

Kenan ÇINAR, Seval KELEK

Koyun böbrek doku Arginazının etilen diamin tetra asetik asit (EDTA), para kloromerküri benzoik asit (P-CMBA), N- Etil maleimid (NEM) tarafından inhibisyonu ve inhibisyon kinetiği

Necmi ÖZDEMİR, Fatih Mehmet KANDEMİR, Mehtap ÖZÇELİK

Elektromanyetik radyasyona maruz bırakılan kobayların böbrek dokusunda prooksidan-antioksidan düzeylerine yeşil çayın etkisi

Mustafa CELLAT, Dide Kılınç KILIÇALP