2011

The efficiency of cauterization of clitoris on serum lh levels in cows

Sunulan çalışma, ineklerde klitoris koterizasyonunun Luteinleştirici Hormon (LH) salınımı üzerine etkisinin araştırılması amacıyla yapıldı. Çalışmanın materyalini, yaşları 2,5–6 arasında değişen toplam 10 inek oluşturdu. Materyal olarak kullanılan hayvanların östrüsleri 11 gün arayla çift doz PGF2α enjeksiyonu ile senkronize edildi. Senkronizasyon sonrası şekillenen ilk tabii siklus kontrol siklusunu oluşturdu. İlk östrüs belirtisi görüldüğü andan itibaren 24 saat boyunca, 2 saat aralıklarla, LH ölçümü amacıyla kan numuneleri alındı. Kendiliğinden şekillenen ikinci siklusta ilk östrüs belirtisinin görülmesiyle birlikte, hayvanın klitorisi epidural anestezi altında koterize edilerek, kontrol siklusunda olduğu gibi, kan numunelerinin toplanmasına devam edildi. Kontrol ve uygulama grubunda ortalama LH değerlerinin sırasıyla 2,18±0,30 ng/ml ve 2,03±0,31 ng/ml olduğu belirlendi. Ortalama LH pik değeri kontrol grubunda 11.56±1.99 ng/ml, uygulama grubunda ise 10.55±1.63 ng/ml; östrus başlangıcı-LH pik zamanı arasında geçen süre kontrol grubunda 16±2.61 saat, uygulama grubunda 12±0.97 saat olarak belirlendi. Pik süresi kontrol grubunda 8.4±0.75 saat, uygulama grubunda 10.4±0.4 saat olarak tespit edildi. Yapılan uygulamanın kontrol ve uygulama grubunda LH parametrelerini etkilemediği görüldü. Sonuç olarak, ineklerde östrüs başlangıcında uygulanan klitoris koterizasyonunun, ortalama LH değerlerini etkilemediği tespit edildi.

İneklerde klitoris koterizasyonunun lh salınımı üzerine etkisinin araştırılması

The aim of the present study was to investigate the effect of cauterization of clitoris on serum Luteinizing Hormone (LH) levels in cows. In this study, 10 cows ranging from 2,5–6 years old were used. Animals were synchronised with double injections of PGF2α at 11 day intervals. After the first sign of spontaneous estrous, blood samples were collected at 2 hours intervals during 24 hours to monitor LH concentration. When the second spontaneous estrous occurred, clitoris of cows was cauterized with electrocoter under epidural anesthesia and blood samples were collected same as the first spontaneous cycle. LH concentrations were found 2.18±0.30 ng/ml, 2.03±0.31 ng/ml in control and clitoral cauterization group, respectively. Preovulatory LH peak concentrations were measured 11.56±1.99 ng/ml in control group and 10.55±1.63 ng/ml in cauterization group. The time from the beginning of estrous to the LH peak was found 16±2.61 h in control group and 12±0.97 h in cauterization group. Average duration of LH surge was observed 8.4±0.75 h and 10.4±0.4 h in control and cauterization group, respectively. No significant difference was observed between control and experimental group. As a conclusion, it was detected that clitoral cauterization applied at the beginning of estrous in cows did not affect serum LH values.

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1.Frandson RD, Wılke WL, Fails AD. Anatomy of the female reproductive system: In: Frandson, RD. (Editor) Anatomy and Physiology of Farm Animals. 7th Edition, USA: Wiley- Blackwell, 1975: 427.
2.Hafez ESE, Jainudeen MR, Rosnina Y. Physiology of reproduction. In: Hafez, B, Hafez, ESE. (Editor) Reproduction in Farm Animals. 7th Edition, Lippincott Baltimore, USA: Williams & Wilkins, 2000: 28-54.
3.Custer EE, Berardinelli JG, Short RE, Wehrman M, Adair R. Postpartum interval to estrus and patterns of LH and progesterone in first-calf suckled beef cows exposed to mature bulls. J Anim Sci 1990; 68: 1370-1377.
4. Lunstra DD, Laster DB. Influence of single-sire and multiple-sire natural mating on pregnancy rate of beef heifers. Theriogenology 1982; 18: 373-382.
5. Nikolakopoulos E, Kindahl H, Gilbert CL, Goode J, Watson ED. Release of oxytocin and prostaglandin F2α around teasing, natural service and associated events in the mare. Anim Reprod Sci 2000; 63: 89-99.
6.Karaca F, Gülyüz F, Alan M, Taşal I. İneklerde sun’i tohumlama sonrası klitorise masaj ve kas içi oksitosin uygulamalarının gebelik oranına etkisi. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi 2001; 12: 50-52.
7.Stupnicki R. Recent developments in animal reproduction physiology and endocrinology in Poland. J Anim Sci 1975; 40: 1307-1315.
8.Lunstra DD, Hays WG, Bellows RA, Laster DB. Clitoral stimulation and the effect of age, breed, technician and postpartum interval on pregnancy rate to artificial insemination in beef cattle. Theriogenology 1983; 19: 555- 563.
9.Singh M, Vasishta NK, Sood P, Kapur V. Effect of clitoral stimulation after artificial insemination on conception in cattle. Indian Vet J 2001; 78: 947-948.
10.Randel RD, Short RE, Christensen DS, Bellow RA. Effect of clitoral massage after artificial insemination on conception in the bovine. J Anim Sci 1975; 40: 1119-1123.
11. Randel RD, Short RE, Christensen DS, Bellow RA. Effects of various mating stimuli on the LH surge and ovulation time following synchronization of estrus in the bovine. J Anim Sci, 1973; 37: 128-130.
12.Short RE, Carr JB, Graves NW, Mimline WL, Bellows RA. Effect of clitoral stimulation and length of time to complete AI on pregnancy rates in beef cattle. J Anim Sci 1979; 49: 647-650.
13. Segura CVM, Rodriguez ROL. Effect of clitoral stimulation after artificial insemination on conception in zebu- crossbred heifers in the tropics. Theriogenology 1994; 42: 781-787.
14. Cooper MD, Foote RH. Effect of oxytocin, prostaglandin F2α and reproductive tract manuplations on uterine contractility in holstein cows on days 0 and 7 of the estrous cycle. J Anim Sci 1986; 63: 151-161.
15. Cooper MD, Newman SK, Schermerhorn EC, Foote RH. Uterine contractions and fertility following clitoral massage of dairy cattle in estrus. J Dairy Sci 1985; 68: 703-708.
16. Pointer J. Clitoral massage as a supproting measure in manuplation of the bovine uterus. Tierarz Prax 1986; 14: 217-218.
17. Rodriguez T, Verde O, Espinoza J. Effect of time of insemination, clitoral massage, season, breed and other factors on fertility in cattle. 9th International Congress on Animal Reproduction and Artificial Insemination, Madrid, Spain. 1980; 205.
18. Zalesky DD, Day ML, Imakawa K, Kittok RJ, Kinder JE. Effects of copulation on timing of the LH surge following snchronization of estrus in the bovine. Theriogenology 1985; 23: 663-670.
19. Arbeiter K, Pohl W, Rumpf R. Special methods of treatment to induce ovulation in cattle. Tierarztl Umschau 1985; 40: 442-450.
20. Kirsch JD, Tilton JE, Ziecik A, Weighl R, Schaffer T, Williams GL. Effects of various mating stimuli on pituitary release of luteinizing hormone in the gilt. Domest Anim Endocrinol 1985; 2: 99-104.
21. Mutayoba BM, Meyer HDD, Schams D, Schallenberger E. Development of a sensitive enzyme immunoassays for LH determination in bovine plasma using the streptavidin- biotine tecnique. Acta Endocrin 1990; 122: 227-232.
22. Robertson HA, Rakha AM. The timing of the neural stimulus which leads to ovulation in the sheep. J Endocrinol 1965; 32: 383-386.
23. Tilton JE, Ziecik A, Kirsch J, Weigl R. Patterns of LH release in pigs during estrus. J Anim Sci 1980; 51: 89.
24.Randel RD, Garverıck HA, Surve AH, Erb RE, Callahan CJ. Reproductive steroids in the bovine. V. Comparisons of fertile and nonfertile cows 0 to 42 days after breeding. J Anim Sci 1971; 33: 104-114.
25. Walters DL, Schallenberger E. Pulsatile secretion of gonadotropins, ovarian steroids and ovarian oxytocin during the periovulatory phase of the estrous cycle in the cow. J Reprod Fertil 1984; 71: 503-512.
26. Garverick HA, Erb RE, Niswender GD, Callahan CJ. Reproductive steroids in the bovine. III. changes during the estrous cycle. J Anim Sci 1971; 32: 946-956.
27. Panth HC, Barot LR, Kasiraj L, Mısra AK, Prabhakar JH. Effect of clitoral stimulation after artificial insemination on conception rate in the buffalo. Bubalus Bubalis 2001; 73: 66-69.
28. Ziecik A, Tilton JE, Williams GL. Effect of mating on the luteinizing hormone surge in the pig. J Anim Sci 1981; 53: 434-438.