Fatih Mehmet KANDEMİR, Murat YÜKSEL, Mine ERİŞİR

İneklerin sütündeki mda, gsh düzeyleri ile gsh-px, cat aktiviteleri üzerine subklinik mastitisin etkisi

Subklinik mastitis sık karşılaşılan mastitis formu olup süt üretiminde azalma ve süt kalitesinde düşme ile karakterizedir. Bu çalışmada subklinik mastitisin ineklerin sütündeki oksidan (malondialdehit, MDA) ve antioksidan (glutatyon, GSH; glutatyon peroksidaz, GSH-Px ve katalaz, CAT) durum üzerine etkisinin araştırılması amaçlandı. Araştırmanın materyalini, yaşları 4-8 arasında değişen, farklı ırklardan, toplam 40 baş inek oluşturdu. İneklerde mastitis teşhisi California Mastitis Testi (CMT) ve +1+2+3 bakteriyolojik muayene ile yapıldı. Kontrol, CMT , CMT , CMT olmak üzere dört grup oluşturuldu. Mastitis teşhisi konulan meme loblarından süt numunesi alınarak MDA ve GSH düzeyleri ile GSH-Px ve CAT aktiviteleri ölçüldü. MDA düzeyleri ile GSH-Px ve CAT aktivitelerinde istatistikî açıdan önemli fark bulunmadı (P>0.05). Bununla birlikte, mastitisin derecesine bağlı olarak GSH-Px aktivitesinde istatistik olarak önemli olmayan azalma tespit edildi. Süt GSH düzeyinde mastitisin derecesine bağlı +3 olarak saptanan azalma CMT şiddetindeki mastitisde istatistik olarak önemli bulundu (P

The effect of subclinical mastitis on mda, gsh levels and gsh-px, cat activities in milk of cows

Subclinical mastitis is one of the most observed mastitis forms in dairy cattle resulting in decreased milk production and quality. The aim of this study was to investigate the oxidant (malondialdehyde, MDA) and antioksidant (glutathione, GSH; glutathione peroxidase, GSH-Px; catalase, CAT) status in milk of cows with subclinical mastitis. A total of 40 cows, 4-8 ages and various breeds, were used as experimental material. Subclinical mastitis was diagnosed by California Mastitis Test (CMT) and bacteriological examination of milk samples. Four groups consisted of control, CMT+1 , CMT+2 , CMT +3 were designed. The milk samples from mammary lobes with mastitis were collected. MDA, GSH levels and GSH-Px, CAT activities in the milk were determined. No statistically significant difference was found MDA level and GSH-Px, CAT activities (P>0.05). However, with increasing mastitis degree, GSH-Px activity was decreased but not significantly. Decreasing in milk GSH levels with increasing mastitis degree was found statistically significant for CMT+3mastitis (P

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