Fatih Mehmet KANDEMİR, Ali Said DURMUŞ, Mehmet Cengiz HAN, Aydın SAĞLIYAN, Mustafa ÖZKARACA

Propolis ve Hypericum perforatum Krem’in Yanık Yara İyileşmesi Üzerine Etkileri

Bu çalışmada, Propolis (Pr) ve Hypericum perforatum (HP) kreminin, bir rat modelinde yanık yaralarında bir dizi biyokimyasal ve histopatolojik parametreler üzerindeki etkilerini değerlendirmeyi amaçladık. Kırk iki dişi 3 aylık Wistar Albino rat üç eşit ana gruba ayrıldı. Bu ana grupların her biri, 7 ve 14 günlük postoperatif takipler için iki alt gruba ayrıldı. Genel anestezi altında tüm ratların sırt derisi üzerinde iki adet 10 mm çapında ikinci derece termal yanık oluşturuldu. Birinci, ikinci ve üçüncü gruplardaki yanmış deri bölgeleri sırasıyla plasebo krem (kontrol), %50 Pr krem ve % 50 HP krem ile kaplandı (her grup için n= 14). Postoperatif 7 ve 14 günlük takip sürelerini tamamlayan ratlar ötenazi edildi ve deri örnekleri biyokimyasal ve histolojik olarak incelendi. Sonuçlar Pr'nin diğer gruplara göre daha fazla antioksidan özelliği olduğunu gösterdi. Pr ve HP, epitelizasyon, granülasyon dokusu ve kollajen için teşvik edici özelliği ile yanık yara iyileşme etkisini arttırdı (P

Effects of Propolis and Hypericum perforatum Cream on Burn Wound Healing

This study aimed to evaluate the effects of Propolis (Pr) and Hypericum perforatum (HP) cream ona number of biochemical and histopathological parameters in burn wounds in a rat model. Fortytwo female 3-month-old Wistar Albino rats were assorted into three equal main groups. Each ofmain groups was assorted into two subgroups for postoperative 7 and 14 days follow-ups.Bilaterally 10 mm in diameter second degree thermal burn wounds were created on the back skinof all rats under general anaesthesia. The burned skin areas in the first, second and third groupswere coated with placebo cream (control), 50% Pr cream, and 50% HP cream twice a day,respectively (n= 14 for each group). The rats that completed postoperative follow-up periods of 7and 14 days were euthanized and skin samples were examined biochemically and histologically.Results showed that Pr had more antioxidant property than the other groups. Pr and HP increasedthe burn wound healing effect with its encouraging property for epithelisation and granulation tissueand collagen (P

PDF

___

Durmuş AS, Yaman M, Can HN. Effects of extractum cepae, heparin, allantoin gel and silver sulfadiazine on burn wound healing: an experimental study in a rat model. Vet Med Czech 2012; 57: 287-292.
Yaman İ, Durmuş AS, Çeribaşı S, Yaman M. Effects of Nigella sativa and silver sulfadiazine on burn wound healing in rats. Vet Med Czech 2010; 55: 619-624.
Durgun T, Durmuş AS. Köpeklerde anal kese yangılarının sağaltımında propolis ekstraktının kullanımı. Doğu Anadolu Araştırmaları Dergisi 2004; 3: 159-161.
Han MC, Durmuş AS, Karabulut E, Yaman İ. Effects of Turkish propolis and silver sulfadiazine on burn wound healing in rats. Revue Med Vet 2005; 156: 624-627.
Martinotti S, Ranzato E. Propolis: A new frontier for wound healing? Burns Trauma 2015, 3: 1-7.
Di Paola R, Mazzon E, Muia C, et al. Protective effect of Hypericum perforatum in zymosan-induced multiple organ dysfunction syndrome: Relationship to its inhibitory effect on nitric oxide production and its peroxynitrite scavenging activity. Nitric Oxide 2007; 16: 118-130.
Han MC, Durmuş AS, Sağlıyan A, et al. Effects of Nigella sativa and Hypericum perforatum on wound healing. Turk J Vet Anim Sci 2017; 41: 99-105.
Linde K. St. John’s wort - an overview. Forsch Komplementärmed 2009; 16: 146-145.
Pesin Suntar I, Kupeli Akkol E, Yilmazer D, et al. Investigations on the in vivo wound healing potential of Hypericum perforatum L. J Ethnopharmacol 2010; 127: 468-477.
Sayar H, Gergerlioglu N, Seringec N, et al. Comparison of efficacy of topical phenytoin with hypericin in seconddegree burn wound healing: An experimental study in rats. Med Sci Monit Basic Res 2014; 20: 36-46.
Paterniti I, Briguglio E, Mazzon E, et al. Effects of Hypericum perforatum, in a rodent model of periodontitis. BMC Complement Altern Med 2010; 10: 73.
Isla MI, Moreno MIN, Sampietro AR, Vattuone MA. Antioxidant activity of Argentine propolis extracts. J Ethnopharmacol 2001; 76: 165-170.
Durmuş AS, Han MC, Yaman İ. Comparative evaluation of collagenase and silver sulfadiazine on burned wound healing in rats. FÜ Sağ Bil Vet Derg 2009; 23: 135-139.
Hosnuter M, Gurel A, Babuccu O, et al. The effect of CAPE on lipid peroxidation and nitric oxide levels in the plasma of rats following thermal injury. Burns 2004; 30: 121-125.
Placer ZA, Cushmanni LL, Johnson BC. Estimation of products of lipid peroxidation (as malondialdehyde) in biochemical systems. Anal Biochem 1966; 16: 359-364.
Aebi H. Catalase. In: Bergmeyer HU (Editor). Methods in Enzymatic Analysis. 1 Edition, NewYork: Academic Press, 1983: 276-286.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951; 193: 265-275.
Sedlak J, Lindsay RHC. Estimation of total protein bound and nonprotein sulfhydryl groups in tissue with Ellmann’s reagent. Anal Biochem 1968; 25: 192-205.
Matkovics B, Szabo I, Varga IS. Determination of enzyme activities in lipid peroxidation and glutathione pathways. Laboratoriumi Diagnos 1988; 15: 248-249.
Sun Y, Larry WO, Ying L. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988; 34: 497- 500.
Atiba A, Marzok M, Ghazy A. Comparison of Aloe vera and silver sulfadiazine in the treatment of deep seconddegree burn in dogs. Glob Vet 2014; 13: 733-737.
Bedi MK, Shenefelt PD. Herbal therapy in dermatology. Arch Dermatol 2002; 138: 232-242.
Farahpour MR, Habibi M. Evaluation of the wound healing activity of an ethanolic extract of Ceylon cinnamon in mice. Vet Med Czech 2012; 57: 53-57.
Gal P, Kilik R, Mokry M, et al. Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: Standardization of semi-quantitative and quantitative histological assessments. Vet Med Czech 2008; 53: 652-659.
Sabol F, Dancakova L, Gal P, et al. Immunohistological changes in skin wounds during the early periods of healing in a rat model. Vet Med Czech 2012; 57: 77-82.
Akhoondinasab MR, Khodarahmi A, Akhoondinasab M, Saberi M, Iranpour M. Assessing effect of three herbal medicines in second and third degree burns in rats and comparison with silver sulfadiazine ointment. Burns 2015; 41: 125-131.
Daryabeigi R, Heidari M, Hosseini SA, Omranifar M. Comparison of healing time of the 2 degree burn wounds with two dressing methods of fundermol herbal ointment and 1% silver sulfadiazine cream. Iran J Nurs Midwifery Res 2010; 15: 97-101.
Al-Waili N, Hozzein WN, Badr G, et al. Propolis and bee venom in diabetic wounds; a potential approach that warrants clinical investigation. Afr J Tradit Complem 2015; 12: 1-11.
Kartal M, Yildiz S, Kaya S, Kurucu S, Topcu G. Antimicrobial activity of propolis samples from two different regions of Anatolia. J Ethnopharmacol 2003; 86: 69-73.
Ocak T, Duran A, Ozyalvacli G, et al. Protective effects of montelukast and Hypericum perforatum against intestinal ischemia-reperfusion injury in hamsters. Turk J Med Sci 2014; 44: 381-386.
Guo S, DiPietro LA. Factors affecting wound healing. Journal of Dental Research 2010; 89. 219-229.
Dikmen M, Öztürk Y, Sagratini G, et al. Evaluation of the wound healing potentials of two subspecies of Hypericum perforatum on cultured NIH3T3 fibroblasts. Phytother Res 2011; 25: 208-214.
Mukherjee PK, Verpoorte R, Suresh B. Evaluation of in vivo wound healing activity of Hypericum patulum (family: Hypericaceae) leaf extract on different wound model in rats. J Ethnopharmacol 2000; 70: 315-321.
Samadi S, Khadivzadeh T, Emami A, et al. The effect of Hypericum perforatum on the wound healing and scar of cesarean. J Altern Complement Med 2010; 16: 113-117.