Mustafa Kemal SARIBAY, Cüneyt SERTKOL, Zafer CANTEKİN

Sütçü İneklerde Akut Klinik Mastitislerde Meme İçi Ozon Tedavisinin İyileştiririci Etkisi

Çalışma, ineklerde akut klinik mastitis vakalarında antibiyotik tedavisine alternatif bir yöntem olarak düşünülen ozon gazının iyileştirici etkisinin belirlenmesi amacıyla düzenlendi. Çalışmanın materyalini klinik olarak hasta olduğu tespit edilen laktasyondaki 22 baş ineğe ait 23 adet akut mastitisli meme lobu oluşturdu. Tedaviden önce bakteriyolojik ekim için süt örnekleri alındı. Birinci gruba 5 gün süreyle ve 24 saat aralıklarla 5ml/100 kg dozunda kas içi yolla antibiyotik enjekte edildi, hasta meme loblarına ise 3 gün süreyle ve 24 saat aralıklarla meme içi mastit preparatı uygulandı. İkinci gruba ise meme başı kanalından 50 ml ozon/oksijen gazı 24 saat ara ile 3 gün süreyle uygulandı. Tedavi bitiminden 7 gün sonra tüm ineklerden süt örnekleri alındı ve bakteriyolojik ekim yapıldı. Grup I’de süt örneklerinde üreyen bakterilerin tamamının (% 100) KNS olduğu görüldü. Bakteriyolojik ve klinik iyileşme oranları sırasıyla % 90.90 (10/11), % 90.90 (10/11) olarak saptandı. Grup II’de süt örneklerinde üreyen bakteriler,8 adet (% 66.66) KNS, 2 adet (% 16.66) KNS+maya ve 2 adet (% 16.66) Streptococcus spp. olarak tespit edildi. Bakteriyolojik iyileşme oranları sırasıyla % 87.50 (7/8) ve % 0 (0/2), % 0 (0/2), klinik iyileşme oranları ise sırasıyla % 87.50(7/8), % 50 (1/2) ve % 100(2/2) olarak saptandı. Sonuç olarak, meme içi ozon gazı uygulamasının KNS’dan kaynaklanan akut klinik mastitislerin tedavisinde bakteriyolojik ve klinik iyileşme üzerine oldukça etkin olduğu ve antibiyotiklere alternatif olabileceği, Streptecoccus spp. ve maya kaynaklı akut klinik mastitlerde ise iyileştirici etkisinin olmadığı kanısına varıldı.

Recovery Effect of Intramammary Ozone Therapy for Acute Clinical Mastitis in Dairy Cows

This research was carried out to determine the recovery effect of ozone gas which is thought to bean alternative method for antibiotic treatment in acute clinical mastitis cases. The material of thestudy consisted of 23 acute mastitis diagnosed mammary lobes belonging to 22 lactating cowsbreed. Aseptic milk samples were taken before the treatment. The cows in group I receivedantibiotic by intramuscular route for 5 days, also an antimastitis drug was injected to affected lobeswith 24 hours interval for 3 days. 50 mL ozone/oxygen gase was administered via teat duct with aninterval of 24 hours for 3 days to group II. Milk samples were taken and bacteriological examinationwas applied from the cows after 7 days post treatment. The entire bacteria (100%) isolated formthe samples of group I was, coagulase negative staphylococci (CNS). Bacterial and clinicalrecovery rates were 90.90% (10/11), 90.90% (10/11), respectively. The isolated bacteria in group IIwere 8 CNS (66.66%), 2 CNS+ Candida spp. (16.66%), 2 Streptococci spp. (16.66%).Bacteriologic recovery rates were obtained as 87.50% (7/8) ve 0% (0/2), 0% (0/2), respectively.Clinical recovery rates were determined as 87.50% (7/8), 50% (1/2) ve 100% (2/2), respectively. Asa result, it was concluded that, ozone gas was effective on bacteriological and clinical recovery andcan be an alternative to antibiotic treatment in acute clinical mastitis cases caused by CNS, butalso it was found that it does not have any effect on recovery of Streptococci and Candida spp.caused mastitis cases.

PDF

___

De Vliegher S, Fox LK, Piepers S, McDougall S, Barkema HW. Invited review: Mastitis in dairy heifers: nature of the disease, potential impact, prevention and control. J Dairy Sci 2012; 95: 1025-1040.
Kvapilík J, Hanus O, Barton L, et al. Mastitis of dairy cows and financial losses: an economic meta-analysis and model calculation. Bulgarian J Agric Sci 2015; 21: 1092- 1105.
Reshi AA, Husain I, Bhat SA, et al. Bovine mastitis as an evolving disease and its impact on the dairy industry. IJCRR 2015; 7: 48-54.
Owens WE, Ray JL, Yancey RJ. Comparison of success of antibiotic therapy during lactation and results of antimicrobial susceptibility tesis for bovine mastitis. J Dairy Sci 1997; 80: 313-317.
Constable P, Pyörala S, Smithj G. Guideliness for antimicrobial use in cattle. In: Guardabasi L, Jensen LB, Kruse H (Editors). Guide to Antimicrobial Use In Animals. Oxford: Blackwell Publishing Ltd, 2008: 143-160.
Bocci V. Ozone as bioregulator: pharmacology and toxicology of ozone therapy today. J Biol Reg Homeos Ag 1996; 10: 31-53.
Guzel O, Yıldar E, Erdikmen DO. Medical ozone and It’s use in veterinary surgery. J Fac Vet Med Istanbul Univ 2011; 37: 177-184.
Li I, Tighe RM, Feng F, Ledford JG, Hollingsworth JW. Genes of innate immunity and the biological response to inhaled ozone. J Biochem Mol Toxic 2013; 27: 3-16.
Ducusin RJT, Nishimura M, Sarashina T, et al. Phagocytosis of bovine blood and milk polymorphonuclear leukocytes after ozone gas administration in vitro. J Vet Med Sci 2003; 65: 535-539.
Ohtsuka H, Ogata A, Terasaki N, et al. Changes in leukocyte population after ozonated autohemoa administration in cows with inflammatory diseases. J Vet Med Sci 2006; 68: 175-78.
Duricic D, Vince S, Ablondi M, et al. Intrauterine ozone treatment of retained fetal membrane in simmental cows. Anim Reprod Sci 2012; 134: 119-124.
Ogata A, Nagahata H. Intramamary application of ozone therapy to acute clinical mastitis in dairy cows. J Vet Med Sci 2006; 2: 681-686.
Nevala M, Taponen S, Pyörala S. Bacterial etiology of bovine clinical mastitis–data from Saari Ambulatory Clinic in 2002-2003. Finnish Vet J 2004;110: 363-369.
Koivula M, Pitkala A, Pyörala S, Mantysaari E. Distribution of bacteria and seasonal and regionaleffects in a new database for mastitis pathogens in Finland. Acta Agric Scand. A Anim Sci 2007; 57: 89-96.
Schallibaum M. Mastitis-pathogens isolated in Switzerland: 1987-1996. IDF Mastitis Newsletter 2001; 24: 38.
Shpigel NY, Winkler M, Ziv G, Saran A. Clinical, bacteriological and epidemiological aspects of clinical mastitis in Israeli dairy herds. Prev Vet Med 1998; 35: 1-9.
Pillet F, Montreuil C, Roy O, et al. Field experience with a single intravenous injection of marbofloxacin 16% in lactating dairy cows with acute clinical mastitis. Revue Med Vet 2013; 164: 537-545.
Erskine RJ, Wagner S, De Graves FJ. Mastitis therapy and pharmacology. Vet Clin Food Anim Prac 2003; 19: 109-138.
Roy P, Dorisamy KA, Ramaswamy V. A note on antibiyogram of organisms causing clinical mastitis. Indian Vet J 1991; 68: 898-899.
Kalmus P, Simojoki H, Pyörala S, et al. Milk haptoglobin, milk amyloid A, and Nasetil-beta-D-glucosaminidase activity in bovines with naturally occurring clinical mastitis diagnosed with a quantitative PCR test. J Dairy Sci 2013; 96: 3662-3670.
Bastan A, Akan M, Oncel T. Investigation of efficacy of amoxycillin klavulanic acid combination in treatment of clinical mastitis in dairy cows. Vet Hek Mikrobiyol Derg 2001; 1: 51-55.
McDougall S. Efficacy of two antibiotic treatments in curing clinical and subclinical mastitis in lactating dairy cows. N Z Vet J 1998; 46: 226-232.
Pyörala S, Pyörala EO. Efficacy of parenteral administration of threeantimicrobial agents in treatment of clinical mastitis in lactating cows: 487 cases (1989-1995). J Am Vet Med Assoc 1998; 212: 407-412.
Viebahn-Hansler R, Fernandez OS, Fahmy Z. Ozone in medicine: The low-dose ozone concept–guidelines and treatment strategies. Ozone: Science&Engineering 2012; 34: 408-424.
Yanliang B, Zhenxin Z, Guojing C, et al. Application of ozone therapy in the treatment of clinical mastitis of dairy cows. China Dairy Cattle 2010; 5: 858.
Enginler SO, Sabuncu A, Kahraman BB. Comparison of intramammary ozone administration doses in dairy cows with clinical mastitis. Acta Sci Vet 2015; 43: 1260.
Leigh JA. Streptococcus uberis: A permanent barrier to the control of bovine mastitis? Vet J 1999; 157: 225-238.
Crawshaw WM, MacDonald NR, Duncan G. Outbreak of Candida rugosa mastitis in a dairy herd after intramammary antibiotic treatment. Vet Rec 2005; 156: 812-813.