Helal Kesim Uygulanan İneklerde Serum Beta-Endorfin Seviyelerinin İncelenmesi
Beta-endorfin, vücut tarafından üretilen (endojen) opioid peptidlerin önemli üyelerinden biridir. Ağrı giderici özellikleri nedeniyle, organizmanın strese bir cevabı olarak kabul edilir. Bu çalışmada, klinik açıdan sağlıklı ve gebe olmayan, aynı işletmeye ait, günlük kesim için kesimhaneye getirilen, 3-5 yaşlarında 6 montafon hibridi inek kullanıldı. Kan örnekleri, kesimden 20 dakika önce vena jugularis'ten antikoagülan içermeyen tüplere (Grup 1); kesim esnasında akan kandan (Grup 2); kesimden 3 dak. sonra baş bölgesinden akan kandan (Grup 3) alındı. Kan serumu beta-endorfin seviyeleri beta-endorfin ELISA kiti kullanılarak belirlendi. Grup 1, 2 ve 3 için serum beta-endorfin düzeyleri sırasıyla 157.0±23.1, 262.0±35.8, 574.3±95.4 pg/mL olarak belirlendi. Serum betaendorfin seviyelerinde kesim öncesi, esnası ve sonrasında anlamlı farklılıklar gözlendi. Sonuç olarak, helal kesimin hayvanlarda acıdan ziyade beta-endorfin reaksiyonuyla rahatlama sağladığı ortaya konulmuştur.
Investigation of Serum Beta-Endorphin Levels in Cows Slaughtered with Halal Slaughter Method
Beta-endorphin is one of the important members of the body-produced (endogenous) opioidpeptides. Because of its pain relieving properties, it is regarded as an answer of the organism tostress. In this study, clinically healthy and nonpregnant 6 brown swiss hybrid cows at the aged 3-5years of age, brought to the slaughterhouse which belongs to same company, were used. Bloodsamples were collected into anticoagulant free tubes from vena jugularis 20 minutes before theslaughter (Group 1); from flowing blood during bleeding (Group 2); from flowing blood, 3 minutesafter slaughter (Group 3). Blood serum beta-endorphin levels were determined using betaendorphinELISA kit. Serum beta-endorphin levels for groups 1, 2 and 3 were determined as157.0±23.1, 262.0±35.8, 574.3±95.4 pg/mL, respectively. There was significant differences in theserum beta-endorphin levels before, during and after slaughter. As a result, it has been revealedthat halal slaughter provides relief in animals instead of pain through a beta-endorphin reaction.
___
- Antalyalı A. Avrupa Birliği ve Türkiye’de Hayvan Refahı
Uygulamaları. AB Uzmanlık Tezi, Ankara: Avrupa Birliği
ve Diş İlişkiler Genel Müdürlüğü, 2007.
- Nakyinsige K, Che Man YB, Zeiad AA, et al. Stunning and
animal welfare from Islamic and scientific perspectives.
Meat Sci 2013; 95: 352-361.
- Nanda AS, Dobson H, Ward WR, Opioid modulation of
the hypothalamo-pituitary-adrenal axis in dairy cows.
Domest Anim Endocrinol 1992; 9:181-186.
- Shaw FD, Tume RK, The assessment of pre-slaughter
and slaughter treatments of livestock by measurement of
plasma constituents. Meat Sci 1992; 32:329-331.
- Şanlı Y, Veteriner Klinik Farmakoloji. 3. Baskı, İstanbul:
Özkan Matbaacılık, 1999.
- Kayaalp O. Tıbbi Farmakoloji. 12. Baskı, Ankara: Pelikan
Yayıncılık, 2009.
- Akalın PP, Başpınar N, Bucak MN, et al. Hayvanlarda
beta-endorfinin üreme sistemi ile ilişkisi. Journal of
Faculty of Veterinary Medicine, Erciyes University 2011;
8: 53-61.
- Jevremovic M, Terzic M, Kartaljevic G, et al. The
determination of immunoreactive beta–endorphin
concentration in the human fetal and neonatal thymus.
Horm Metab Res 1991; 23: 623-624.
- Angelogianni P, Li HL, Gianoulakis C, Ontogenesis of
proopiomelanocortin and its processing to ßendorphin by
the fetal and neonatal rat brain. Neuroendocrinology
2000; 72: 231-241.
- Micera E, Dimatteo S, Grimaldi M, et al. Stress indicators
in steers at slaughtering. Ital J Anim Sci 2007; 6: 457-459.
- Dereli Fidan E, Welfare Applications Related to
Livestocks in Turkey. Animal Health, Production and
Hygene 2012; 1: 39-46.
- Micera E, Albrizio M, Surdo NC, et al. Stress-related
hormones in horses before and after stunning by captive
bolt gun. Meat Sci 2010; 84: 634-637.
- Tume RK, Shaw FD, Beta-endorphin and cortisol
concentrations in plasma of blood samples collected
during exsanguination of cattle. Meat Sci 1992; 31: 211-
217.
- Djurović D, Milić-Askrabić J, Majkić-Singh N, Serum betaendorphin
level in patients with depression on
fluvoxamine. Farmaco1999; 54: 130-133.