Koç Spermasının Kısa Süreli Saklanabilirliği Üzerine L-Arjinin İlavesinin Etkisi

Bu çalışma koç spermasına L-arjinin ilavesinin kısa süreli saklamaya etkisini araştırmak amacıyla yapıldı. Altı koçtan 5 hafta boyunca haftada bir kez suni vajenle alınan ejekulatlar birleştirildi. Sperma örnekleri mililitrede 400 milyon motil sperm olacak şekilde Tris-yumurta sarısı sulandırıcısıyla sulandırıldıktan sonra 6 eşit kısma ayrıldı. Örneklerden biri kontrol amacıyla tutulurken, diğerlerine sırasıyla 0.1 mM, 0.5 mM, 1 mM, 5 mM, 10 mM L-arjinin ilave edildi. Örnekler kısa süreli saklama için soğutma kabininde +4 ?C'de bekletildi. 0., 12., 24. saatlerde ve daha sonra da 24 saat aralıklarla motilite, membran bütünlüğü, arginaz aktivite analizleri yapıldı. Kısa süreli saklanan örnekler değerlendirildiğinde, 10 mM L-arjinin ilavesinin sperma örneklerinin motilitesini 12. saatten sonra, membran bütünlüğünü 48. saatten sonra (P

Effect of L-Arginine Addition on Short Term Storability of Ram Semen

This study was conducted to investigate the effect of L-arginine addition on short term storability of ram semen. The semen collected from 6 Akkaraman rams by using artificial vagina once a weeks during 5 weeks, was pooled. The semen samples were diluted with Tris-egg yolk extender to include 400 million motile sperms per mL and were then divided into 6 equal groups. While group 1 was kept as control, 0.1 mM, 0.5 mM, 1 mM, 5 mM, 10 mM L-arginine were added to the other groups. The groups were kept at +4 ?C for short term storage. Motility, membrane integrity, arginase activity were analyzed at 0, 12th, 24th h and then at 24 h intervals. When the samples stored in short term at +4 ?C were evaluated, it was observed that the addition of 10mM L-arginine to semen samples decreased the motility after the 12nd h and membrane integrity after the 48th h (P

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