Ömer KIZIL, Engin BALIKCI, Abdullah GAZİOĞLU

Akut Ruminal Laktik Asidozisli Koyunlarda Akut Faz Protein Yanıtı

Bu çalışma akut laktik asidozisli koyunlarda pozitif akut faz proteinlerindeki değişimi belirlemek amacıyla yapılmıştır. Araştırmada 10 adet rumen asidozisli ve 10 adet sağlıklı koyundan oluşan toplam 20 adet koyun kullanılmıştır. Çalışmadaki tüm koyunların v. jugularis'lerinden kan örneği alınmış ve kısa sürede analizleri yapılmıştır. Serum amiloid A (SAA), Haptoglobin (Hp) ve C-reaktif protein (CRP) gibi akut faz proteinlerinin düzeyleri ELISA ile uygun ticari kitler kullanılarak belirlenmiştir. SAA ve Hp düzeyleri bakımından akut laktik asidozisli grupla kontroller arasında önemli farklılıklar (P

Acute Phase Protein Response in the Sheep with Acute Ruminal Lactic Acidosis

This study was conducted to determine the change in the positive acute phase proteins in sheep with acute ruminal lactic acidosis. The study was performed on 20 sheep consisting of 10 sheep with ruminal acidosis and 10 healthy controls. Blood samples were taken from the jugular vein of the animals and analyzed as soon as possible. The levels of acute phase proteins such as serum amyloid A (SAA), haptoglobulin (Hp) and C-reactive protein (CRP) were determined by ELISA using appropriate commercial test kits. Although the significant differences (P

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1. Braun U, Tihs R, Schefer U. Ruminal lactic acidosis in sheep and goats. Vet Rec 1992; 130: 343-349.
2. Beauchemin KA, Yang WZ, Rode LM. Effects of barley grain processing on the site and extent of digestion of beef feedlot finishing diets. J Anim Sci 2001; 79: 1925-1936.
3. Gentile A, Sconza S, Lorenz I. D-lactic acidosis in calves as a consequence of experimentally induced ruminal acidosis. J Vet Med Series A 2004; 51: 64-70.
4. Owens FN, Secrist DS, Hill WJ, Gill DR. Acidosis in cattle: A review. J Anim Sci 1998; 76: 275-286.
5. Nikolov Y. Biochemical alterations in rumen liquor, blood, cerebrospinal fluid and urine in experimental acute ruminal lactic acidosis in sheep. Indian Vet J 2003; 80: 36-39.
6. Constable PD. Grain overload in ruminants. In: Khan C (Editor). The Merck Veterinary Manual, 10 th edition. Merck & C Inc, Whitehouse, USA, 2010.
7. Kujala M, Orro T, Soveri T. Serum acute phase proteins as a marker of inflammation in dairy cattle with hoof diseases. Vet Rec 2010; 166: 240-241.
8. Ceciliani F, Ceron JJ, Eckersall PD, Sauerwein H. Acute phase proteins in ruminants. J Proteom 2012; 75: 4207- 4231.
9. McGrotty YL, Knottenbelt RIK, Reid AWJ, Eckersall PD. Haptoglobin in a canine hospital population. Vet Rec 2003; 152: 562-564.
10. Cray C, Zaias J, Altman NH. Acute Phase response in animals: A review. Comp Med 2009; 59: 517-526.
11. Eckersall PD, Conner JG. Bovine and canine acute phase proteins. Vet Res Commun 1988; 12: 169-178.
12. Murata H, Shimada N, Yoshioka M. Current research on acute phase proteins in veterinary diagnosis: An overview. Vet J 2004; 168: 28-40.
13. Eckersall P, Lawson F, Bence L, et all. Acute phase protein response in an experimental model of ovine caseous lymphadenitis. BMC Vet Res 2007; 3: 1-6.
14. Jain S, Gautam V, Naseem S. Acute-phase proteins: A diagnostic tool. J Pharmacy Bioallied Sci 2011; 3: 118-127.
15. Ulutas P, Özpınar A. Effect of Mannheimia (Pasteurella) haemolytica infection on acute-phase proteins and some mineral levels in colostrum-breast milkfed or colostrum- breast milk-deprived sheep. Vet Res Commun 2006; 30: 485-495.
16. Pfeffer A, Rogers KM, O'Keeffe L, Osborn PJ. Acute phase protein response, food intake, liveweight change and lesions following intrathoracic injection of yeast in sheep, Res Vet Sci 1993; 55: 300-306.
17. Skinner JG, Roberts L. Haptoglobin as an indicator of infection in sheep. Vet Rec 1994; 134: 33-36.
18. Horadogada NU, Knox KMG, Gibbs HA, et al. Acute phase proteins in cattle discrimination between acute and chronic inflammation. Vet Rec 1999; 144: 437-441.
19. Orro T, Jacobsen S, LePage JP, et al. Temporal changes in serum concentrations of acute phase proteins in newborn dairy calves. Vet J 2008; 176: 182-187.
20. Petersen HH, Nielsen JP, Heegaard PMH. Application of acute phase protein measurements in veterinary clinical chemistry. Vet Res 2004; 35: 163-187.
21. Tothova S, Nagy O, Kovac G. Acute phase proteins and their use in the diagnosis of diseases in ruminants: A review. Vet Med 2014; 59: 163-180.
22. Gruys E, Toussant MJM, Niewold TA, et al. Acute phase reaction and acute phase proteins. J Zheijang Univ Sci 2005; 6: 1045-1056.
23. Ceciliani F, Giordano A, Spagnolo V. The systemic reaction during inflammation: The acute-phase proteins. Prot Pept Lett 2002; 9: 211-223.
24. Heegaard PM, Godson D, Toussaint M, et al. The acute phase response of haptoglobin and serum amyloid A (SAA) in cattle undergoing experimental infection with bovine respiratory syncytial virus. Vet Immunol Immunopathol 2000; 77: 9-15.
25. Nagaraja TG, Bartley EE, Fina LR, Antony HD. Relationship of rumen gram-negative bacteria and free endotoxin to lactic acidosis in cattle. J Anim Sci 1978; 47: 1329-1336.
26. Andersen PH, Bergelin B, Christensen KA. Effect of feeding regimen on concentration of free endotoxin in ruminal fluid of cattle. J Anim Sci 1994; 72: 487-491.
27. Enemark JM, Jorgensen RJ, Enemark P. Rumen acidosis with special emphasis on diagnostic aspects of subclinical rumen acidosis: A review. Vet Zootec 2002; 20: 16-29.
28. Kleen JL, Hooijer GA, Rehage J. Subacute ruminal acidosis (SARA): A review. J Vet Med A Physiol Pathol Clin Med 2003; 50: 406-414.
29. Andersen PH. Bovine endotoxicosis: Some aspects of relevance to production diseases. Acta Vet Scand. 2003; 98: 141-155.
30. Ametaj BN, Bradford BJ, Bobe G, et all. Strong relationship between mediators of the acute phase response and fatty liver in dairy cows. Can J Anim Sci 2005; 85: 165-175.
31. Baumann H, Gauldie J. The acute phase response. Immunol Today 1994; 15: 74-80.
32. Ceron JJ, Eckersall PD, Martinez-Subiela S. Acute phase proteins in dogs and cats: current knowledge and future perspectives, Vet Clin Pathol 2005; 34: 85-99.
33. Enemark JM. The monitoring, prevention and treatment of sub-acute ruminal acidosis (SARA): A review. Vet J 2008; 176: 32-43.
34. Cannizzo C, Gianesella M, Guidice E, et al. Serum acute phase proteins in cows with SARA (Subacute Ruminal Acidosis) suspect. Arq Bras Med Vet Zootec 2012; 64: 15- 22.
35. Gonzalez FHD, Tecles F, Martinez-Subiela S. Acute phase protein response in goats. J Vet Diag Invest 2008; 20: 580- 584.
36. Lepherd M, Canfield P, Hunt G, Bosward K. Haematological, biochemical and selected acute phase protein reference intervals for weaned female Merino lambs. Aust Vet J 2009; 87: 5-11.
37. Eckersall PD, Lawson FP, Bence L. Acute phase protein response in an experimental model of ovine caseous lymphadenitis. BMC Vet Res 2007; 3: 35-40.
38. Chalmeh A, Badiei K, Poujafar M, Nazifi S. Acute phase response in experimentally Escherichia coli serotype O55:B5 induced endotoxemia and its comparative treatment with dexamethasone and flunixin in Iranian fattailed sheep. Vet Arhiv 2013; 83: 301-312.
39. El-Deeb WM. Novel biomarkers for pregnancy toxemia in ewes: Acute phase proteins and pro-inflammatory cytokines. Scientific Rep 2012; 1: 243-246.
40. Gonzalez FHD, Hernandez F, Madrid J, et al. Acute phase proteins in experimentally induced pregnancy toxemia in goats. J Vet Diagn Invest 2011; 23: 57-62.
41. Hashemnia M, Khodakaram-Tafti A, Razavi SM, Nazifi S. Changing patterns of acute phase proteins and inflammatory mediators in experimental caprine coccidiosis. Korean J Parasitol 2011; 49: 213-219.
42. Tothova C, Nagy O, Kovac G. Acute phase proteins and their use in the diagnosis of diseases in ruminants: A review. Vet Med 2014; 59: 163-180.
43. Gonzalez FHD, Ruiperez FH, Sanchez JM. Haptoglobin and serum amyloid A in subacute ruminal acidosis in goats. Rev Med Vet Zootec 2010; 57: 159-167.