Atilla YILDIZ, Engin BALIKÇI, Fuat GÜRDOĞAN

Effect of Injection of Vitamin E and Selenium Administered Immediately Before the Ovsynch Synchronization on Conception Rate, Antioxidant Activity and Progesterone Levels in Dairy Cows

Bu çalışmanın amacı, ovsynch senkronizasyonundan hemen önce tek doz derialtı uygulanan vitamin E ve selenyumun, laktasyondaki sütçü ineklerin gebe kalma oranları, antioksidan aktiviteleri ve plazma progesteron düzeyleri üzerine etkisini araştırmaktı. Yirmi dört sağlıklı inek rastgele deney (grup E, n=14) ve kontrol (grup C, n=10) grubuna ayrıldı ve ovsynch protokolüyle senkronize edildi. Grup Ede ineklere ovsynch senkronizasyonundan hemen önce tek doz 8 mL Esegal (60 mg/mL vitamin E and 1 mg/mL sodium selenite) derialtı yolla verildi. Grup Cdeki ineklere uygulama yapılmadı. Her iki gruptaki ineklerden tohumlamadan hemen önce ve 14 gün sonra kan örnekleri alındı. Gebelik teşhisi tohumlamadan 60 gün sonra yapıldı. Gruplar arasında gebe kalma oranlarında önemli farklılık bulunmadı. Tohumlamadan sonraki 14. günde grup Ede ortalama glutathione peroxidase (GSH-Px) ve superoxide dismutase (SOD) düzeylerinin grup Cdekine göre ve uygulama öncesi kendi düzeylerinden daha yüksek olduğu (P

Ovsynch Senkronizasyonundan Hemen Önce Vitamin E ve Selenyum Enjeksiyonunun Sütçü İneklerde Gebe Kalma Oranı, Antioksidant Aktivite ve Progesteron Düzeyleri Üzerine Etkisi

The objective of this study was to investigate the effect of a single subcutaneous injection of vitamin E and selenium administered immediately before ovsynch on conception rates, antioxidant and plasma progesterone levels of lactating dairy cows. Twenty four healthy cows were randomly distributed into the experimental group (group E, n=14) or the control group (group C, n=10) and synchronized with the ovsynch protocol. In group E, cows received a single subcutaneous injection of 8 mL Esegal (60 mg/mL vitamin E and 1 mg/mL sodium selenite) immediately before ovsynch. In group C, cows did not receive any injection. Blood samples were taken immediately before application and on day 14 after artificial insemination (AI). Pregnancy detection was performed on day 60 after AI. There were no significant differences in conception rates between the groups. The mean erythrocyte GSH-Px and SOD activities in group E on day 14 after AI were higher than group C and their own activities prior to application. The progesterone levels increased in group E compared to group C on day 14 after AI. In conclusion, a single subcutaneous injection of vitamin E and selenium immediately before ovsynch had no a significant effect on conception rates of lactating dairy cows. However, this application had a beneficial effect on erythrocyte GSH-Px and SOD activities and plasma progesterone levels.

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