Deneysel Olarak Metotreksat Uygulanmış Ratlarda Oksidatif Stres ve Antioksidan Enzim Aktiviteleri Üzerine Enginar Uygulamasının Etkisi

Çalışmada, metotreksat (MTX) uygulanmış ratların kan dokusunda oksidatif stres ve antioksidanenzim aktiviteleri üzerine enginar uygulamasının etkisini araştırmak amaçlanmıştır. Wistar-Albinoerkek ratlar; kontrol grubu, enginar uygulanan grup, MTX uygulanan grup ve MTX+enginaruygulanan grup şeklinde dört gruba ayrılmıştır. MTX ve enginar uygulamalarına aynı andabaşlanmıştır. Enginar 1.5 g/kg/gün vücut ağırlığı dozunda gavaj yoluyla 9 gün süre ile uygulanmış,MTX ise 7 mg/kg/gün vücut ağırlığı dozunda intraperitoneal olarak 3 gün uygulanmıştır. Dokuzgünlük deney periyodunun sonunda tüm ratlar sakrifiye edilmiş ve kan dokusu örnekleriantikoagulant içeren tüplere alınmıştır. Çalışmada, enginarın MTX kaynaklı hasar üzerindekietkilerini belirlemek amacıyla malondialdehid (MDA), redükte glutatyon (GSH) düzeyleri, katalaz(KAT), glutatyon peroksidaz (GSH-Px) ve süperoksit dismutaz (SOD) aktiviteleri ölçülmüştür. MTXuygulanan grupta kontrol grubu ile karşılaştırıldığında MDA (P

Influence of Artichoke Administration on Oxidative Stress and Antioxidant Enzyme Activities in Experimentally Methotrexate Administered Rats

In this study, it was aimed to investigate the effect of artichoke administration on oxidative stress and antioxidant enzyme activities in blood tissue of methotrexate (MTX) administered rats. Male Wistar-Albino rats were divided into four groups as described; control group, artichoke treated group, MTX treated group and MTX+artichoke treated group. MTX and artichoke applications were started at same time. Artichoke was applied at a dose of 1.5 g/kg/day body weight for 9 days by gavage, while MTX was administered intraperitoneally at a dose of 7 mg/kg/day for 3 days. At the end of the 9-day experiment period, all animals were sacrificed and blood tissue samples were removed into anticoagulant containing tubes. In this study, malondialdehyde (MDA), reduced glutathione (GSH) levels, catalase (CAT), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities were measured to determine the effects of artichoke on MTX-induced damage. In MTX treated group, MDA levels (P

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