An Immunohıstochemıcal Study on the Endocrıne Cells ın the Stomach and Intestıne Regıons of the Dıcentrarchus labrax, L.,1758

Dicentrarchus labrax’ ın mide ve bağırsak bölümlerindeki endokrin hücrelerin dağılım ve yoğunlukları bombesin, kolesistokinin (CCK)-8, histamin, neurotensin, sekretin, somatostatin-14, Trk A, Trk B, Trk C and vazoaktif intestinal polipeptid (VIP)’ e karşı hazırlanmış antiserum kullanılarak imunohistokimyasal metodlar ile araştırıldı. İmmunohistokimyasal çalışmalar sonucunda, balıkların mide ve bağırsak bölümlerinde çalışılan immunoreaktif hücrelerin dağılım ve yoğunluklarında farklılık olduğu gözlendi. Fundus ve pilorus bölgelerinde histamin, Trk B ve Trk C immunoreaktif hücreler gözlendi. Anterior ve posterior bağırsak bölgelerinde Trk A immunoreaktif hücrelerin bulunduğu belirlendi. CCK-8 ve somatostatin-14’ e reaktif hücreler sadece anterior bağırsakta gösterildi.

Dıcentrarchus labrax, L., 1758 Mide ve Bağırsak Bölgelerindeki Endokrin Hücreler Üzerine İmmunohistokimyasal Çalışma

The distribution and relative frequency of endocrine cells was studied in the stomach and intestine regions of the Dicentrarchus labrax, by immunohistochemistry using antisera against bombesin, cholecystokinin (CCK)-8, histamine, neurotensin, secretin, somatostatin-14, Trk A, Trk B, Trk C and vasoactive intestinal peptide (VIP). As a result of immunohistochemical observations, immunoreactive cells studied were detected to be localized in different distribution and relative frequency in the stomach and intestine regions of Dicentrarchus labrax. Histamine, Trk B and Trk C immunoreactive cells were detected in the fundus and pylorus regions. Trk A immunoreactive cells were found in the anterior and posterior intestine. Cells reactive for CCK-8 and somatostatin-14 were demonstrated only in the anterior intestine.

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