Ibrahim ADESHINA, Mozeedah ABDULWAHAB, Yusuf Adetunji ADEWALE, Lateef Oloyede TIAMIYU

Afrika kedibalığı (Heterobranchus bidorsalis) için Antimikrobiyal Ajanlar olarak Tridax procumbens Yaprak Özü Potansiyelleri

Afrika kedibalığı (Heterobranchus bidorsalis) için Antimikrobiyal Ajanlar olarak Tridax procumbens Yaprak Özü Potansiyelleri Balıklara olan talebin artması, yeni balık hastalıklarının ortaya çıkışı ve ortaya çıkışı ile eski balık yetiştiriciliğinin tekrar yaşanmasına neden olan yoğun akuakültür uygulamalarına da yol açmıştır. Doğal ve çevre dostu materyalleri kullanarak farmasötik ilaçlara alternatif terapi yapılmasının savunulması bu çalışmayı gerektirdi. Tridax procumbens özütlerinin antimikrobiyal özellikleri, patojenik balık bakterilerine (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aereus, Aeromonashydrophilia ve Listeria monocytogenes) karşı test edilmiştir. Bakteriler kültürlendi, minimum inhibisyon konsantrasyonu (MIC) ve inhibisyon zonu (ZI) standart yöntemler kullanılarak gerçekleştirildi. Yüz sekiz (108) Hetrobranchus bidorsalis yavruya 54 gün süreyle tamamen randomize edilmiş olarak günde iki kez T. procumbens esaslı diyetler (% 40 ham protein) verilmiştir. Elde edilen veriler, betimsel istatistikler ve ANOVA kullanılarak ?0.05'de analiz edilmiştir. Elde edilen sonuçlar, T. procumbens ekstraktının alkaloidler, flavonoidler, taninler, steroidler ve karptenoidler içerdiğini, ekstraktaki saponin ve terpoidlerin bulunmadığını göstermektedir. Etkili MİK 40 mg / ml idi ve ZI, 12.85 ± 0.39 ila 14.20 ± 0.74 mm arasında değişiyordu. Patojenler L. monocyogenes hariç T. procumbens ekstraktına duyarlıydı. TVC ve TCC, tedavi edilen grupta anlamlı olarak azaldı. Sonuçlar, T. procumbens yapraklarının, su ürünleri yetiştiriciliğinde potansiyel kullanımını göstermiş olabilecek antimikrobiyal özelliklere sahip olduğunu ortaya koymuştur. Ayrıca, T. procumbens'in test edilen organizmalara karşı antimikrobiyal etkinliğe sahip olduğunu ve balık sağlığı yönetiminde kullanılabileceğini belirtti.

Potentials of Tridax procumbens Leaf Extract as Antimicrobial Agents for African catfish (Heterobranchus bidorsalis

The increase in the demand for fish has led to intensive aquaculture practices which has also ushered in a manifestation and emergence of new fish diseases and reoccurrence of old ones. The advocacy for alternative therapy to drugs using natural and environmental friendly materials necessitated this study. The antimicrobial properties of Tridax procumbens extracts were tested against pathogenic fish bacteria (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aereus, Aeromonashydrophilia, and Listeria monocytogenes). Bacteria were cultured, minimum inhibitory concentration (MIC) and zone of inhibition (ZI) were carried out using standard methods. One hundred and eight (108) Hetrobranchus bidorsalis juveniles were fed T. procumbens based diets (40% crude protein) two times daily in a completely randomized designed for 54 days. Data obtained were analyzed using descriptive statistics and ANOVA at ?0.05. The results shows that T. procumbens extract contained alkaloids, flavonoids, tannins, steroids and carptenoids while saponin and terpnoids were not detected in the extract. Effective MIC was 40mg/ml and ZI ranged from 12.85±0.39 to 14.20±0.74 mm. Pathogens were sensitive to the T. procumbens extract except for L. monocyogenes. The TVC and TCC were significantly reduced in the treated group. The results revealed T. procumbens leaves have antimicrobial properties which may had shown its potential utilization in aquaculture. It also indicated that T. procumbens have antimicrobial activity against tested organisms and could be used fish health management.

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