Sıçan Testis Dokusunda Kadmiyum ile Oluşturulan Hasar Üzerine Etil Pirüvatın Etkilerinin Araştırılması

Amaç: Bu çalışmada kadmiyum ile testis dokusunda oluşturulan apoptozis ve hasar üzerine etil pirüvatın koruyucu etkisinin araştırılması amaçlandı. Gereç ve Yöntem: Bu çalışmada 32 adet ergin erkek Wistar albino türü sıçanlar kullanıldı. Denekler rastgele 4gruba ayrıldı. Grup I; (n=8) kontrol grubu, Grup II; (n=8) 2,5 mg/kg intraperitoneal (i.p) kadmiyum uygulanan grup, Grup III; (n=8) 2,5 mg/kg kadmiyum + 100 mg/kg (i.p) etil piruvat uygulanan grup ve Grup IV; (n=8) 100 mg/kg (ip) etil piruvat uygulanarak oluşturuldu. Yirmi dört saat arayla iki doz etil piruvat uygulandı. İkinci uygulamasından bir saat sonra 2 ve 3. gruplara kadmiyum uygulandı ve uygulamadan 24 saat sonra denekler dekapite edilerek testis dokuları alındı. Bulgular: Kadmiyum uygulanan grupta germinalepitelde düzensizlik, epitel hücreleri arasında vakuol oluşumu ve yer yer nekrotik tübüller gözlendi. Kadmiyum uygulanan grupta seminifertübül çapları, Johnsen'intübüler biyopsi skoru ve doku MDA düzeylerinin kontrol ile karşılaştırıldığında anlamlı derecede azaldığı, apoptotik hücre sayısının ise anlamlı derecede arttığı belirlendi. Koruyucu amaçlı verilen etil pirüvatın sadece apoptotik hücre sayısında iyileşme sağladığı gözlendi. Sonuç: Sonuç olarak kadmiyum uygulamasının testis dokusunda çok ciddi histopatolojik değişiklikler oluşturmakta olduğu ve koruyucu amaçlı verilen etil pirüvatın bu hasarda etkili bir şekilde iyileştirici etkisinin olmadığı sadece apoptotik hücre sayısında iyileşme sağladığı gözlendi.

A Research on Effects of Ethyl Pyruvate on Rat Testical Tissues which Was Damaged with Cadmium

A Research on Effects of Ethyl Pyruvate on Rat Testical Tissues which Was Damaged with Cadmium Objective: The present study aimed to evaluate the protective effect of Ethyl Pyruvate on cadmium induced testicular apoptosis and toxicity in rats. Material and Method: In this study 32 adult male Wistar albino rats were used. Rats are seperated into four random groups. Group 1; (n=8) control group, Group 2; (n=8) 2.5 mg/kg intraperitoneal (ip) cadmium administered rats, Group 3; (n=8) 2.5 mg/kg ip cadmium + 100 mg/kg ethyl pyruvate administered rats, Group 4; (n=8) 100 mg/kg ethyl pyruvate administered rats. Two doses of the ethyl pyruvate were administered in a 24 hours interval. Cadmium was applied 1 hour after administration of ethyl pyruvate to the groups 2 and 3 and all the subjects are killed by decapitation 24 hour after and their testis tissues were collected.Results: Germinal epithelium irregularities, epithelial cell loss in lumen, formation of the vacuoles between epithelial cells and necrotic tubules were observedin the cadmium applied group. The diameters of seminiferous tubules, tubular biopsy score of Johnsen and tissue MDA levels significantly decreased and apoptotic cell numbers significantly increased compared to control group. It was observed that ethyl pyruvate has only shown improvement in the number of apoptotic cells. Conclusion: As a result application of cadmium create very serious changes on testicular tissue and the ethyl pyruvate administration for protective purposes is not preventing this damage except improvement in the number of apoptotic cells.

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1. Leoni G, Bogliolo L, Deiana G, et al. Influence of cadmium exposure on in vitroovine gamete dysfunction. ReprodToxicol 2002; 16: 371-377.
2. Tekelioğlu M. Özel Histoloji, İnce Yapı ve Gelişme, Erkek Üreme Sistemi. Ankara Üniversitesi Tıp Fakültesi Yayınları, Ankara 2002; 231-244.
3. Sab Dart ID. Committee Draft, Evidence um Developmental and Reproductive Toxicity of Cadmium. Reproductive and Cancer Hazard Assessment Section (RCHAS), 1996.
4. Rencüzoğulları N. Ratlarda Deneysel Olarak Oluşturulan Kadmiyum Toksikasyonu Üzerine Likopenin Etkilerinin Araştırılması, Yüksek Lisans Tezi, Mkü Sağ Bil Estitüsü, 2006.
5. Schwitters B, Masquellier J. OPC in Practice: Bioflavanols and Their Application. Alfa Omega Rome, Italy 1993.
6. Damek M, Poprawa A, Kapustab SK. Histopathological Changes in the Liver, Kidneys, and Testes of Bank Voles Environmentally Exposed to Heavy Metal Emissions from theS teel Works and Zinc Smelter in Poland. Environmental Research 2004; 96: 72-8.
7. Hew KW, Heath GL, Jiwa AH, et al. Cadmium in Vivo Causes Distruption of Tight Junction-Associated Microfilaments in Rat Sertoli Cells. Biol Reprod 1993; 49: 840-9.
8. ChungKy, Park JJ, Kim YS. The Role of High-Mobility Group Box-1 In Renal Ischemia And Reperfusion Injury And The Effect Of Ethyl Pyruvate. Transplant Proc 2008; 40: 2136-8.
9. Undurti N. Das,Is Pyruvate An Endogenous Anti-Inflammatory Molecule? Nutrition 2006; 22: 965-72.
10. Uchiyama T, Delude RL, Fink MP. Dose-Dependent Effects Of Ethyl Pyruvate In Mice Subjected to Mesenteric Ischemia and Reperfusion. Intensive Care Med 2003; 29: 2050-8.
11. Undurti N. Das Pyruvate Is An Endogenous Anti-Inflammatory And Anti-Oxidant Molecule. Med Sci Monit 2006; 12: 79-84.
12. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95: 351-8.
13. Baldwin DR, Marshall WJ. Heavy Metal Poisoning and It's Laboratory Investigation. Ann Clin Biochem. 1999; 36: 267-300.
14. Agency for Toxic Substances and Disease Registry (Atsdr), Toxicological Profile For Cadmium, Draft for Public Comment Update). PublicHealth Service, Us Depertmant of Health and Human Service, 1997.
15. Karataş S. Sıçanlarda Kadmiyum Klorürün (Kadmiyumcl2) Testis Dokusuna Etkisi, Yüksek Lisans Tezi, Eskişehir Osmangazi Üniversitesi, Sağlık Bilimleri Enstitüsü, Histoloji ve Embriyoloji Anabilim Dalı, 1998.
16. Suzukı Y. Cadmium Metabolism and Toxicity in Rats After Long-Term Subcutaneous Administration. J.Toxicol. Environ. Health 1980; 6: 469-82.
17. Davıson AG, Newman AJ, Taylor JD et al. Cadmium Fume Inhalation and Emphysema, Lancet 1988; 26: 663-7.
18. Gavett SH, Oberdorster G. Cadmium Chloride and Cadmium Metallotione in-Induced Pulmonary Injury and Recruitment of Polymorphonuclear Leukocytes.Exp. Lung. Res 1994; 20: 517- 37.
19. Gouveia MA. The Testes in Cadmium Intoxication: Morphological and Vascular Aspects. Andrologia 1988; 20: 225- 31.
20. Boscolo P, Sacchettonı-Logroscıno G, Ranellettı FO, et al. Effects of Long Term Cadmium Exposure on the Testis of Rabbits, Ultrastructural Study. Toxicoll Lett 1985; 24: 145-9.
21. Aktas C, Kanter M, Erboga M, Ozturk S. Anti-ApoptoticEffects of Curcumin on Cadmium-Induced Apoptosis In Rat Testes. Toxicol Ind Health 2012; 28: 122-30.
22. Marmar JL, Benoff S. The Safety of Ultrasonically Guided Testis Aspiration Biopsies and Efficacy of Useto Predict Varicocelectomy Outcome. Human Reproduction 2005; 20: 2279-88.
23. Wang W, Sun Y, Liu J, et al. ProtectiveEffect of the aflavins on Cadmium- Induced Testicular Toxicity in Male Rats. Food And Chemical Toxicology 2012; 50: 3243-50.
24. Ren XM, Wang G, Xu D,et al. The Protection of Selenium on Cadmium-Induced Inhibition of Spermatogenesis Via Activating Testosterone Synthesis In Mice. Food Chem. Toxicol 2012; 50: 3521-9.
25. Li JL, Gao R, Li S, et al. Testicular Toxicity Induced by Dietary Cadmium in Cocks And Ameliorative Effect by Selenium 2010; 23: 695-705.
26. Ji YL Wang H, Meng C, et al. Melatonin Alleviates CadmiumInduced Cellular Stress And Germ Cell Apoptosis In Testes. J. Pineal Res 2012; 52: 71-9.
27. Salahudeen AK, Clark EC, Nath KA. Hydrogen Peroxide Induced Renal Injury. A Protective Role for Pyruvate in Vitro and in Vivo. J Clin Invest 1991; 88: 1886-93.
28. Cicalese L, Lee K, Schraut W, Watkins S, Borle A, Stanko R. Pyruvate Prevents Ischemia Reperfusion Mucosal Injury of Rat Small Intestine. Am J Surg 1999; 171: 97-100.
29. Bunger R, Mallet RT, Hartman DA. Pyruvate Enhanced Phosphorylation Potential and Inotropism in Normoxic and Postischemic Isolated working Heart: Near-Complete Preventon of Reperfusion Contractile Failure. Eur J Biochem 1989; 180:221- 33.
30. Sileri P, Schena S, Morini S, et al. Pyruvate Inhibits Hepatic Ischemia- Reperfusion Injury In Rats. Transplantation 2001; 72: 27-30.
31. Lee JY, Kim YH, Koh JY. Protection by Pyruvate Against Transient Forebrain Ischemia in Rats. J Neurosci 2002; 21: 1-6.
32. Zhao W, Devamanoharan PS, Henein M, et al. Diabetes-Induced Biochemical Changes in Rat Lens: Attenuat Ion of Cataractogenesis By Pyruvate. Diabetes Obes Metab 2000; 2: 165-74.
33. Slovin PN, Huang CJ, Cade JR, et al. Sodium Pyruvate Is Better Than Sodium Chloride as a Resuscitat Ion Solution in a Rodent Model of Profound Hemorrhagic Shock. Resuscitation 2001; 50: 109-15.
34. Mongan PD, Capacchione J, Fontana JL, et al.Pyruvate Improves Cerebral Metabolism During Hemorrhagic Shock. Am J Physiol 2001; 281: 854-64.
35. Mongan PD, Fontana JL, Chen R, et al. Int Ravenous Pyruvate Prolongs Survival During Hemorrhagic Shock In Swine. Am J Physiol 1999; 277: 2253-63.
36. Varma SD, Devamanoharan PS, Rutzen AR, et al.Attenuation of Galactose-Induced Cataract by Pyruvate. Free Rad Res 1999; 30: 253-63.
37. Zhao W, Devamanoharan PS, Varma SD. Fructose Induced Deactivation of Ant Ioxidant Enzymes: Preventive Effect of Pyruvate. Free Rad Res 2000; 33: 23-30.
38. Payabvasha S, Kiumehra S, Tavangarb SM, et al. Ethyl Pyruvate Reduces Germ Cell-SpecificApoptosis and Oxidative Stress in Rat Model of Testicular Torsion/Detorsion. Journal Of Pediatric Surgery 2008; 43: 705-12