Serulein ile akut pankreatit oluşturulan ratlarda melatoninin koagulasyon parametreleri üzerine etkileri

Amaç: Bu çalışmanın amacı cerulein ile akut pankreatit oluşturulan ratlarda melatoninin koagulasyon parametreleri üzerine olası etkilerini değerlendirmektir. Gereç ve Yöntem: Bu amaçla 32 yetişkin erkek sağlıklı Wistar Abino rat kullanıldı. Hayvanlar dört gruba ayrıldı. Grup 1’deki hayvanlara herhangi bir uygulama yapılmadı. Grup II’deki hayvanlara iki saat aralıkla iki kez 50 mg/kg melatonin intraperitoneal olarak uygulandı. Grup III’deki hayvanlara iki saat aralıkla iki intraperitoneal serulein (sırasıyla, 50 μg/kg ve 25 μg/kg bw) enjeksiyonu yapıldı. Grup IV’deki hayvanlara iki saat aralıkla iki intraperitoneal serulein (sırasıyla, 50 μg/kg ve 25 μg/kg bw) enjeksiyonu yapıldı ve ratlara her bir serulein enjeksiyonundan 30 dk önce intraperitoneal olarak 50 mg/kg melatonin enjeksiyonu yapıldı. Hayvanlardan alınan kan örneklerinde platelet, fibrinojen, APTT, PT, INR düzeyleri belirlendi. Bulgular: Platelet sayısı ve fibrinojen seviyesi akut pankreatite bağlı olarak kontrol grubu ile karşılaştırıldığında arttı (p

The effect of melatonin on coagulation parameters in rats with cerulein-induced acute pancreatitis

Aim: The aim of this study was to evaluate possible effectsof melatonin on coagulation parameters in rats with ceruleininduced acute pancreatitis.Materials and Methods: For this purpose, 32 adult, male,healthy Wistar Abino rats were used. The animals were dividedinto four groups. Group I animals was no applied. GroupII animals was intraperitoneally administered 50 mg/kg melatoninper rat twice for two hours intervals. Animals of groupIII received two intraperitoneal injections of cerulein (50μg/kg and 25 μg/kg bw, respectively) at two hours intervals.Animals of group IV received two intraperitoneal injectionsof cerulein (50 μg/kg and 25 μg/kg bw, respectively) at twohours intervals and the rats received an intraperitoneal injectionof 50 mg/kg melatonin 30 min before each ceruleininjection. In blood samples taken from all animals, platelet,fibrinogen, activated partial thromboplastin time (APTT),prothrombin time (PT), international normalized ratio (INR)levels were determined.Results: Acute pancreatitis caused increment in platelet countand fibrinogen level compared to control group (p

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Agarwal N, Pitchumoni CS, 1993. Acute pancreatitis: a multisystem disease. Gastroenterologist, 1(2), 115-128.
Baddeley RNB, Skipworth JRA, Pereira SP, 2011. Acute pancreatitis. Medicine, 39(2), 108-115.
Bekyarova G, Tancheva S, Hristova M, 2010. The effects of melatonin on burn-induced inflammatory responses and coagulation disorders in rats. Methods Find Exp Clin Pharmacol, 32(5), 299-303.
Bockman DE, Büchler M, Beger HG, 1986. Ultrastructure of human acute pancreatitis. Int J Pancreatol, 1(2), 141-153.
Bouchard BA, Tracy PB, 2001. Platelets, leukocytes, and coagulation. Curr Opin Hematol, 8(5), 263-269.
Carrillo-Vico A, Guerrero JM, Lardone PJ, Reiter RJ, 2005. A review of the multiple actions of melatonin on the immune system. Endocrine, 27(2), 189-200.
Claustrat B, Brun J, Chazot G, 2005. The basic physiology and pathophysiology of melatonin. Sleep Med Rev, 9(1), 11-24.
Dahm A, Osterud B, Hjeltnes N, Sandset PM, Iversen PO, 2006. Opposite circadian rhythms in melatonin and tissue factor pathway inhibitor type 1: does daylight affect coagulation?. J Thromb Haemost, 4(8), 1840-1842.
Dhainaut JF, Marin N, Mignon A, Vinsonneau C, 2001. Hepatic response to sepsis: Interaction between coagulation and inflammatory processes. Crit Care Med, 29(7), 42-47.
Esmon CT, 1999. Possible involvement of cytokines in diffuse intravascular coagulation and thrombosis. Baillieres Best Pract Res Clin Haematol, 12(3), 343-359.
Esmon CT, Taylor FB, Snow TR, 1991. Inflammation and coagulation: linked processes potentially regulated through a common pathway mediated by protein C. Thromb Haemost, 66(1), 160-165.
Förstermann U, Münzel T, 2006. Endothelial nitric oxide synthase in vascular disease: from marvel to menace. Circulation, 113(13), 1708-1714.
Goldacre MJ, Roberts SE, 2004. Hospital admission for acute pancreatitis in an English population, 1963-98: database study of incidence and mortality. BMJ, 328(7454), 1466- 1469.
Hackert T, Pfeil D, Hartwig W, Fritz S, Schneider L, Gebhard MM, Büchler MW, Werner J, 2007. Platelet Function in Acute Experimental Pancreatitis. J Gastrointest Surg, 11(4), 439-444.
Kakafika A, Papadopoulos V, Mimidis K, Mikhailidis DP, 2007. Coagulation, Platelets, and Acute Pancreatitis. Pancreas, 34(1), 15-20.
Lasson A, Ohlsson K, 1986. Consumptive coagulopathy, fibrinolysis and protease-antiprotease interactions during acute human pancreatitis. Thromb Res, 41(2), 167-183.
Levi M, Van Der Poll T, Büller HR, 2004. Bidirectional relation between inflammation and coagulation. Circulation, 109(22), 2698-2704.
Libby P, Theroux P, 2005. Pathophysiology of coronary artery disease. Circulation, 111(25), 3481-3488.
Lotufo CM, Lopes C, Dubocovich ML, Farsky SH, Markus RP, 2001. Melatonin and N-acetylserotonin inhibit leukocyte rolling and adhesion to rat microcirculation. Eur J Pharmacol, 430(2-3), 351-357.
Muller G, Goettsch C, Morawietz H, 2007. Oxidative stress and endothelial dysfunction. Hamostaseologie, 27(1), 5-12. Okajima K, 2000. SIRS and the coagulation of normality. Rinsho Byori, 48(6), 510-515.
Reiter RJ, 2000. Melatonin: Lowering the high price of free radicals. News Physiol Sci, 15(5), 246-250.
Reiter RJ, Tan DX, Manchester LC, Qi W, 2001. Biochemical reactivity of melatonin with reactive oxygen and nitrogen species: A review of the evidence. Cell Biochem Biophys, 34(2), 237-256.
Reiter RJ, Tan DX, Qi W, Manchester LC, Karbownik M, Calvo JR, 2000. Pharmacology and physiology of melatonin in the reduction of oxidative stress in vivo. Biol Signals Recept, 9(3-4), 160-171.
Rodriguez C, Mayo JC, Sainz RM, Antolín I, Herrera F, Martín V, Reiter RJ, 2004. Regulation of antioxidant enzymes: A significant role for melatonin. J Pineal Res, 36(1), 1-9.
Salomone T, Tosi P, Palareti G, Tomassetti P, Migliori M, Guariento A, Saieva C, Raiti C, Romboli M, Gullo L, 2003. Coagulative disorders in human acute pancreatitis: role for the D-dimer. Pancreas, 26(2), 111-116.
Shebuski RJ, Kilgore KS, 2002. Role of inflammatory mediators in thrombogenesis. J Pharmacol Exp Ther, 300(3), 729-735.
Stevenson K, Carter CR, 2013. Acute pancreatitis. Surgery, 31(6), 295-303.
Tunali T, Sener G, Yarat A, Emekli N, 2005. Melatonin reduces oxidative damage to skin and normalizes blood coagulation in a rat model of thermal injury. Life Sci, 76(11), 1259- 1265.
Viles-Gonzalez JF, Fuster V, Badimon JJ, 2006. Links between inflammation and thrombogenicity in atherosclerosis. Curr Mol Med, 6(5), 489-499.
Wang XD, Wang Q, Andersson R, Ihse I, 1996. Alterations in intestinal function in acute pancreatitis in an experimental model. Br J Surg, 83(11), 1537-1543.
Warzecha Z, Dembiński A, Ceranowicz P, Dembiński M, Cieszkowski J, Kuśnierz-Cabala B, Naskalski JW, Jaworek J, Konturek SJ, Pawlik WW, Tomaszewska R, 2007. Influence of ischemic preconditioning on blood coagulation, fibrinolytic activity and pancreatic repair in the course of caerulein- induced acute pancreatitis in rats. J Physiol Pharmacol, 58(2), 303-319.
Wirtz PH, Spillmann M, Bärtschi C, Ehlert U, von Känel R, 2008. Oral melatonin reduces blood coagulation activity: a placebo-controlled study in healthy young men. J Pineal Res, 44(2), 127-133.
Xenoulis PG, Suchodolski JS, Steiner JM, 2008. Chronic pancreatitis in dogs and cats. Compend Contin Educ Vet, 30(3), 166-180.