Akut kadmiyum toksikasyonu oluşturulan ratlarda quercetinin bazı kan parametreleri ile antioksidan sistem üzerine etkileri

Amaç: Bu çalışmada akut kadmiyum toksikasyonuna maruz kalan ratlarda quercetinin bazı kan parametreleri ile antioksidan sistem üzerine etkilerinin belirlenmesi amaçlanmıştır. Gereç ve Yöntem: Bu amaç doğrultusunda, canlı ağırlıkları birbirine yakın olarak seçilmiş 30 adet yetişkin erkek “Wistar- Albino” rat kullanıldı. Çalışmada kullanılan hayvanlar Kontrol grubu (K), Kadmiyum grubu (Cd), Quercetin grubu (Q) ve Kadmiyum + Quercetin grubu (CdQ) olmak üzere dört gruba ayrıldı. Cd grubunda yer alan ratlara 3 gün boyunca 4mg/kg/ canlı ağırlık/gün dozunda kadmiyum klorür (CdCl2) subkutan olarak uygulanırken, Q ve CdQ grubundaki ratlara ise 50 mg/ kg/canlı ağırlık/gün dozunda quercetin intraperitonal olarak yine 3 gün boyunca enjekte edildi. Kontrol grubunda yer alan ratlara herhangi bir uygulama yapılmadı. Kan örnekleri çalışmanın başlangıcından sonraki 4. günde toplandı. Bulgular: Deneme sonunda serum ve plazma örneklerinden SOD, MDA, GSH, katalaz, ALT, AST, GGT, total protein ve albümin düzeyleri belirlendi. Serum MDA düzeyinin sadece Cd uygulanan grupta önemli oranda yüksek olduğu belirlendi (P

The effects of quercetin on antioxidant system and some blood parameters in rats exposed to acute cadmium toxicity

Aim: The aim of this study was to determine the effects of quercetinon antioxidant system and some blood parameters inrats exposed to acute cadmium toxicity.Materials and Methods: Adult male “Wistar-Albino” rats (n= 30) were used and divided into four groups as Control (C, n= 6), Cadmium (Cd, n = 8), Quercetin (Q, n = 8) and Cadmium+ Quercetin (Cd + Q, n = 8). Cadmium chloride (CdCl2, 4 mg/kg daily, s.c) were administrated to Cd and Cd+Q groups, andQuercetin (Q, 50 mg/kg daily, i.p) were given to Q and Cd + Qgroups for 3 days, respectively. Control group was not recievedany treatment. Blood samples were collected from all animalsat fourth day after treatments.Results: The levels of serum SOD, MDA, GSH, catalase, andplasma ALT, AST, GGT, total protein, albumin were detected.Although, serum MDA levels were found higher (P< 0.05) inCd than the other groups, it was similiar in Q and C groups.ALT, AST and GGT enzyme levels were observed higher in Cdthan C and Q groups (P< 0.05).Conclusion: Negative effects of acute cadmium toxicity onantioxidan system and some hematological parameters wereameliorated with quercetin treatment in rats.

PDF

___

Andujar P, Bensefa-colas L, Descatha A, 2010. Acute and chronic cadmium poisoning. Rev Med Interne, 31(2), 107- 15. doi:10.1016/j.revmed.2009.02.029.
ATSDR, 2012. Public Health Statement for Cadmium, Agency for Toxic Substances and Disease Registry. http://www. atsdr.cdc.gov/PHS/PHS.asp?id=46&tid=15.
Bernhoft RA, 2013. Cadmium toxicity and treatment. Sci World J, 394652. Article ID 394652,7.
Bu T, Mi Y, Zeng W, Zhang C, 2013. Protective effect of quercetin on cadmium-induced oxidative toxicity on germ cells in male mice. Anat Rec, 294 (3), 520-26. doi:10.1002/ ar.21317.
Chen X, 2010. Protective effects of quercetin on liver injury induced by ethanol. Pharmacogn Mag, 6 (22), 135-41. doi:10.4103/0973-1296.62900.
Chlebda E, Magdalan J, Merwid-lad A, Trocha M, Kopacz M, Kuźniar A, 2010. Influence of water-soluble flavonoids, quercetin-5′-sulfonic acid sodium salt and morin-5′- sulfonic acid sodium salt, on antioxidant parameters in the subacute cadmium intoxication mouse model. Exp Toxicol Pathol, 62(2), 105-8,. doi:10.1016/j.etp.2009.02.118.
El-boshy ME, Risha EF, Abdelhamid FM, Mubarak MS, Hadda TB, 2014. Protective effects of selenium against cadmium induced hematological disturbances, immunosuppressive, oxidative stress and hepatorenal damage in rats. J Trace Elem Med Biol, 29, 104-10. doi:10.1016/j. jtemb.2014.05.009.
Erguzel ET, 2006. Quercetin (3, 3’, 4’, 5, 7-pentahidroksiflavon)’in Bakır (II) ve Çinko (II) Komplekslerin Kararlılık Sabitlerinin Tayini, Marmara Univ. Fahim MA, Nemmar A, Dhanasekaran S, Singh S, Shafiullah M, Yasin J, 2012. Acute cadmium exposure causes systemic and thromboembolic events in mice. Physiol. Res, 61(1) 73-80.
Fang C, 2007. Characterization of Polyphenol Oxidase and Antioxidants From Pawpaw ( Asimina Tribola) Fruit. University of Kentucky Master's Theses, 477.
Gencer M, Karaca T, Gungor A, 2014. The protective effect of quercetin on IMA levels and apoptosis in experimental ovarian ischemia-reperfusion injury. Eur J Obstet Gynecol Reprod Biol, 177, 135-40. doi: 10,1016/j.ejogrb.2014.03.036.
Gerhardsson LLU, Englyst V, Lundstrom NG, Sandberg S, Nordberg G, 2002. Cadmium, copper and zinc in tissues of deceased copper smelter workers. J Trace Elem Med Biol, 16(4), 261-66.
Gulcen B, Karaca O, Kus MA, Akpolat N, Kus I, 2011. The Protective Effects Of Melatonin Hormone Against Experimental Cadmium Toxicity In The Liver : An Immunohistochemical Study. Düzce Univ Sag Bil Enstit Derg, 1, 13-17.
Hounkpatin YAS, Edorh AP, Guedenon P, 2013. Haematological evaluation of Wistar rats exposed to chronic doses of cadmium, mercury and combined cadmium and mercury. Afr J Biotechnol, 12(23), 3731-37. doi:10.5897/ AJB12.2669.
Hussein SA, Abd-el-maksoud HA, Agag BI, El-nile MB, 2009. Effect of cadmium toxicity on some biochemical blood parameters and their modulation with certain natural antioxidants in rabbits. Benha Ras Surd, 338-62.
Kanter M, Unsal C, Aktas C, Erboga M, 2013. Neuroprotective effect of quercetin against oxidative damage and neuronal apoptosis caused by cadmium in hippocampus. Toxicol Ind Health, 32(3), 541-50. doi:10.1177/0748233713504810.
Karabulut-bulan O, Bolkent S, Yanardag R, 2008. The role of vitamin C, vitamin E, and selenium on cadmium-induced renal toxicity of rats. Drug Chem Toxicol, 31(4), 413-26.
Karabulut-bulan O, Koyuturk M, Bolkent S, Yanardag R, Tabakoglu A, 2004. Sıçan Tiroid Bezinde Kadmiyum Hasarına Karşı C Vitamini, E Vitamini Ve Selenyumun Kombine Kullanımının Etkileri. Cerrahpaşa Tıp Derg, 35(4), 174-80.
Kocak M, Akcil E, 2006. The effects of chronic cadmium toxicity on the hemostatic system. Pathophysiol Haemost Thromb, 35(6), 411-16.
Krishnakumar N, Prabu S.M, Sulfikkaralı NK, 2012. Quercetin protects against cadmium-induced biochemical and structural changes in rat liver revealed by FT-IR spectroscopy, Biomed Prevent Nutr, 2, 179-85. doi:10.1016/j.bionut. 2012.03.010.
Lee DH, Blomhoff R, Jacobs DR, 2004. Is serum gamma glutamyltransferase a marker of oxidative stress? Free Radical Res, 38(6), 535-39.
Marcolin É, Forgiarini LF, Rodrigues G, Tieppo J, Borghetti GS, Bassani VL, 2013. Quercetin Decreases Liver Damage in Mice with Non-Alcoholic Steatohepatitis. Basic Clin Pharmacol Toxicol, 112(6), 385-91. doi:10.1111/bcpt.12049.
Pavanato A, Tuñón MJ, Sánchez-campos S, Marroni CA, Llesuy S, González-Gallego J, Marroni N, 2003. Effects of quercetin on liver damage in rats with carbon tetrachloride-induced cirrhosis. Dig Dis Sci, 48(4), 824-29.
Pourmorad F, Hosseinimehr SJ, Shahabimajd N, 2006. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr J Biotechnol, 5 (11), 1142-45. doi:10.1055/s-2007-987042.
Renugadevi J, Milton prabu S, 2010. Quercetin protects against oxidative stress-related renal dysfunction by cadmium in rats. Exp Toxicol Pathol, 62(5), 471-81. doi:10.1016/j. etp.2009.06.006.
Satarug S, Baker JR, Urbenjapol S, Haswell-elkins M, Reilly PEB, Williams DJ, Moore MR, 2003. A global perspective on cadmium pollution and toxicity in non-occupationally exposed population. Toxicol Lett, 137(1-2), 65-83. doi:10.1016/S0378-4274(02)00381-8.
Schwartz GG, Reis IM, 2000. Is cadmium a cause of human pancreatic cancer? Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 9(2), 139-45.
Smith CJ, Livingston SD, Doolittle DJ, 1997. An international literature survey of “IARC group I carcinogens” reported in mainstream cigarette smoke. Food Chem Toxicol, 35(10- 11), 1107-30. doi:10.1016/S0278-6915(97)00063-X.
Wang L, Lin SQ, He YL, Liu G, Wang ZY, 2013. Protective effects of quercetin on cadmium-induced cytotoxicity in primary cultures of rat proximal tubular cells. Biomed Environ Sci, 26(4), 258-67. doi:10.3967/0895-3988.2013.04.004.
World Health Organization, 2010. Exposure to cadmium: a major public health concern, WHO 3-6. http://www.who. int/ipcs/features/cadmium.pdf.
Zargar S, Siddiqi NJ, Khalaf S, Daihan A, Wani TA, 2015. Protective effects of quercetin on cadmium fluoride induced oxidative stress at different intervals of time in mouse liver. Acta Biochim Pol, 62(2), 207-13.
Zohouri A, Tekeli SK, 1999. Radarda Kadmiyumun Metabolizma Üzerindeki Etkilerinin Araştırılması. İstanbul Üniv Vet Fak Derg, 25(2), 261-71.