Mehmet ELMALI, Hayriye Yeşim CAN, Yaşar ERGÜN²

Kronik mastitli inek sütlerinde metisiline dirençli Staphylococcus aureus

Amaç: Bu çalışmanın amaçları: i) sürekli tekrarlayan mastitli inek sütlerinden koagulaz pozitif stafilokok ve koagulaz negatif stafilokokları izole etmek, ii) izolatları Staphylococcus spp., Staphylococcus aureus ve metisiline dirençli Staphylococcus aureus yönünden multipleks PCR yöntemi ile doğrulamak ve iii) disk difüzyon yöntemi ile izolatların antimikrobiyel duyarlılığını belirlemektir.Gereç ve Yöntem: Bu kapsamda, Hatay ilinde sürekli tekrarlayan klinik mastit sorunu yaşayan süt ineklerinden toplam 52 adet süt örneği alındı. Koagulaz pozitif stafilokok ve koagulaz negatif stafilokokların izolasyon ve identifikasyonu klasik kültür tekniği ile yapıldı. Koagulaz pozitif stafilokok ve koagulaz negatif stafilokok olarak saptanan izolatların hepsi 16S rRNA, nuc ve mecA gen sekansları baz alınarak, sırasıyla Staphylococcus spp., S. aureus ve metisiline dirençli S. aureus yönünden multipleks PCR yöntemi ile doğrulandı. Ayrıca izolatların antimikrobiyel duyarlılığı araştırıldı.Bulgular: Süt örneklerinin % 48.0'inde pozitif sonuçlar elde edildi. Pozitif örneklerden toplam 56 adet stafilokok izolatı (22 adet koagulaz pozitif stafilokok ve 34 adet koagulaz negatif stafilokok) elde edildi. Koagulaz pozitif stafilokok izolatlarının 20'si (% 90.9) S. aureus olarak doğrulandı. S. aureus olarak doğrulanan izolatlarının 18'inde (% 90) mecA geni tespit edildi. Ayrıca, bu çalışmada izolatlarda disk difüzyon yöntemiyle, eritromisin (% 66), tetrasiklin (% 62.5), ampisilin (% 60.7), penisilin (% 60.7), sefoksitin (% 50), oksasilin (% 46.4) ve kloramfenikole (% 5.3) karşı direnç belirlendi Öneri: Çalışmada, kronik tekrarlayan mastitli inek sütlerinde antibiyotiklere dirençli stafilokokların ve özellikle metisiline dirençli S. aureus'un saptanması, bu bakterilerin süt ile birlikte gıda zinciri boyunca yayılma olasılığı açısından dikkate alınmalıdır.

Methicillin-resistant Staphylococcus aureus in milk from dairy cows with chronic mastitis

Aim: The aims of this study were: i) to isolate coagulase positive Staphylococcus (CPS) and coagulase-negative Staphylococcus (CNS) in milk from dairy cows with chronic recurrent mastitis, ii) to verify isolates in terms of Staphylococcus spp., Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) by multiplex PCR, and iii) to determine the antimicrobial susceptibility of the isolates by disk diffusion method. Materials and Methods: A total of 52 milk samples were collected from the dairy cows with chronic recurrent mastitis in Hatay province. Isolation and identification of CPS and CNS were detected by using classical culture method. All CPS and CNS isolates were investigated for antimicrobial susceptibility and also, were confirmed as Staphylococcus spp., S. aureus and MRSA by multiplex PCR based on 16S rRNA, nuc, and mecA gene sequences, respectively. Results: Positive results were found in 48.0% of the milk samp-les. A total of 56 Staphylococcus spp. isolates (22 CPS and 34 CNS) were obtained from the positive samples. Twenty (90.9%) of the CPS isolates were detected as S. aureus. The mecA gene was detected in 18 (90%) of the S. aureus isolates. By disk dif-fusion method, resistance to erythromycin (66%), tetracycli-ne (62.5%), ampicillin (60.7%), penicillin (60.7%), cefoxitin (50%), oxacillin (46.4%), and chloramphenicol (5.3%) was also determined in this study.Conclusion: In the study, the detection of antibiotic resistant Staphylococcus strains and especially MRSA in milk from dairy cows with chronic recurrent mastitis should be taken into account for potential spread through the dairy food chain

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