İstanbul'da paketsiz satılan evcil kuş yemlerinin besin içeriklerinin, mikrobiyolojik kontaminasyonun ve aflatoksin varlığının analizi
Amaç: Bu çalışmada İstanbul'da satılan paketsiz evcil kuş yemlerinin besleyici yeterliliği, mikrobiyolojik kalitesi ve aflatoksin içeriğinin tespit edilmesi amaçlanmıştır. Gereç ve Yöntem: İstanbul'un farklı bölgelerinden toplam 102 adet paketsiz yem örneği (35 kanarya, 37 muhabbet kuşu, 30 papağan) toplanmıştır. Tohum karışımlarından oluşan tüm örneklerin besin madde içeriği, mikrobiyolojik kalitesi ve aflatoksin içeriği analiz edilmiştir. Bulgular: Muhabbet kuşu yemlerinin ham protein (HP), ham yağ (HY) ve metabolize olabilir enerji (ME) içeriği ihtiyacı karşı- layabilecek düzeydedir. Ancak kanarya ve papağan yem örneklerinin HP, HY ve ME düzeyinin ihtiyaçtan daha yüksek düzeydedir. Kanarya ve muhabbet kuşu yemlerinin yaklaşık %40'ında (sırasıyla 3.19 ± 0.16 ve 3.25 ± 0.11 log cfu/g) , papağan yemlerinin ise tamamında (3.43±0.58 log cfu/g) koliform bakteri izole edilmiştir. Yem örneklerinin tamamında 3-5.5 log cfu/g oranında küf tespit edilmiştir. Salmonella izole edilememiştir. Aflatoksin B1, B2, G1 ve G2 hiçbir yem örneğinde bulunmamıştır. Öneri: Mikrobiyolojik sonuçlar evcil kuşlar üzerinde tehtid oluşturmazken, kanarya ve papağan yemlerinin dengesiz besin madde içeriğinin evcil kuşların sağlıklarını olumsuz etkileyebilecek düzeyde olduğu anlaşılmıştır.
Analysis of nutritional contents, microbiological contamination and presence of aflatoxins in unpacked feeds sold for pet-birds in Istanbul
Aim: In this study, it was aim to determine the nutritional efficiency, microbiological quality and aflatoxin contents of unpacked pet-bird feeds sold in Istanbul.Materials and Methods: A total of 102 unpacked feed samples sold for canary (n=35), budgerigar (n=37) and parrot (n=30) were collected from different districts in Istanbul. All the samples, consist of seed mixtures, were analysed regard to their nutritional content, microbiological quality and presence of aflatoxins. Results: Crude protein (CP), crude fat (CF) and metabolizable energy (ME) levels of the budgerigar feeds were met the nutritional requirements. However, nutritional contents (CP, CF, ME) of the canary and parrot feeds were higher than their requirements. In the microbiological analysis, Coliform group of bacteria were isolated from ~40% of budgerigar and canary feeds (3.19 ± 0.16 and 3.25 ± 0.11 log cfu/g, respectively), while isolated from all of the parrot feeds (100%; mean 3.43±0.58 log cfu/g). None of the 102 feeds were contaminated with Salmonella. Mould content of the feeds (100%) was ranging from 3 to 5.5 log cfu/g. Aflatoxin content (B1, B2, G1, G2) was not detected in feed samples.Conclusion: Regarding to the microbiological results of seed mixtures no threat is found for pet birds. However, it is assumed that nutritional imbalance formulation of canary and parrot feeds may affect adversely the pet birds’ health
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- Mamun MA, Bhuiyan MJU, Zinnah MA, Khair A, Hassan MM, Uddin MB. Seroprevalence and pathological investigation of salmonellosis in poultry. Eurasian J Vet Sci, 2011, 27, 4, 227-229.
- Aquino S, Corrêa B, 2011. Aflatoxins in Pet Foods. Torres-Pacheco I. A Risk to Special Consumers, Aflatoxins - Detection, Measurement and Control, InTech.
- Association of Official Analytical Chemists (AOAC), 1990. Animal Feed, In: Official methods of analysis, Ed; The Association of Official Analytical Chemists Inc, 15th ed, Virginia, USA, pp; 69-88.
- Bauck L, 1995. Nutritional problems in pet birds. Semin Avian Exot Pet, 4, 3-8.
- Bavelaar FJ, Beynen AC, 2004. Atherosclerosis in parrots: a review. Vet Quart ,26, 50-60.
- Bilal T, Aksakal DH, Sünnetci S, Keser O, Eseceli H, 2014. Detection of Aflatoxin, Zearalenone and Deoxynivalenol in Some Feed and Feedstuffs in Turkey. Pak Vet J, 34, 459-463.
- Boermans HJ, Leung MCK, 2007. Mycotoxins and the pet food industry: toxicological evidence and risk assessment. Int J Food Microbiol, 119, 95–102.
- Brightsmith DJ, 2012. Nutritional levels of diets fed to captive Amazon parrots: does mixing seed, produce, and pellets provide a healthy diet?. J Avian Med Surg, 26, 149-160.
- Brue RN, 1994. Nutrition, In: Avian Medicine: Principles and Application, Ed; Ritchie BW, Hsarrison GJ, Zantop D, Harrison LR, Wingers Publishing, Inc., Lake Worth, Florida, pp; 63-95.
- Copetti MV, Santurio JM, Cavalheiro AS, Alves SH, Ferreiro L, 2009. Comparison of different culture media for mycological evaluation of commercial pet food. Acta Sci Vet, 37, 329-335.
- FAO 2004. Assessing quality and safety of animal feeds. Animal production and health paper. No. 160. Food and Agriculture Organization of the United Nations, Rome.
- Harper EJ, Turner CL, 2000. Nutrition and energetics of the canary (Serinus canarius). Comp Biochem Phys B, 126, 271-281.
- Koutsos EA, Matson KD, Klasing KC, 2001a. Nutrition of birds in the order Psittaciformes: a review. J Avian Med Surg, 15, 257-275.
- Koutsos EA, Smith J, Woods LW, Klasing KC, 2001b. Adult cockatiells (Nymphicus hollandicus) metabolically adapt to high protein diets. J Nutr, 131, 2014-2020.
- Li SJ, Ediage EN, Saeger SD, Waeyenberghe LV, Garmyn A, Verlinden M, Ducatelle R, Croubels S, Haesebrouck F, Pasmans F, Martel A, 2013. Occurrence and pathology of mycotoxins in commercial parrot feeds. World Mycotoxin J, 6, 449-453.
- Lightfoot TL, Yeager JM, 2008. Pet bird toxicity and related environmental concerns. Vet Clin Exot Anim, 11, 229-259.
- Maciorowski KG, Herrera P, Jones FT, Pillai SD, Ricke SC, 2007. Effects on poultry and livestock of feed contamination with bacteria and fungi. Anim Feed Sci Tech, 133, 109-136.
- Martins ML, Martins HM, Bernardo F, 2003. Fungal flora and mycotoxins detection in commercial pet food. Rev Port Cienc Vet Revista, 98, 179-183.
- Official Journal (Resmi Gazete), 2014. Rescript on undesirable substances (contaminants) in feedstuffs (Yemlerde istenmeyen maddeler hakkında tebliğ), No. 2014/11/28977.
- Omurtag I, Smulders FJM, Hilbert F, Paulsen P, 2012. Microbiological condition of chicken doner sold in Vienna, Austria. Arch Lebensmittelhyg, 63, 142-146,.
- Orosz SE, 2014. Clinical avian nutrition. Vet Clin Exot Anim, 17, 397–413.
- Oruç H, Sonal S, Ceylan S, 2001. Total Aflatoxin, Nitrate and Nitrite in Bird Foods. J Fac Vet Med, 20, 35-38.
- Oruç HH, Sorucu A, Turkmen II, Arslan E, 2012. Determination of various mycotoxin concentrations in the feedstuffs and feed produced by a feed manufacturer in Turkey. Kafkas Univ J Fac Vet Med, 18, 633-638.
- Scudamore KA, Hetmanski MT, Nawaz S, Naylor J, Rainbird S, 1997. Determination of mycotoxins in pet foods sold for domestic pets and wild birds using linked-column immunoassay clean-up and HPLC. Food Addit Contam, 14, 175- 186.
- Scussel VM, Giordano BNE, Simão V, Rocha MW, dos Reis LFC, Xavier JJM, 2006. Mycotoxin evaluation in feed for pets using tandem liquid chromatography mass/mass. In Proceedings of the 9th International Working Conference on Stored Product Protection. Brazil, 6307.
- Ullrey DE, Allen ME, Baer DJ, 1991. Formulated diets versus seed mixtures for psittacines. J Nutr, 121, 193-205.
- Werquin GJDL, De Cock KJS, Ghysels PGC, 2005. Comparison of the nutrient analysis and caloric density of 30 commercial seed mixtures (in toto and dehulled) with 27 commercial diets for parrots. J Anim Physiol An N, 89, 215–221.