Tamer ÇAĞLAYAN², Erdoğan ŞEKER, Emrah SUR, Nariste KADIRALİEVA

Dağ nanesinin (Mentha caucasica) Japon bıldırcınlarının (Coturnix coturnix japonica) ince bağırsak histolojisi üzerindeki etkilerinin belirlenmesi

Amaç: Bu çalışma dağ nanesinin (Mentha caucasica) Japon bıldırcınlarının ince bağırsakları üzerindeki etkisinin ışık mikroskobik seviyede belirlenmesi amacıyla yapıldı. Gereç ve Yöntem: Çalışmada 1günlük 30 adet Japon bıldırcını kullanıldı. Bıldırcınlar, her biri 10 adet olmak üzere üç gruba ayrıldı. Hayvanlar 6 hafta boyunca kontrol, N1 (%0,75 oranında nane) ve N2 (%1,5 oranında nane) rasyonları ile beslendiler. Çalışma sonunda servikal dislokasyonla öldürülen hayvanlardan duodenum, jejunum ve ileum doku örnekleri alındı. Rutin histolojik işlemlerden sonra alınan seri kesitler Crossmon'un üçlü boyaması ve Periyodik Asit Schiff (PAS) reaksiyonu ile boyandılar. Tüm seri kesitler ışık mikroskobu ile değerlendirildikten sonra dijital kamera ile fotoğraflar çekildi ve kaydedildi. Villus yüksekliği, villus genişliği ve tunika muskularis kalınlıkları ölçüldü ve kadeh hücreleri sayıldı.Bulgular: Nane oranlarına bağlı olarak nane ile beslenen gruplardaki hayvanların duodenum, jejunum ve ileum dokularında vilusların yüksekliğinde, kript derinliğinde ve tunika muskularis kalınlığında artışlar tespit edilirken (p

Determination of the effects of Mentha caucasica on histology of small intestine in Japanese quail (Coturnix coturnix japonica)

Aim: This study was carried out to determine the effect of Mentha caucasica on histo-morphology of the small intestine at light microscopic level in Japanese quails. Materials and Methods: One day-old Japaneıse quail chicks (n=30) were used in the study. The quails were randomly divided into 3 groups which consist of 10 animals for each.as control, M1 (0,75% mint content) and M2 (1,5% mint content). Animals were fed diets containing different mint content for six weeks. At the end of the study, the birds were killed by cervical dislocation. The duodenum, jejunum and ileum samples were collected. After routine hitological process, the serial sections were stained with Crossmon's trichrome and Periodic Acid Schiff (PAS) reaction. All sections were evaluated under the light microscope and were photographed by digital camera and were recorded. Villus height, villus width, crypt depth, and thickness of tunica muscularis were measured and Goblet cell numbers were calculated.Results: The average villus height, crypt depth, and thickness of tunica muscularis were increased in the duodenum, jejunum, and ileum depending on the mint content (p

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