Konya’da tüketilen tavuk eti ve iç organlarında Salmonella spp. varlığı ve antibiyotik direnci

Amaç: Bu çalışmada tüketime sunulan tavuk eti ve sakatatlarda Salmonella spp. ve iki önemli Salmonella serotipinin (S. Thyphimurium ve S. Enteritidis) varlığı ve izolatların antimikrobiyal direncinin belirlenmesi amaçlandı. Gereç ve Yöntem: Araştırmada Konya ilindeki süpermarket ve kasaplarda tüketime sunulan tavukların karaciğer (n = 40), taşlık (n = 40), kalp (n = 30), deri (n = 30), baget (n = 10) ve kanat (n = 20) örnekleri klasik kültür tekniği ile analiz edildi. Şüpheli Salmonella spp. izolatlarının moleküler düzeyde doğrulanması amacıyla gerçekleştirilen PCR uygulamasında Inv-A gen bölgesine ait primerler kullanıldı. S. Thyphimurium ve S. Enteritidis’e ait gen bölgelerinin tespitinde ise Flic-C ve IE-1 primerleri ile dupleks PCR (d-PCR) uygulandı. Bulgular: Toplam 170 örneğin 43'ü (% 25.29) Salmonella spp. pozitif olarak tespit edildi. d-PCR sonuçlarına göre izolatların hiçbirinde S. Thyphimurium ya da S. Enteritidis saptanmadı. İzolatlarda klindamisin, oksasilin, teikoplanin (% 100), vankomisin (% 79.1), eritromisin (% 79.1), nalidiksik asit (% 65.1), penisilin G (% 60.5) sefalotin (% 48,8), sülfametoksazol-trimetoprim ( % 37.2), tetrasiklin (% 37.2), ampisilin (% 23.3), kanamisin (% 18.6), kloramfenikol (% 11.6) amikasin, sefazolin, siprofloksasin ve gentamisine (% 4.7) direnç saptandı. Tüm izolatlar amoksisilin / klavulanik asit ve sefixime duyarlı bulundu. Öneri: Araştırmada S. Enteritidis ve S. Typhimurium'un tespit edilmemiş olması halk sağlığı açısından olumlu kabul edilmiştir. Bunun yanısıra, yasal mevzuat açısından daha düşük insidense sahip patojen türlerin tespit edilmesine yönelik çalışmalara dikkat çekmenin önemli olduğu düşünülmektedir. Ayrıca sık rastlanan patojen türlerin tespit edilmemesine rağmen, tür düzeyinde tespit edilen izolatlardaki antibiyotik direnç sonuçları antibiyotik sörvelans veritabanı için önemli bulunmuştur.

Presence and antibiotic resistance of Salmonella spp. isolated from chicken meat and giblets consumed in Konya, Turkey

Aim: The present study was on the detection of Salmonella spp. and twoimportant Salmonella serotypes (S. Thyphimurium and S. Enteritidis) inchicken meat and giblets and also determination of antimicrobial resistanceof the isolates.Materials and Methods: In this study, livers (n=40), gizzards (n=40),hearts (n=30), skins (n=30), drumsticks (n=10) and wings (n=20) werecollected from supermarkets and butcher shops in Konya, Turkey. Thesamples were analyzed by Classical Cultural Technique. Molecularconfirmation of the suspicious colonies was carried out using Inv-A gene-based PCR. Flic-C and IE-1 primers were used by duplex PCR for S.Thyphimurium and S. Enteritidis respectively. Antibiotic resistance ofthe isolates was determined by the disk diffusion method.Results: Forty-three (25.29 %) of 170 samples were positive forSalmonella spp. According to the d-PCR assay, neither S.Thyphimurium nor S. Enteritidis was not detected. The resistance toclindamycin, oxacillin, teicoplanin were evident 100 % and resistanceto vancomycin (79.1 %), erythromycin (79.1 %), nalidixic acid (65.1%), penicillin G (60.5 %) cephalothin (48.8 %), sulfamethoxazoletrimethoprim(37.2 %), tetracycline (37.2 %), ampicillin (23.3 %),kanamycin (18.6 %), chloramphenicol (11.6 %) amikacin, cephazoline,ciprofloxacin, gentamycin (4.7 %) was also detected. All isolates weresusceptible to amoxicillin/clavulanic acid and cefixime.Conclusion: The results indicated that S. Enteritidis and S. Typhimuriumwere not identified and it was considered satisfactory in terms ofpublic health. It should be still important to note the studies to identifyspecies with lower pathogenic incidences for legal legislation. Furthermore,even the most common pathogenic species cannot be detected,the results of antibiotic resistance in isolates were noteworthy for antibioticsurveillance database.

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