Kızıl şahinlerden izole edilen Escherichia coli suşlarının antimikrobiyal direnç profilleri
Amaç: Bu çalışmada, kızıl şahinlerden (Buteo rufinus) toplanan kloakal sıvap örneklerinin bakteriyolojik muayenesi ve izole edilen Escherichia coli (E. coli) suşlarının antibakteriyel dirençlerinin belirlenmesi amaçlandı. Gereç ve Yöntem: Çeşitli şikâyetlerle Afyon Kocatepe Üniversitesi Veteriner Fakültesi kliniklerine getirilen kızıl şahinlerden 24 kloakal sıvap örneği toplandı. Örnekler besi yerlerine ekilerek 37 °C de aerobik ve mikroaerofilik olarak inkübe edildi. Salmonella spp. varlığı ISO 6579 protokolüne göre yapıldı. Daha sonra izole edilen bakterilerin antibiyotik duyarlılıkları belirlendi. Bulgular: Örneklerden toplam 24 E. coli suşu izole edildi. Ayrıca, bir Enterococcus faecalis izolatı da izole edildi. E. coli izolatlarının tamamı florfenikol ve siprofoksasine karşı duyarlı olarak bulunurken diğer antibiyotiklere karşı değişen oranlarda dirençlilik tespit edildi. Öneri: Bu çalışma ile ülkemizdeki kızıl şahinlerden elde edilen kloakal sıvapların bakteriyolojik muayenesi ilk kez yapıldı. Ayrıca, antibiyotik kullanılmayan kızıl şahinlerden izole edilen E. coli izolatlarının farklı antibiyotiklere değişen oranlarda dirençli olması da anlamlı bulundu.
Antimicrobial resistances of Escherichia coli isolated from Buteo rufinus
Aim: In this study, it was aimed to determine microbiologicalexamination of cloacal swab specimens taken from Long-leggedBuzzards (Buteo rufinus) and antibiotic resistance of isolatedEscherichia coli (E. coli) strains.Materials and Methods: The cloacal swab specimens wereobtained from 24 Long-legged Buzzards which were admittedto Afyon Kocatepe University Veterinary Faculty clinics. Thesamples were cultured on different media for various bacteria(Salmonella species, E. coli, Mycoplasma, Gram-positive cocci,etc.). The media were incubated at 37°C in aerobic and microaerophilicconditions. ISO 6579 protocol was applied for Salmonella.In addition, antibiotic susceptibilities of the isolatedbacteria were determined.Results: Twenty-four E. coli were isolated from all cloacal swabsamples. In addition, Enterococcus faecalis was isolated fromone sample. While all E. coli isolates were susceptible to florfenicoland ciprofloxacin, other antibiotics were found to be susceptibleat different rates.Conclusion: This study is important because it is the first microbiologicalexamination of cloacal swaps of Long-legged Buzzardsin this Country. In addition, it was significantly evaluatedthat E. coli strains were resistant to various antibiotics with differentratios while no antibiotics are used using antibiotics inLong-legged Buzzards.
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