Keskin E, Uluışık D. Ratlarda deneysel olarak oluşturulan diyabette CoQ10 uygulamasının karaciğer enzimleri üzerine etkilerine yönelik çalışma

.Amaç: Bu çalışmada deneysel olarak oluşturulan diyabette CoQ10 uygulamasının bazı karaciğer enzimleri üzerine etkilerinin belirlenmesi amaçlanmıştır.Gereç ve Yöntem: Çalışmada bu amaçla 38 adet sağlıklı erkek Wistar Albino rat kullanıldı. Hayvanlar 5 gruba ayrıldı. Çalışmada dört hafta boyunca, grup 1 standart rat yemi ile beslenirken, grup 2'deki hayvanlara bu yeme ilaveten intraperitoneal (IP) olarak 0,3 ml mısır yağı ve grup 3'deki hayvanlara aynı yeme ilaveten IP olarak günde 10 mg/kg CoQ10 uygulaması yapıldı. Grup 4'deki hayvanlarda 40 mg/kg dozunda 0.1 M sitrat buffer (pH 4.5) içerisinde streptozotosinin günde tek doz olmak üzere iki kere subkutan (SC) enjeksiyonuyla deneysel diyabet oluşturuldu. Grup 5'deki hayvanlarda 40 mg/kg dozunda 0.1 M sitrat buffer (pH 4.5) içerisinde streptozotosinin günde tek doz olmak üzere iki kere SC enjeksiyonuyla deneysel diyabet oluşturuldu ve sonra dört hafta boyunca IP olarak günde 10 mg/kg CoQ10 uygulaması yapıldı. Dördüncü haftanın sonunda bütün hayvanlardan kan örnekleri alındı. Bu plazma örneklerinde, aspartat aminotransferaz (AST), alanin aminotransferaz (ALT), alkalin fosfataz (ALP) ve ?-glutamil transpeptidaz (GGT) düzeyleri belirlendi.Bulgular: Çalışmada diyabet oluşturulan grupta AST, ALT, ALP ve GGT düzeyleri kontrol grubuna göre önemli artışlar (P

A Study on Coenzyme Q10, experimentally induced diabetes and liver enzymes in rats

Keskin E, Uluisik D. A Study on Coenzyme Q10, Experimentally In-duced Diabetes and Liver Enzymes in Rats.Aim: This study was conducted to determine the effects of CoQ10 supplementation on some liver enzymes in diabetes status. Materials and Methods: In the study, 38 healthy male Wistar Albino rats were used. The rats were divided into five groups. The animals in group 1, group 2 and group 3 were received standard diet, standard diet plus IP 0,3 ml corn oil and standard diet plus IP 10 mg/kg CoQ10 for four weeks, respectively. In group 4, diabetes was performed via SC injections of Streptozotocin (STZ) at dose of 40 mg/kg in 0.1 M citrate buffer (pH 4.5) single daily dose for two days. In group 5, diabetes was made via SC injections of STZ at a dose of 40 mg/kg in 0.1 M citrate buffer (pH 4.5) single daily dose for two days and then received IP 10 mg/kg CoQ10 for four weeks. At the end of the fourth week, blood samples were taken from all animals. In plasma samples, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and γ-glutamyl transpeptidase (GGT) levels were determined.Results: In this study, AST, ALT, ALP and GGT levels in diabetic group significantly increased compared with control group levels (P

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