Erdoğan UZLU³, Volkan YILMAZ, Yakup YILDIRIM, Fethiye ÇÖVEN², Ayşegül ÇOBAN, Çağan Hakkı ŞEKERCİOĞLU, Emrah ÇOBAN

Investigation of West Nile virus infection in brown bears (ursus arctos) in Turkey

.Amaç: Bu çalışmada, Türkiye'de Sarıkamış/Kars yöresindeki doğal parkta, serbest dolaşımda bulunan boz ayılarda Batı Nil virüsünün (BNV) varlığı/oranı serolojik ve virolojik olarak araştırıldı.Gereç ve Yöntem: Bu amaçla 11 adet boz ayıdan alınan kan örneklerinde BNV spesifik antikor varlığı commercial competitive enzyme-linked immunosorbent assay (C-ELISA) yöntemi ile tarandı. Toplanan kan örneklerinden elde lökosit örneklerinde BNV nükleik asidinin belirlenmesi amacıyla da Reverse Transkripsiyon-Polimeraz Zincir Reaksiyonu (RTPZR) tekniği kullanıldı.Bulgular: Örneklerin hiçbirinde BNV nükleik asidi ve BNV spesifik antikoru tespit edilmedi.Öneri: Türkiye'de serbest dolaşımdaki vahşi memeli hayvanlar ve boz ayılarda varlığı/oranı bilinmeyen BNV enfeksiyonunun ileriki dönemlerde daha ayrıntılı araştırılması gerektiği düşünülmektedir. Bu araştırma Türkiye'de serbest dolaşımdaki boz ayılarda BNV enfeksiyonu ile ilgili yapılan ilk çalışmadır.

Yıldırım Y, Çöven F, Uzlu E, Yılmaz V, Çoban E, Çoban A, Şekercioğlu ÇH. Türkiye’deki boz ayılarda Batı Nil Virüsü’nün araştırılması

Aim: This study is a serological and virological examination of the West Nile Virus (WNV) in free-ranging brown bears (Ursus arctos) in Sarikamis district of Kars province of Turkey. Materials and Methods: For this purpose, blood samples from 11 brown bears were tested for antibodies against WNV using a commercial competitive enzyme-linked immunosorbent assay (C-ELISA). Reverse transcription polymerase chain reaction (RT-PCR) technique was used to determine the presence of West Nile Virus nucleic acid. Results: Seropositivity rate was detected to be zero % and West Nile virus nucleic acid was not found in samples. Conclusions: The significance of WNV infection in brown bears and other free-ranging mammals in the Turkey is unknown at this time and will require further investigation. This is the first report of WNV infection in brown bears in Turkey

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