Hasan Hüseyin Hadimli, Aslı Sakmanoğlu, Gökçenur Sanioğlu Gölen

Farelerde Salmonella enfeksiyonlarına karşı bakteriyofaj tedavisinin etkinliğinin belirlenmesi

Amaç: Antibiyotiğe dirençli salmonella kaynaklı enfeksiyonlarının artışı dünya genelinde önemli sorunlara neden olmaktadır. Bu çalışmada, alternatif uygulama olarak sığır dışkılarından izole edilen dört ΦSP – 3 litik faj (Salmonella Dublin, S. Typhimurium, S. Anatum, S. Kentucky) ile kokteyl hazırlanarak fare modellerinde bakteriyofaj tedavisinin etkinliğinin araştırılması amaçlandı. Gereç ve Yöntem: Bakteriyofaj tedavi için 4 farklı salmonella bakteriyofaj izolatından kokteyl hazırlandı. Toplam 80 fare (toplam 8 deneme grubu ve her grupta 10 fare) salmonella türleri ile oral yolla çelınç yapıldı. Çelınç sonrası, farelere oral yolla bakteriyofaj kokteyli verildi. Fareler 20 gün boyunca hastalık oluşumu ve ölüm yönünden gözlendi. Aynı zamanda, dışkı ile salmonella türlerinin saçılımı üzerine bakteriyofaj tedavinin etkisini belirlemek için dışkı örnekleri bakteriyolojik olarak incelendi. Bulgular: S. Dublin ve S. Typhimurium ile çelınç yapılan 2’şer farede hastalık ve ölüm gözlendi. Ayrıca, sadece ölen 2’şer farenin iç organlarından S. Dublin ve S. Typhimurium izolatlarının geri izolasyonu yapıldı. S. Kentucky ve S. Anatum antijenleri ile çelınç yapılan farelerde hastalık ve ölüm vakası gözlenmedi ve farelerin iç organlarından salmonella izolasyonu yapılmadı. Bununla birlikte, tüm gruplardaki farelerin dışkı örneklerinden salmonella türlerinin geri izolasyonları yapıldı. Öneri: Sığırların dışkısından elde edilen bakteriyofaj kokteyllerinin farelerde mortalite ve morbiditeyi engellediği ve dışkı yoluyla Salmonella spp. saçılımını azalttığı belirlendi. Bu sebeple salmonella enfeksiyonlarına karşı korunmada bakteriyofaj terapinin kullanılabileceği kanaatine varıldı.

Evaluation of the effectiveness of bacteriophage therapy against Salmonella infections in mice

Aim: The increase of infections caused by antimicrobial resistant salmonellae has become a serious problem worldwide. In this study, it was aimed to investigate the efficacy of bacteriophage treatment in mouse models by preparing a cocktail with four ΦSP-3 lytic phages (Salmonella Dublin, S. Typhimurium, S. Anatum, S. Kentucky) isolated from cattle feces as an alternative application. Materials and Methods: A total of 80 mice (total 8 experimental groups and each group included 10 mice) were challenged with salmonella strains by oral route. After challenge, bacteriohage coctail to mice were administrated by oral route. Mice were observed for occurence of morbidity and mortality for 20 days. Also, faecal samples were bacteriologically examined to determine the effect of bacteriophage treatment on the spreading of Salmonella species with feces. Results: The morbidity and mortality were observed in two mice, administered bacteriophage coctail following challenge with S. Dublin and S. Typhimurium. In addition, re-isolation of S. Dublin and S. Typhimurium from internal organs in 2 death mice were done. The morbidity and mortality in mice challenged with S. Kentucky and S. Anatum and administered bacteriophage coctail was not observed and re-isolation from internal organs were not carried out. However, re-isolation from feces of mice in all groups were made. Conclusion: The findings of present study revealed that bacteriophage cocktails obtained from cattle faeces prevented mortality and morbidity in Salmonella infected mice, and reduced the spread of Salmonella spp. Therefore, bacteriophage therapy could be used for protection against salmonella infections.

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