Israa Mahmoud Almahad, Mehmet Borga Tırpan

Depolamada sıvı nitrojene bir alternatif olarak -152°C ultra dondurucuların kullanımının araştırılması

Amaç: Bu çalışmanın amacı, dondurulmuş spermanın -152 °C derin dondurucuda sıvı azot içinde depolanmasının geleneksel yöntemlerde kullanılan depolama yöntemine alternatif bir çözüm olup olmayacağının araştırılmasıdır. Gereç ve Yöntem: Farklı günlerde iki boğadan toplam 80 payet toplandı. Aynı günde toplanan spermalar kontrol ve deney grubu olmak üzere iki farklı gruba ayrıldı. Çalışmanın deney grubunu bir hafta boyunca -152 °C'de saklanan spermalar oluştururken, kontrol grubunu ise geleneksel şekilde -196 °C'de saklanmış spermalar oluşturdu Deney süresinin sonunda donmuş spermalar, 30 saniye boyunca 37 °C su banyosunda çözdürüldü. Sonra CASA sistemi kullanılarak spermanın hareket parametreleri ve morfolojik özelikleri incelendi. Bulgular: CASA sistemi kullanılarak yapılan değerlendirmenin sonucunda, -196 °C'de dondurulmuş ve saklanmış spermanın hareketi ve ilerleme hareketi (%69.66, %37.61)-152 °C'de (% 54.54,% 28.72) saklanan değerlerden daha yüksek bulundu (p

Use of ultra-freezers of -152 °C as a viable alternative to liquid nitrogen in storing

Aim: This study aims to investigate the utilization of the ultra-freezer to store the semen at a -152 °C as an alternative method to traditional semen storage methods Materials and Methods: A total of 80 straws were collected from two bulls on different days. The semen collected on the same day were sorted into two different groups. The experimental group of the study was composed of sperm stored at -152 °C for one week, while the control group was composed of sperm stored at -196 °C using the traditional storage method. At the end of the experimental period, frozen semen at -196 °C and -152 °C were thawed at a temperature of 37 °C water bath for 30 seconds. Then, the spermatological parameters and morphological properties of the sperm were examined by using a computer-assisted sperm analysis system (CASA). Results: The evaluation of semen samples yielded the following results; the motility and progressive motility of the frozen and stored sperm (69.66%; 37.61%) at -196 °C were higher than the values (54.54%; 28.72%) stored at -152 °C (p

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