Çörek otu tohumu ve timokinonun broylerlerde aflatoksinin neden olduğu relatif karaciğer ağırlığı artışı üzerine koruyucu etkisi

Amaç: Bu çalışmada çörek otu tohumu ve bunun farmasötik olarak etkin maddesi timokinonun, broylerlerde aflatoksikozisde şekille- nen relatif karaciğer ağırlık artışını önleyici etkinliklerinin belirlen- mesi amaçlanmıştır. Gereç ve Yöntem: Çalışmada 120 adet 1 günlük broyler civciv kul- lanıldı. Bu civcivler 6 eşit gruba bölünerek 28 gün beslendi. Hayvan- lara, gruplarına göre (Kontrol, AF, NS, TQ, AF+NS ve AF+TQ) 2 mg/ kg total aflatoksin (AF; AFB1, AFB2, AFG1 ve AFG2), % 5 çörek otu tohumu (NS) ve 300 mg/kg timokinon (TQ) içeren yemler verildi. Deneme süresi sonunda gruplardaki 10’ar pilicin canlı ağırlıkları belirlendikten sonra dekapitasyon ile ötenazi uygulanarak sistemik nekropsileri yapıldı. Aflatoksin için hedef organ kabul edilen karaci- ğer uzaklaştırılarak relatiforgan ağırlığının belirlenmesi için tartıldı. Karaciğerin relatif organ ağırlığı % olarak (karaciğer ağırlığı x 100 / canlı vücut ağırlığı) hesaplandı. Bulgular: Aflatoksin uygulamasının relatif karaciğer ağırlığını önemli oranda artırdığı belirlendi (P

Protective effect of nigella sativa and thymoquinone on relative liver weight increasecaused by aflatoxin in broilers

Aim: It is aimed to determine the preventive effect of black seed (Ni-gella sativa L; NS) and its pharmaceutically active substance volatilefatty acid thymoquinone on relative liver weight increase in broilerin aflatoxicosis..Materials and Methods: One hundred twenty 1-d-old broiler chickswere used and divided into 6 equal groups and fed for 28 days. Ani-mals were received feed with 2 mg/kg total aflatoxin (AF; AFB1,AFB2, AFG1 and AFG2), 5% black seed (NS) and 300 mg/kg thymo-quinone (TQ), according to the groups; Control, AF, NS, TQ, AF+NSand AF+TQ. At the end of the experiment, euthanasia was performedby decapitation after determination of the live weight of ten broi-ler chickens in all groups, and systemic necropsies were performed.The liver, the main target organ for AF, was removed and weighedto determine relative organ weight. The relative liver weights werecalculated as % (liver weight x 100 / live body weight).Results: Aflatotoxin application significantly increased the relativeweight of the liver (P

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