Fatma GÜLER, Tolunay KOZLU, Yaşar ERGÜN, Beyza SUVARIKLI ALAN, Tolga TUTAR

Theranekron®'un sağlıklı ve iskemi-reperfüzyon hasarı oluşturulan rat ovaryumunda etkisi

Bu çalışmada sağlıklı ve iskemi-reperfüzyon yaralanmalı sıçan modelinde Theranekron®'un yumurtalıklar üzerindeki etkisi değerlendirildi. Otuz sekiz adet nulligravida dişi Wistar Albino rat aşağıdaki gibi gruplara ayrıldı; Grup 1: kontrol, Grup 2: Theranekron® (tek doz 0,3 mg/kg, intraperitoneal, 3 saat) Grup 3: iskemi-reperfüzyon ve Grup 4: iskemi-reperfüzyon+ Theranekron® (tek doz 0,3 mg/ kg, intraperitoneal, 3 saat). Her grup biyokimyasal ve histolojik olarak analiz edildi. Grup 1'e göre Grup 2'de atretik folikül ve interstisyel hücre sayısında anlamlı artış vardı. Ayrıca nitrit, nitrat ve malondialdehit (MDA) seviyeleri Grup 2'de anlamlı artış gösterdi. Grup 3'te yoğun hiperemi ve kanamalar mevcuttu. Grup 2'ye göre korteksi çevreleyen ve korpus luteumdaki foliküllerde ve atretik folikül sayısında belirgin artış oldu. Grup 3'teki nitrit ve nitrat seviyeleri Grup 2 ile benzer, ancak MDA seviyeleri Grup 2'den düşüktü. Grup 4, Grup 2 ve Grup 3 ile karşılaştırıldığında interstisyel hücrelerde çok hafif azalma gösterdi ve ayrıca daha az atretik folikül gözlendi. Bu grupta MDA düzeyleri Grup 2'ye göre azalmış, kontrol ve Grup 3'e göre yükselmiştir. Sonuçlara dayanarak, Theranekron®'un sağlam yumurtalıklar üzerinde bazı dejeneratif etkilere sahip olabileceğini ve yumurtalıklarda iskemi reperfüzyon hasarı üzerinde iyileştirici etkileri olabileceğini tahmin edebiliriz. Ancak Theranekron®'un sıçan yumurtalıklarında iskemi reperfüzyon hasarı üzerine optimum iyileştirici dozunu belirlemek için daha ileri çalışmalara ihtiyaç vardır.

Anahtar Kelimeler:

İskemi-reperfüzyon, Malondialdehit, Nitrat/Nitrit, Theranekron®, Rat

The effect of Theranekron® in intact and with ischemia-reperfusion injury rat ovary

In this study, the effect of Theranekron® on ovaries were evaluated in healthy and ischemia- reperfusion injury rat model. Thirty-eight female, nulligravida Wistar Albino rats were divided into groups as follows; Group 1: control, Group 2: Theranekron® (single dose of 0,3 mg/kg, intraperitoneal, for 3 hours) Group 3: ischemia-reperfusion and Group 4: ischemia- reperfusion+ Theranekron® (single dose 0,3 mg/kg, intraperitoneal, for 3 hours). Each groups were analyzed biochemically and histologically. As compared with Group 1, there was a significant increase in the number of atretic follicles and interstitial cells in Group 2. Also, nitrite, nitrate, and malondialdehyde (MDA) levels increased significantly in Group 2. Group 3 represented intense hyperemia and hemorrhages on follicles surrounding the cortex and in the corpus luteum and marked increase in the number of atretic follicles, compared to Group 2. Nitrite and nitrate levels in Group 3 were similar to those in Group 2, but MDA levels were lower than those in Group 2. Group 4 showed very slight decrease in interstitial cells compared with Group 2 and Group 3 and also fewer atretic follicles were observed. In this group, MDA levels decreased compared to Group 2 and increased compared to control and Group 3. Based on the results, we may speculate that Theranekron® may have some degenerative effects on intact ovaries, and may have ameliorationg effects on ischemia reperfusion injury in ovaries. But, further studies are needed to determine the optimum remedial dose of Theranekron® on ischemia reperfusion injury in rat ovaries.

Keywords:

Ischemia-reperfusion, Malondialdehyde, Nitrate/Nitrite, Rat, Theranekron®,

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