PPR (koyun keçi vebası) ve mavidil aşısının merinos ırkı koyunlarda eş zamanlı uygulanabilirliğinin araştırılması

Bulaşıcı hayvan hastalıklar arasında yer alan PPR ve Mavi dil, ciddi ekonomik kayıplara sebep olmalarından dolayı, Dünya Hayvan Sağlığı Örgütü (OIE) tarafından ihbarı zorunlu hastalıklar içinde yer almaktadır. PPR ve Mavi dil aşıları, her iki hastalığın birlikte görüldüğü ülkelerde yaygın olarak kullanılmaktadır. Bu çalışmada, PPR ve Mavi dil aşılarının aynı anda uygulanarak koyunlardaki antikor yanıtı üzerine etkilerinin incelenmesi amaçlanmıştır. Bu amaçla çalışmanın birinci basamağında, seçilen hayvanların aşılama öncesi kanları alınarak her iki hastalık yönünden araştırılmıştır. İkinci basamağında, tekli PPR aşısı grubu (Grup 1; genç kuzu n=10, yetişkin koyun n=10), tekli BT-4 aşısı grubu (Grup 2; genç kuzu n=10, yetişkin koyun n=10), PPR ve BT-4 ile eş zamanlı aşılı grup (Grup 3; genç kuzu n=10, yetişkin koyun n=10) ve aşısız kontrol grubu (Grup 4; genç kuzu n=5, yetişkin koyun n=5) olmak üzere 4 koyun grubu kullanılmıştır. Çalışmanın üçüncü basamağında, seçilen hayvanlardan nötralize antikorlar yönünden test edilmesi amacıyla 0. gün, 1., 3. ve 6. aylarda serum örnekleri toplanarak toplamda 280 adet serum numunesi PPR ve Mavi dil antikorları virus nötralizasyon testi ile nötralizan antikorlar yönünden kontrol edilmiştir. Gruplar arasında nötralizan antikor seviyeleri kıyaslandığında grup 3’ün 1. ve 2. gruplardan istatistiksel olarak anlamlı bir fark (p >0.05) göstermediği görülmüştür. Çalışma sonucunda, PPR ve Mavidil aşılarının eş zamanlı olarak uygulanabileceği, iş gücü ve ekonomik açıdan tasarruf sağlayacağı tespit edilmiştir.

Anahtar Kelimeler:

Aşı, BTV, PPRV, bağışıklık

INVESTIGATION OF SIMULTANEOUS ADMINISTRATION OF PPR (PESTE DES PETITS RUMINANTS) AND BLUETONGUE VACCINES IN MERINO SHEEP

Abstract: Peste des Petits Ruminants (PPR) and Bluetongue (BT), which are among the contagious animal diseases, cause serious economic losses and are included in the notifiable diseases by the World Organization for Animal Health (WOAH). PPR and BT vaccines are widely used in countries where outbreaks occur. In this study, we aimed to examine the neutralizing antibody response of simultaneously administered PPR and BT serotype 4 vaccines in sheep. For this purpose, a study was performed under field conditions in a state-owned breeding farm. All the experimental animals were confirmed seronegative by serum neutralization test (SNT) for antibodies to the PPR virus (PPRV) and BT virus serotype 4 (BTV4). Subsequently, the experimental animals were divided into four groups. Group 1 (Group 1/PPR; lamb n=10, sheep n=10) and group 2 (Group 2/BTV4; lamb n=10, sheep n=10) were vaccinated separately with the PPR and BTV4 vaccines. Group 3 was vaccinated simultaneously with PPR and BTV4 (Group 3/PPR-BTV4; lamb n=10, sheep n=10). Group 4 was unvaccinated and served as the control. In each of the experimental groups, the vaccinations were carried out only once. Post-vaccination blood samples were collected at intervals of 1, 3, and 6 months. A total of 280 post-vaccination blood serum samples were evaluated with SNT for PPRV and BTV4 neutralizing antibodies. When the neutralizing antibody levels between the groups were compared, group 3 did not show any statistically significant difference (p>0.05) from group 1 and group 2. Based on the antibody response of simultaneous vaccination, it is shown that the simultaneous vaccination could be administered on the field, and could be labor and cost-effective.

Keywords:

BTV, PPRV, immunity, simultaneous administration,

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