Esra DEMİRBAŞ, Cansu AKTAŞ, Volkan KAYDU, Enescan AKSOY, İlker HANCI, Ünver Oğuzhan TEKAY, Meltem ÖZER, Ertan Emek ONUK

Yersinia ruckeri İzolatlarında Biyofilm Oluşumunun ve Bazı Virulens Faktörlerinin Belirlenmesi

Çalışmada gökkuşağı alabalıklarından izole edilen 20 adet Yersinia ruckeri izolatının biyofilm oluşturma yeteneği ve bazı virülens faktörleri fenotipik olarak incelendi. İzolatların biyofilm oluşturma yeteneklerinin belirlenmesinde Kongo Red Agar (CRA), Modifiye Tüp Aderans (Christensen) ve Mikroplak yöntemleri kullanıldı. İzolatların hareket özelliği, hemolitik aktivitesi, lipaz aktivitesi ve proteaz aktivitesi fenotipik metotlar ile ortaya konuldu. CRA ve Christensen yöntemleri ile izolatlar biyofilm oluşturmazken mikroplak yönteminde izolatların büyük bir kısmının zayıf düzeyde biyofilm oluşturduğu saptandı. İzolatların 13’ünün hareketli ve lipaz aktivitesine sahip olduğu belirlenirken, 14 izolatın ise proteaz aktivitesine sahip olduğu belirlendi. Ek olarak izolatların tamamının nonhemolitik olduğu saptandı. Çalışma sonuçlarına göre sadece hareket ve lipaz aktivitesi arasında istatiksel olarak anlamlı, pozitif yönlü, güçlü bir korelasyon saptandı. Mikroorganizmaların patojenitesi virulens faktörleri ve biyofilm oluşumu ile bağlantılı bir süreçtir. Dolayısıyla Y. ruckeri izolatlarının virulens faktörlerinin ve biyofilm oluşumlarının periyodik olarak izlenmesi, Y. ruckeri enfeksiyonlarının önlenmesi veya tedavi edilmesinde anti-biyofilm terapilerinin geliştirilmesi gibi yeni stratejilerin oluşturulmasına önemli katkılar sağlayacaktır.

Anahtar Kelimeler:

Akuakültür, Biyofilm, Virulens faktörleri, Yersinia ruckeri

Determination of Biofilm Formation and Some Virulence Factors in Yersinia ruckeri Isolates

In the current study, biofilm forming ability and some virulence factors of 20 Yersinia ruckeri isolates obtained from rainbow trout were examined phenotypically. The Congo Red Agar (CRA), Modified Tube Aderans (Christensen) and Microplate methods were used to determine the biofilm forming ability of these isolates. The motility characteristics, hemolytic activity, lipase activity and protease activity of the isolates were determined by phenotypic tests. It was determined that the majority of the isolates produced a weak biofilm when examined using the microplate method, despite the fact that isolates did not produce biofilms when evaluated using the CRA and Christensen methods. While 14 isolates were discovered to have protease activity, 13 of the isolates were determined to be motile and to have lipase activity. All of the isolates were also determined to be nonhemolytic. According to the study's results, a statistically significant, positive and strong correlation was found only between motility and lipase activity. The pathogenicity of microorganisms is associated with virulence factors and biofilm formation. Therefore routine monitoring of the virulence factors and biofilm formation of Y. ruckeri isolates will significantly contribute to the establishment of novel techniques such as the development of anti-biofilm therapies for prevention or treatment of Y. ruckeri infections.

Keywords:

Yersinia ruckeri, Aquaculture, Biofilm, Virulence factors,

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