Nuray ŞAHİN, Murat YILDIRIM, Sibel KIZIL

Koyunlardan İzole Edilen Shiga Toksin Üreten Escherichia coli Suşlarının Virülens Faktörlerinin Araştırılması

Tüm dünyada, Shiga toksin üreten Escherichia coli (STEC), önemli zoonotik patojen olarak kabul edilmektedir. Başta sığırlar olmak üzere geviş getirenler ana rezervuarlarıdır, ancak küçükbaş hayvanların insan enfeksiyonlarının epidemiyolojisindeki rolü pek çok ülkede kapsamlı bir şekilde değerlendirilmemiştir. Bu çalışmada koyunlarda Escherichia coli suşları içerisinde Shiga toksin üreten izolatların Stx1, Stx2, eae ve ehxa genlerinin shiga toksin alt tiplerini belirlenmesi ve bunlara ırk, cinsiyeti gibi değişkenlerin karşılaştırmalı etkisini belirlemek amaçlanmıştır. Bu çalışmada, Ankara ili ve çevresindeki 12 farklı koyun sürüsünden alından 272 rektal sıvaptan izole edilen 215 E. coli izolatlarında STEC suşlarının virulens genleri ile shiga toksin alt tipleri tanımlanmıştır. 215 adet izolattan sadece 19 (%8,84) adet Stx1, 3 (%1,4) adet Stx2, 5 (%2,33) adet eae ve 9 (%4,19) adet ehxa geni, 13 (%6,05) adet Stx1-Stx2-ehxa, 5 (%2,33) adet Stx1-eae -ehxa, 3 (%1,4) adet Stx2-eae-ehxa birlikte ve 1(%0,47) adet Stx1- eae, 30 (%13,95) adet Stx1- ehxa, 1(%0,47) adet Stx2- eae, 15 (%6,98) adet Stx2-ehxa ve 10 (%4,65) adet eae – ehxa birlikte tespit edilmiştir. 101 (%46,98) adet izolattan ise Stx1, Stx2, eae ve ehxa genlerinden herhangi biri tespit edilmemiştir. Koyunlarda stx1 alt tiplendirmesinde ise yüksek oranda (%82,35) stx1c izole edilmiştir. Stx2 alt tiplendirmesi de ise sırasıyla stx2f (%62,86), stx2c (%54,29), stx2b (%45,71), stx2d (%22,86), stx2h (%22,86) alt tipleri farklı kombinasyonlarda daha yüksek oranda tespit edilmiştir. Bu veriler koyunlarda STEC varlığını ve insanlarda ciddi enfeksiyona neden olma potansiyellerinin olduğunu göstermektedir.

Anahtar Kelimeler:

Shiga Toksin Üreten E. coli (STEC), Stx1, Stx2, Alt Tip, Koyunlar

Investigation of virulence factors of shiga toxin-producing Escherichia coli strains isolated from sheep

All over the world, Escherichia coli (STEC), which produces Shiga toxin, is recognized as an important zoonotic pathogen. Ruminants, particularly cattle, are their main reservoirs, but the role of small ruminants in the epidemiology of human infections has not been extensively evaluated in many countries. In this study, it was aimed to determine the Shiga toxin subtypes of Stx1, Stx2, eae and ehxa genes of Shiga toxin producing isolates within Escherichia coli strains in sheep and to determine the comparative effect of variables such as race and sex on them. In this study, virulence genes of STEC strains and Shiga toxin subtypes were identified in 215 E. coli isolates isolated from 272 rectal swab from 12 different sheep herds in and around Ankara. Of the 215 isolates, only 19 (8.84%) Stx1, 3 (1.4%) Stx2, 5 (2.33%) eae and 9 (4.19%) ehxa genes, 13 (6%, 05) Stx1-Stx2-ehxa, 5 (2.33%) Stx1-eae -ehxa, 3 (1.4%) Stx2-eae-ehxa together and 1 (0.47%) Stx1- eae, 30 (13.95%) Stx1- ehxa, 1 (0.47%) Stx2- eae, 15 (6.98%) Stx2-ehxa and 10 (4.65%) eae – ehxa were detected together. Of the 101 (46.98%) isolates, none of the Stx1, Stx2, eae and ehxa genes were detected. In stx1 subtyping in sheep, a high rate (82.35%) of stx1c was isolated. In stx2 subtyping stx2f (%62,86), stx2c (%54,29), stx2b (%45,71), stx2d (%22,86), stx2h (%22,86) subtypes are more common in different combinations. Detected at a high rate. These data indicate the presence of STEC in sheep and their potential to cause serious infection in humans

Keywords:

Shiga Toxin Producing E. coli, Stx1, Stx2, Subtype, Sheep.,

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