Hamza KADI, Cüneyt TAMER, Emre OZAN, Mitat KURT, Selma KAYA, Abdullah CAVUNT, Harun ALBAYRAK

Sığırların Nodüler Ekzantemi hastalığında vektör kene ile konak arasındaki ilişkinin moleküler araştırılması

Poxviridae familyasının capripoxvirus cinsine ait olan Sığırların Nudüler Ekzantemi Hastalığı Virusu (SNEHV), özellikle Afrika ve Ortadoğu ülkelerinde sığırlarda önemli enfeksiyonlara neden olmaktadır. SNEH, birincil enfeksiyon kaynağı olan kan emici sineklerle yayılan arboviral bir hastalıktır. Ancak deneysel çalışmalarda kenelerde SNHEV'ye ait nükleik asit saptanmış ve bulaşmada kenelerin de rol oynayabileceği bildirilmiştir. Bu çalışma kapsamında, Türkiye’de Karadeniz Bölgesi’nde yeralan ve SNEH enfeksiyonlarının yoğun olarak tespit edildiği Samsun,Sivas ve Tokat illerinden sığır, koyun ve keçilerden kan ve kene örnekleri toplanarak hastalığın vektör-kene-konak ilişkisinin incelenmesi amaçlanmıştır. Bu amaçla Mart 2016-Ekim 2017 tarihleri arasında kene enfestasyonu olan 88 sığır, 511 koyun ve 108 keçiden kene ve kan örnekleri alındı. Kan örnekleri alınan bu hayvanlardan toplam 2508 kene toplandı ve keneler türlerine göre sınıflandırıldı. Real time PCR ile SNHEV nükleik asitlerinin varlığını ortaya çıkarmak için kan örnekleri ve keneler test edildi ve hem kan örneklerinde hem de kenelerde SNHEV nükleik asitleri tespit edilemedi.

Anahtar Kelimeler:

SNEHV, Kene, Vektör, real time PCR

Molecular investigation of the relationship between vector tick and host in Lumpy Skin Disease

Lumpy Skin Diseases Virus (LSDV), belonging to the capripoxvirus genus of the Poxviridae family, causes significant infections in cattle, especially in African and Middle East countries. LSD is an arboviral disease that spreads with bloodsucking flies as the primary source of infection. However, in experimental studies, nucleic acid belonging to the LSDV has been detected in ticks, and it has been reported that ticks may also play a role in transmission. Within the scope of this study, we have aimed to investigate the vector tick-host relationship of the disease by collecting blood and tick samples from cattle, sheep, and goats in Samsun, Sivas, and Tokat provinces, where LSD infections are intensely detected in the Black Sea Region in Turkey. For this purpose, ticks and blood samples were collected from 88 cattle, 511 sheep, and 108 goats with tick infestation between March 2016 and October 2017. A total of 2508 ticks were collected from these animals, whose blood samples with EDTA were taken, and the ticks were classified according to species. Blood samples and ticks were tested to reveal the presence of LSDV nucleic acids by real-time PCR, and LSDV nucleic acids could not be detected in both blood samples or ticks.

Keywords:

LSDV, Tick, Vector, real-time PCR SNEHV, Kene, Vektör, real time PCR,

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