Tavuk (Gallus domesticus) alt solunum yollarındaki mast hücreleri üzerinde histolojik araştırmalar

Bu araştırma tavukların (Gallus domesticus) alt solunum yollarındaki mast hücrelerinin ışık ve elektron mikroskobik düzeylerde morfolojisi ve heterojenitesini ortaya koymak amacıyla yapıldı. Çalışmada 20 adet sağlıklı, erişkin broyler tipi tavuğun alt solunum yollarından alınan doku örnekleri materyal olarak kullanıldı. Işık mikroskobik düzeyde yapılan incelemeler sonucunda izotonik formaldehit-asetik asit (IFAA) ile tespit edilen dokulardaki mast hücrelerinin uzun ve kısa süreli toluidin blue boyaması ile metakromatik, alcian blue/safranin O (AB/SO) kombine boya metodu ile AB (+), SO (+) ve AB/SO (+) (mikst) olarak boyandığı gözlendi. %10 formol ile tespit edilen mast hücreleri ise, uzun süreli toluidin blue boyamasında metakromatik özellik göstermeksizin mavi renkte buyandılar. Elektron mikroskobik incelemeler sonucunda mast hücrelerinin homojen elektron yoğun, elektron açık matriks içinde elektron yoğun kordon ağı şeklinde, ince tanecikli yapıda, bal peteği görünümünde ve konsantrik lameller şeklinde olmak üzere beş farklı tipte granül içerdiği gözlendi.

Histological investigation on the mast cells of lower respiratory tract in the chicken (Gallus domesticus

This study was carried out to investigate morphology and heterogeneity of mast cells in Broiler chicken's lower respiratory tract at the light and electron microscopic level. In this study, lower respiratory tract samples taken from 20 healthy, adult Broiler chickens. As a result of light microscopical examinations, observed the mast cells in isotonic formaldehyde-acetic acid (IFAA)-fixed were stained metachromatically with'short and long time toluidin blue methods and AB (+), SO (+), AB/SO (+) (mixed) with alcian blue/safranin O (AB/SO) combined staining method. However, the mast cells in %10 formol-fixed were stained blue but not metachromatically with long time toluidin blue method. As a result of electron microscopical examinations, saw the mast cells have five types granules: homogen electron dense, networks of cords formed by electron dense materials in electron clear matrix, fine granular, consentric lamellae and honeycomb appearence.

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