The effects of 1 mT and 5 mT static magnetic fields on platelet aggregation

Giriş: Statik manyetik alanların biyolojik etkileri, birçok çalışmada farklı hücreler ve fonksiyonlar üzerinden gösterilmiştir. Bizim bu çalışmadaki amacımız, 1 mT ve 5 mT statik manyetik alanların trombosit agregasyonu üzerine etkisini araştırmaktır. Gereç ve Yöntem: 18 sağlıklı gönüllüden alınan kan örnekleri sitratla veya heparinle antikoagüle edildi. Antikoagüle tam kandan elde edilen trombositten zengin plazma örnekleri 4'e bölündü. İkisine Helmholtz bobinde 1 saat, 1 veya 5 mT statik manyetik alan uygulanırken diğer ikisi kontrol grubu oldu. Manyetik alan ve kontrol grubu trombositten zengin plazma örnekleri aynı anda 4 kanallı optik agregometrede çalışıldı. Trombosit agregasyonunu uyarmak için ADP (2-4 μM), Kollajen (38-95 μg/ml) ve Epinefrin (10 μM) kullanıldı. Agregasyon eğrilerinin eğimleri, maksimum değerleri ve lag zamanları, kontrol ve manyetik alan grupları arasında karşılaştırıldı. Bulgular: Oluşturulan 4 grupta [1 mT sitrat (n=5), 5 mT sitrat (n=5), 1 mT heparin (n=5), 5 mT heparin (n=5)] manyetik alan ve kontrol grupları arasında istatistiksel olarak anlamlı bir farklılığa rastlanmadı. Sonuç: 1 saat uygulanan, 1 mT veya 5 mT statik manyetik alanların trombosit agregasyonu üzerine etkisi gözlenmedi.

1 mT ve 5 mT statik manyetik alanların trombosit agregasyonuna etkisi

Aim: Numerous studies have been conducted on the biological effects of static magnetic fields, reporting effects on various cell types and functions. The aim of this study is to investigate the biological effects of 1 mT and 5 mT static magnetic fields using platelet aggregation. Materials and Methods: Blood samples taken from 18 healthy volunteers were anti-coagulated with citrate or with heparin. The platelet rich plasma obtained from the anti-coagulated whole blood was divided into four equal parts. Two were exposed to 1 mT or 5 mT static magnetic fields produced by a Helmholtz coil for 1 hour; the remaining two were assigned as control groups. Both groups were simultaneously studied with a 4-channel optical aggregometer. ADP (2-4 μM), Collagen (38-95 μg/ml) and Epinephrine (10 μM) were used to induce platelet aggregation. The slopes, maximum values, and lag times for the aggregation curves for the control samples and the exposure groups were compared. Results: No statistically significant differences were observed for any of the four groups formed: 1 mT-citrate (n=5), 5 mT-citrate (n=5), 1 mT-heparin (n=5), 5 mT-heparin (n=5). Conclusion: 1-hour, 1 mT or 5 mT static magnetic field exposures have no effect on the platelet aggregation.

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